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Plasmid-Reference_book.png Plasmids for Stem Cell Research


Stem cells are proliferative, unspecialized cells with the capacity to differentiate into many different cell types in the body during development. Unlike other cell types, each stem cell has the potential to either remain a stem cell or become another type of cell with a more specialized function. They also have the unique potential to continuously divide while maintaining an undifferentiated state. Stem cells are classified as pluripotent or multipotent, based on their ability to generate different cell lineages. Pluripotent stem cells, such as embryonic cells, can potentially give rise to any other cell type in the body. Multipotent stem cells, such as adult (or somatic) stem cells, can differentiate into multiple cell types, but are typically limited to related cell lineages.

Schematic of reprogramming stem cells

Pluripotent embryonic and multipotent adult stem cells have been studied for over 30 years. All of this research led to Kazutoshi Takahashi and Shinya Yamanaka’s breakthrough development of induced pluripotent stem cells (iPSCs) in both mouse and human cells in 2006 and 2007, respectively. To create iPSCs, fully-differentiated adult somatic cells are returned to a pluripotent state using a cocktail of factors (Oct3/4, Sox2, c-Myc, and Klf4) that are known to maintain pluripotency during embryonic development. This “reprogramming” of the cell to an embryonic state holds immense potential for drug development, disease modeling, and regenerative medicine.

iPSCs, much like their ES cell cousins, have the hallmarks of pluripotent cells--they are capable of differentiating into every other cell type in the body and have the capacity to give rise to an entire organism. iPSC technology removes the possible ethical concerns related to embryonic stem cell use and has the major advantage that iPSCs can potentially be derived from patient-specific or disease-specific cells. For this reason, iPSCs have become an excellent unlimited stem cell source for therapeutic use.

Since 2006, scientists have developed many approaches to generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and wait for cells to de-differentiate. However it may be difficult to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs provides an overview of several different reprogramming methods with the goal of helping readers choose a strategy suited to their research. Once you know which method you would like to use, browse Addgene's collection of Reprogramming Plasmids in the table below.

Once iPSCs have been created they can be directly differentiated into specific somatic stem cells or fully differentiated cell types. Browse Addgene's collection of Differentiation Plasmids below.

Alternatively, it is also possible to directly differentiate one differentiated somatic cell into another via transdifferentiation. This method of cell lineage conversion can be faster, bypasses intermediate pluripotent stages, and can occur without cell division, all of which lower the risk of mutation and minimize tumorigenicity, an inherent disadvantage of iPS cell technology. Browse Addgene's collection of Transdifferentiation Plasmids below.

Many of the techniques for reprogramming, differentiation, and transdifferentiation utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available from Addgene that can be used to create iPSCs or another cell type. You can also browse Addgene’s entire collection of Stem Cell Research Plasmids. For more information on stem cells, visit the NIH Stem Cell information page.

Reprogramming Plasmids

Browse the table below for plasmids used to create induced pluripotent stem cells (iPSCs). Sort the table by delivery method, species, or PI and then select the article link to find more information.

Delivery Method Species Description Article PI
Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc, and Brd3R from five separate lentiviral plasmids The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016 Mar 7;7:10869. Hu
CRISPRa Human CRISPR activator system for reprogramming human cells to pluripotency by activating enogenous genes Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski
Lentivirus Human Expression of human Sox2, Nanog, Oct4, and Lin28 from four separate lentiviral plasmids Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Dec 21;318(5858):1917-20. Thomson
Lentivirus Human Doxycycline-inducible expression of human Oct4, Sox2, Klf4, and c-Myc from four separate lentiviral plasmids A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell. 2008 Sep 11. 3(3):346-53. Jaenisch
Lentivirus Human Single polycistronic lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem Cells. 2009 May . 27(5):1042-9. Townes
Lentivirus Human Single polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and c-Myc Human Induced Pluripotent Stem Cells Produced Under Xeno-Free Conditions. Stem Cells Dev. 2010 Aug;19(8):1221-9. Cibelli
Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency. Cell. 2015 Jul 16;162(2):412-24. Mikkelsen
Lentivirus Human Expression of human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions in single lentiviral vectors for human iPSC generation OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. Ptashne
Minicircle Human Non-integrating mini-intronic plasmid (MIP) polycistronic expression of human Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Sci Rep. 2015 Jan 28;5:8081. Kay and Wu
MMLV-derived Retrovirus Human Yamanaka factors for generating human iPS cells; retroviral expression of human Sox2, Oct3/4, Klf4, and c-Myc from four separate plasmids Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007 Nov 30. 131(5):861-72. Yamanaka
Plasmid Human Non-integrating transient expression of human L-Myc, Lin28, Sox2, Klf4, Oct3/4, Glis1, and EBNA1 from separate plasmids An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. Stem Cells. 2012 Nov 29. Yamanaka
Replicating EBNA1 episome Human Non-integrating EBNA1-mediated polycistronic expression of human Sox2, KLF4, L-Myc, Lin28, OCT3/4, and shRNA against p53 in different gene/insert combinations. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 May;8(5):409-12. Yamanaka
Replicating EBNA1 episome Human Non-integrating polycistronic expression of human Oct4, Klf4, Sox2, c-Myc, Nanog, Lin28, NR5A2, and microRNA 302/367 in three different combinations of reprogramming factors Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi
Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3/4, shp53, Klf4, Sox2, L-Myc, Lin28 from separate non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming. Stem Cell Res Ther. 2017 Jun 5;8(1):132. Zovein
Replicating EBNA1 episome Human Non-integrating EBNA1-mediated polycistronic expression of human Oct4, Sox2, Myc, Klf4, BCL2L1, and EBNA1 from three separate plasmids A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214. Cheng
RNA Human Reprogramming factors for delivering synthetic mRNAs encoding human Klf4, Oct4, Sox2, c-Myc, and Lin28A to somatic cells from separate plasmids Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Cell Stem Cell. 2010 Nov 5;7(5):618-30. Rossi
RNA Human Non-integrating, polycistronic, self-replicating VEE RNA species expressing human Oct4, Klf4, Sox2, with either c-Myc or Glis1 Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell. 2013 Aug 1;13(2):246-54. Dowdy
Adenovirus Mouse Non-integrating expression of mouse Sox2, Oct4, c-Myc, and Klf4 from four separate adenoviral plasmids Induced Pluripotent Stem Cells Generated Without Viral Integration. Science. 2008 Nov 7;322(5903):945-9. Hochedlinger
Lentivirus Mouse Doxycycline-inducible expression of mouse Oct4, Sox2, Klf4, and c-Myc from four separate lentiviral plasmids Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell. 2008 Feb 7. 2(2):151-9. Jaenisch
Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. Jaenisch
Lentivirus Mouse Doxycycline-inducible lentiviral expression of mouse Oct4, Sox2, Klf4, and c-Myc from four separate plasmids Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell. 2008 Mar 6. 2(3):230-40. Hochedlinger
MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells; retroviral expression of mouse Sox2, Oct3/4, Klf4, and c-Myc from separate plasmids Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25. 126(4):663-76. Yamanaka
PiggyBac Mouse Doxycycline-inducible piggyBac transposon reprogramming system; expression of mouse Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. Woltjen
Plasmid Mouse Non-integrating polycistronic expression of mouse Oct4, Klf4, Sox2, and c-Myc from two separate plasmids Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors. Science. 2008 Nov 7;322(5903):949-53. Yamanaka
Replicating EBNA1 episome Mouse Non-integrating EBNA1-mediated polycistronic expression of mouse Oct4, Sox2, Klf4, c-Myc, and Lin28 Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell Res. 2011 Mar;21(3):518-29. Cheng

You can also find gene-specific plasmids at the following links: NANOG, OCT4, SOX2, MYC, KLF4, LIN28.

Differentiation and Transdifferentiation Plasmids

Browse the table below for plasmids used for differentiation of iPSCs or transdifferentiation of a somatic cell to a new type of somatic cell. Sort the table by cell type, delivery system, species, or PI and then select the article link to find more information.

From → To Delivery Method Species Article PI
Fibroblasts → iPSCs, iTSCs, and iXENs Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun6. Buganim
iPSC → Cardiomyocyte Lentiviral Human Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek
iPSC → Bipolar Neurons Lentiviral Human Rapid neurogenesis through transcriptional activation in human stem cells. Mol Syst Biol. 2014 Nov 17;10(11):760. Weiss
iPSC → Cortical or Lower Motor Neurons CRISPR/TALEN Human Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons. Curr Protoc Cell Biol. 2018 Jun;79(1):e51.

Next generation vectors for transcription factor mediated differentiation of iPSCs into cortical and motor neurons. Unpublished.
Ward
Fibroblast → Motor Neurons Retroviral Mouse/Human Conversion of mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):205-18. Eggan
Fibroblast → Neural Stem Cell Retroviral Mouse Direct reprogramming of fibroblasts into neural stem cells by defined factors. Cell Stem Cell. 2012 Apr 6;10(4):465-72. Scholer
Fibroblasts → Hepatocytes Retroviral Mouse Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature. 2011 Jun 29;475(7356):390-3. Suzuki
Fibroblast → Neurons Lentiviral Mouse Direct conversion of fibroblasts to functional neurons by defined factors. Nature. 2010 Feb 25. 463(7284):1035-41 Wernig
Fibroblast → Neural Stem Cell Lentiviral Mouse Direct Lineage Conversion of Adult Mouse Liver Cells and B Lymphocytes to Neural Stem Cells. Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. Jaenisch
Fibroblast → Sensory Neurons Lentiviral Mouse Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci. 2015 Jan;18(1):25-35. Baldwin
Fibroblast → Astrocyte Lentiviral Mouse Direct conversion of fibroblasts into functional astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1):25-36. Broccoli
Fibroblast → Oligodendrocyte Progenitor Lentiviral Mouse Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol. 2013 May;31(5):426-33. Tesar
Fibroblast → Cardiomyocyte Lentiviral Mouse Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Mol Cell Cardiol. 2013 Apr 13;60C:97-106. Gearhart
Fibroblast → Sertoli Cell Lentiviral Mouse Direct Reprogramming of Fibroblasts into Embryonic Sertoli-like Cells by Defined Factors. Cell Stem Cell. 2012 Sep 7;11(3):373-86. Jaenisch
Fibroblast → Hematopoietic Progenitor Lentiviral Mouse Direct reprogramming of murine fibroblasts to hematopoietic progenitor cells. Cell Rep. 2014 Dec 11;9(5):1871-84. Lacaud
Pancreatic Exocrine → Beta-cell Adenoviral Mouse In vivo reprogramming of adult pancreatic exocrine cells to beta-cells. Nature. 2008 Aug 27. Melton
Skin Fibroblast → Motor Neuron Lentiviral Human Direct Lineage Reprogramming Reveals Disease-Specific Phenotypes of Motor Neurons from Human ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang
Adult Skin Fibroblast → Cholinergic Neuron Lentiviral Human Small molecules enable neurogenin 2 to efficiently convert human fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang
Fetal Lung Fibroblast → Cholinergic Neuron Lentiviral Human Small molecules enable neurogenin 2 to efficiently convert human fibroblasts into cholinergic neurons. Nat Commun. 2013;4:2183. Zhang
Fibroblast → Dopaminergic Neuron Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8. Parmar
Adult Dermal Fibroblast → Neuron Lentiviral Human REST suppression mediates neural conversion of adult human fibroblasts via microRNA-dependent and -independent pathways. EMBO Mol Med. 2017 Aug;9(8):1117-1131. Parmar
Astrocytes → Dopaminergic Neuron Lentiviral Human Efficient conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS One. 2011;6(12):e28719. Gearhart

Stem Cell Research Plasmids

This table contains a general list of plasmids that have been used in Stem Cell research. You can search or sort this table based on plasmid name, species, gene name, and more.

Plasmid Gene/Insert Vector Type PI Publication
pWZL Blast mycc-myc (Homo sapiens)Mammalian Expression, Retroviral Hahn Transformation of human and murine fibroblasts without viral oncoproteins. Mol Cell Biol. 2005 Aug . 25(15):6464-74.
RCAS-mycc-myc (Homo sapiens)Retroviral Orsulic Induction of ovarian cancer by defined multiple genetic changes in a mouse model system. Cancer Cell. 2002 Feb . 1(1):53-62.
pMXs-NanogNanog homeobox (Mus musculus)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25. 126(4):663-76.
pMXs-Oct3/4POU domain, class 5, transcription factor 1 (Mus musculus)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25. 126(4):663-76.
pMXs-Sox2SRY-box containing gene 2 (Mus musculus)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25. 126(4):663-76.
pMXs-Klf4Kruppel-like factor 4 (gut) (Mus musculus)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25. 126(4):663-76.
pMXs-c-Mycmyelocytomatosis oncogene (Mus musculus)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25. 126(4):663-76.
pCAG-HA-Sox2-IPSox2 (Mus musculus)Mammalian Expression Yamanaka Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells. J Biol Chem. 2005 Jul 1. 280(26):24371-9.
pCAG-Myc-Oct3/4-IPOct3/4 (Mus musculus)Mammalian Expression Yamanaka Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells. J Biol Chem. 2005 Jul 1. 280(26):24371-9.
pCAG-Oct3/4-IRES-NeoOct3/4 (Mus musculus)Mammalian Expression Yamanaka Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Mol Cell Biol. 2003 Apr . 23(8):2699-708.
pCAG-Sox2-IRES-NeoSox2 (Mus musculus)Mammalian Expression Yamanaka Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Mol Cell Biol. 2003 Apr . 23(8):2699-708.
pTV-Nanog HygIRES-Hyg-pA cassette flanked by Nanog homologous regions (Mus musculus)Mammalian Expression ; Beta-gal Yamanaka The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 2003 May 30. 113(5):631-42.
pTV-Nanog BgeoIRES-Bgeo-pA cassette flanked by Nanog homologous regions (Mus musculus)Mammalian Expression ; Beta-gal Yamanaka The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 2003 May 30. 113(5):631-42.
pPyCAG-Nanog-IPNanog (Mus musculus)Mammalian Expression Yamanaka The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 2003 May 30. 113(5):631-42.
pDEST17-NanogNanog (Mus musculus)Bacterial Expression Yamanaka The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 2003 May 30. 113(5):631-42.
pBS human c-Mycc-Myc (Homo sapiens)Bacterial Expression Massague Mad upregulation and Id2 repression accompany transforming growth factor (TGF)-beta-mediated epithelial cell growth suppression. J Biol Chem. 2003 Sep 12. 278(37):35444-50.
pBS mouse c-Mycc-Myc (Mus musculus)Bacterial Expression Massague Mad upregulation and Id2 repression accompany transforming growth factor (TGF)-beta-mediated epithelial cell growth suppression. J Biol Chem. 2003 Sep 12. 278(37):35444-50.
pMXs-Oct3/4-IPPOU domain, class 5, transcription factor 1 (Mus musculus)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25. 126(4):663-76.
pMXs-Sox2-IPSRY-box containing gene 2 (Mus musculus)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25. 126(4):663-76.
pMXs-Klf4-IPKruppel-like factor 4 (gut) (Mus musculus)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25. 126(4):663-76.
pMXs-c-Myc-IPmyelocytomatosis oncogene (Mus musculus)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006 Aug 25. 126(4):663-76.
pLOVE-Klf4Kruppel-like factor 4 (Mus musculus)Mammalian Expression, Lentiviral Ramalho-Santos Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Cell Stem Cell. 2007 Sep 13. ():.
pLOVE-Nmycv-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mus musculus)Mammalian Expression, Lentiviral Ramalho-Santos Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Cell Stem Cell. 2007 Sep 13. ():.
pLOVE-Sox2SRY (sex determining region Y)-box 2 (Mus musculus)Mammalian Expression, Lentiviral Ramalho-Santos Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Cell Stem Cell. 2007 Sep 13. ():.
pcDNA3-cmyccmyc (Homo sapiens)Mammalian Expression El-Deiry Direct repression of FLIP expression by c-myc is a major determinant of TRAIL sensitivity. Mol Cell Biol. 2004 Oct . 24(19):8541-55.
pGEM-LIN28Homo sapiens lin-28 homolog (C. elegans) (Homo sapiens)Bacterial Expression Thomson Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():.
pGEM-NanogHomo sapiens Nanog homeobox (Homo sapiens)Bacterial Expression Thomson Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():.
pGEM-Oct4Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (Homo sapiens)Bacterial Expression Thomson Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():.
pGEM-Sox2Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (Homo sapiens)Bacterial Expression Thomson Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():.
deltaMyc-HAc-MYC (Homo sapiens)Mammalian Expression Vogelstein Identification of CDK4 as a target of c-MYC. Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34.
pSin-EF2-Sox2-PurHomo sapiens SRY (sex determining region Y)-box 2 (SOX2) (Homo sapiens)Mammalian Expression, Lentiviral Thomson Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():.
pSin-EF2-Nanog-PurHomo sapiens Nanog homeobox (Homo sapiens)Mammalian Expression, Lentiviral Thomson Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():.
pSin-EF2-Oct4-PurHomo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (Homo sapiens)Mammalian Expression, Lentiviral Thomson Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():.
pSin-EF2-LIN28-PurHomo sapiens lin-28 homolog (C. elegans) (Homo sapiens)Mammalian Expression, Lentiviral Thomson Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():.
pMXs-hOCT3/4OCT3/4 (Homo sapiens)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007 Nov 30. 131(5):861-72.
pMXs-hSOX2SOX2 (Homo sapiens)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007 Nov 30. 131(5):861-72.
pMXs-hKLF4KLF4 (Homo sapiens)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007 Nov 30. 131(5):861-72.
pMXs-hc-MYCc-MYC (Homo sapiens)Mammalian Expression, Retroviral Yamanaka Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007 Nov 30. 131(5):861-72.
pMIG-hOCT4POU class 5 homeobox 1 (Homo sapiens)Mammalian Expression, Retroviral Daley Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 2008 Jan 10. 451(7175):141-6.
pMIG-hSOX2SRY (sex determining region Y)-box 2 (Homo sapiens)Mammalian Expression, Retroviral Daley Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 2008 Jan 10. 451(7175):141-6.
pMIG-hKLF4Kruppel-like factor 4 (gut) (Homo sapiens)Mammalian Expression, Retroviral Daley Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 2008 Jan 10. 451(7175):141-6.
pBabe-c-myc-zeoc-myc (Homo sapiens)Mammalian Expression, Retroviral Lindquist Heat shock factor 1 is a powerful multifaceted modifier of carcinogenesis. Cell. 2007 Sep 21. 130(6):1005-18.
pMXs-hOCT4 (Plath)POU domain, class 5, transcription factor 1 (Homo sapiens)Mammalian Expression, Retroviral Plath Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8.
pMXs-hSOX2 (Plath)SRY (sex determining region Y)-box 2 (Homo sapiens)Mammalian Expression, Retroviral Plath Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8.
pMXs-hcMYC (Plath)v-myc myelocytomatosis viral oncogene homolog (avian) (Homo sapiens)Mammalian Expression, Retroviral Plath Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8.
pMXs-hKLF4 (Plath)Kruppel-like factor 4 (gut) (Homo sapiens)Mammalian Expression, Retroviral Plath Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8.
pMXs-hNANOG (Plath)Nanog homeobox (Homo sapiens)Mammalian Expression, Retroviral Plath Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8.
MSCV h c-MYC IRES GFPc-MYC (Homo sapiens)Retroviral Cleveland Cleveland lab plasmids (unpublished)
tetO7_Ef1a_OCT4OCT4 (Homo sapiens)Mammalian Expression, Lentiviral, Cre/Lox Cheng Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts. Stem Cells. 2008 Aug . 26(8):1998-2005.
tetO7_Ef1a_SOX2SOX2 (Homo sapiens)Mammalian Expression, Lentiviral, Cre/Lox Cheng Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts. Stem Cells. 2008 Aug . 26(8):1998-2005.
Ef1a_NANOG_Ires_PuroNANOG (Homo sapiens)Mammalian Expression, Lentiviral, Cre/Lox Cheng Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts. Stem Cells. 2008 Aug . 26(8):1998-2005.
Ef1a_LIN28_Ires_PuroLIN28 (Homo sapiens)Mammalian Expression, Lentiviral, Cre/Lox Cheng Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts. Stem Cells. 2008 Aug . 26(8):1998-2005.
pBabepuro-myc-ERc-myc (Homo sapiens)Mammalian Expression, Retroviral El-Deiry Direct repression of FLIP expression by c-myc is a major determinant of TRAIL sensitivity. Mol Cell Biol. 2004 Oct . 24(19):8541-55.
pLV-tetO-myc T58Ac-myc (Homo sapiens)Mammalian Expression, Lentiviral Hochedlinger Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell. 2008 Mar 6. 2(3):230-40.
pLV-tetO-Klf4Klf4 (Mus musculus)Mammalian Expression, Lentiviral Hochedlinger Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell. 2008 Mar 6. 2(3):230-40.
pLV-tetO-Sox2Sox2 (Mus musculus)Mammalian Expression, Lentiviral Hochedlinger Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell. 2008 Mar 6. 2(3):230-40.
pLV-tetO-Oct4Oct4 (Mus musculus)Mammalian Expression, Lentiviral Hochedlinger Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell. 2008 Mar 6. 2(3):230-40.
pAd-Sox2Sox2 (Mus musculus)Mammalian Expression, Adenoviral Hochedlinger Induced Pluripotent Stem Cells Generated Without Viral Integration. Science. 2008 Sep 25. ():.
pAd-Oct4Oct4 (Mus musculus)Mammalian Expression, Adenoviral Hochedlinger Induced Pluripotent Stem Cells Generated Without Viral Integration. Science. 2008 Sep 25. ():.
pAd-cMyc T58Ac-myc (Homo sapiens)Mammalian Expression, Adenoviral Hochedlinger Induced Pluripotent Stem Cells Generated Without Viral Integration. Science. 2008 Sep 25. ():.
pAd-Klf4Klf4 (Mus musculus)Mammalian Expression, Adenoviral Hochedlinger Induced Pluripotent Stem Cells Generated Without Viral Integration. Science. 2008 Sep 25. ():.
pCX-OKS-2AOct3/4-2A-Klf4-2A-Sox2 (Mus musculus)Mammalian Expression Yamanaka Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors. Science. 2008 Oct 9. ():.
pCX-cMycc-Myc (Mus musculus)Mammalian Expression Yamanaka Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors. Science. 2008 Oct 9. ():.
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FU-tet-o-hKLF4Klf4 (Homo sapiens)Mammalian Expression, Lentiviral Hochedlinger A high-efficiency system for the generation and study of human induced pluripotent stem cells. Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
FU-tet-o-hOct4Oct4 (Homo sapiens)Mammalian Expression, Lentiviral Hochedlinger A high-efficiency system for the generation and study of human induced pluripotent stem cells. Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
FU-tet-o-hSox2Sox2 (Homo sapiens)Mammalian Expression, Lentiviral Hochedlinger A high-efficiency system for the generation and study of human induced pluripotent stem cells. Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
pMSCV-Flag-cMyc T58AcMyc (Homo sapiens)Mammalian Expression, Retroviral Belmonte Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol. 2008 Nov . 26(11):1276-84.
pMSCVpuro-Flag-cMyc T58AcMyc (Homo sapiens)Mammalian Expression, Retroviral Belmonte Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol. 2008 Nov . 26(11):1276-84.
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TetO-FUW-oct4oct4 (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell. 2008 Feb 7. 2(2):151-9.
TetO-FUW-cMYCcMYC (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell. 2008 Feb 7. 2(2):151-9.
FUW-SOKMSox2-P2A-Oct4-T2A-Klf4-E2A-cMyc (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
TetO-FUW-sox2sox2 (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell. 2008 Feb 7. 2(2):151-9.
FUW-SOKSox2-F2A-Oct4-T2A-Klf4 (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
FUW-OSKMOct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
FUW-SOSox2-F2A-Oct4 (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
FUW-SMSox2-F2A-cMyc (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
FUW-KOKlf4-F2A-Oct4 (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
FUW-KMKlf4-F2A-cMyc (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
FUW-tetO-hMYCc-MYC (Homo sapiens)Mammalian Expression, Lentiviral Jaenisch A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell. 2008 Sep 11. 3(3):346-53.
FUW-tetO-hSOX2SOX2 (Homo sapiens)Mammalian Expression, Lentiviral Jaenisch A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell. 2008 Sep 11. 3(3):346-53.
FUW-tetO-hKLF4KLF4 (Homo sapiens)Mammalian Expression, Lentiviral Jaenisch A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell. 2008 Sep 11. 3(3):346-53.
FUW-tetO-hOCT4OCT4 (Homo sapiens)Mammalian Expression, Lentiviral Jaenisch A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell. 2008 Sep 11. 3(3):346-53.
FUW-tetO-lox-hKLF4KLF4 (Homo sapiens)Mammalian Expression, Lentiviral Jaenisch Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors Cell, Volume 136, Issue 5, 964-977, 6 March 2009
FUW-tetO-lox-hOCT4OCT4 (Homo sapiens)Mammalian Expression, Lentiviral Jaenisch Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors Cell, Volume 136, Issue 5, 964-977, 6 March 2009
FUW-tetO-lox-hSOX2SOX2 (Homo sapiens)Mammalian Expression, Lentiviral Jaenisch Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors Cell, Volume 136, Issue 5, 964-977, 6 March 2009
pCAGMKOSiEmyelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Mus musculus)Mammalian Expression Kaji Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature. 2009 Mar 1. ():.
pCAG2LMKOSimOc-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2-ires-mOrange (Mus musculus)Mammalian Expression Kaji Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature. 2009 Mar 1. ():.
PB-TET-mcMyccMyc (Mus musculus)Mammalian Expression ; piggyBac Nagy piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature. 2009 Mar 1. ():.
PB-TET-mKLF4KLF4 (Mus musculus)Mammalian Expression ; piggyBac Nagy piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature. 2009 Mar 1. ():.
PB-TET-mOct4Oct4 (Mus musculus)Mammalian Expression ; piggyBac Nagy piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature. 2009 Mar 1. ():.
PB-TET-mSox2Sox2 (Mus musculus)Mammalian Expression ; piggyBac Nagy piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature. 2009 Mar 1. ():.
pEP4 E02S EN2LOct4 and Sox2; Nanog and Lin28 (Homo sapiens)Episomal/EBNA Thomson Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26.
pEP4 E02S CK2M EN2LOct4 and Sox2; Nanog and Lin28; Klf4 and Myc (Homo sapiens)Episomal/EBNA Thomson Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26.
pEP4 E02S EN2KOct4 and Sox2; Nanog and Klf4 (Homo sapiens)Episomal/EBNA Thomson Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26.
pCEP4-M2LMYC (Homo sapiens), LIN28 (Homo sapiens)Episomal/EBNA Thomson Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26.
pEP4 E02S ET2KOct4 and Sox2; SV40LT and Klf4 (Homo sapiens)Episomal/EBNA Thomson Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26.
PB-TET-MKOScMyc, KLF4, Oct4, Sox2 (Mus musculus)Mammalian Expression ; piggyBac Nagy piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature. 2009 Mar 1. ():.
pBABE-puro-mouse Oct4Oct4 (Mus musculus)Mammalian Expression, Retroviral Tantin A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress. Genes Dev. 2009 Jan 15. 23(2):208-22.
pSIN4-EF2-O2SOct4, Sox2 (Homo sapiens)Lentiviral ; reprogramming Thomson Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26.
pSIN4-EF2-N2LNanog, Lin28 (Homo sapiens)Mammalian Expression, Lentiviral ; reprogramming Thomson Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26.
pSIN4-CMV-K2MKlf4, cMyc (Homo sapiens)Mammalian Expression, Lentiviral ; reprogramming Thomson Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26.
Oct4-ires-EGFP(lox neo)EGFP, Oct4 (Mus musculus)Mouse Targeting Jaenisch Oct4 expression is not required for mouse somatic stem cell self-renewal Cell Stem Cell. 2007 Oct 11;1(4):403-15.
pLM-vexGFP-Oct4vexGFP_2A_OCT4 (Homo sapiens)Lentiviral Sadelain Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
pLM-mCitrine-Sox2mCitrine_2A_SOX2 (Homo sapiens)Lentiviral Sadelain Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
pLM-mCherry-Klf4mCherry_2A_KLF4 (Homo sapiens)Lentiviral Sadelain Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
pLM-mCerulean-cMycmCerulean-2A-MYC (Homo sapiens)Lentiviral Sadelain Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
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HUW-TetO-hNanogNanog (Homo sapiens)Mammalian Expression, Lentiviral Jaenisch Direct cell reprogramming is a stochastic process amenable to acceleration. Nature. 2009 Dec 3. 462(7273):595-601.
pMXs-Hu-L-MycL-Myc (Homo sapiens)Mammalian Expression, Retroviral Yamanaka Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
pMXs-Ms-L-MycL-Myc (Mus musculus)Mammalian Expression, Retroviral Yamanaka Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
pMXs-Ms-c-Myc-W136Ec-Myc (Mus musculus)Mammalian Expression, Retroviral Yamanaka Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
pBRPyCAG-fmKlf4-DsRed-IpKlf4 (Mus musculus)Mammalian Expression, Cre/Lox Jaenisch Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs. Proc Natl Acad Sci U S A. 2010 May 18. 107(20):9222-7.
pWZL Sox2Sox2 (Homo sapiens)Mammalian Expression, Retroviral Meyerson SOX2 is an amplified lineage-survival oncogene in lung and esophageal squamous cell carcinomas. Nat Genet. 2009 Nov . 41(11):1238-42.
pWZL Sox2 R74PSox2 (Homo sapiens)Mammalian Expression, Retroviral Meyerson SOX2 is an amplified lineage-survival oncogene in lung and esophageal squamous cell carcinomas. Nat Genet. 2009 Nov . 41(11):1238-42.
pMSCV-mLin28Alin-28 homolog A (Mus musculus)Mammalian Expression, Retroviral Daley Lin28 promotes transformation and is associated with advanced human malignancies. Nat Genet. 2009 Jul . 41(7):843-8.
pBABE-hLin28Blin-28 homolog B (Homo sapiens)Mammalian Expression, Retroviral Daley Lin28 promotes transformation and is associated with advanced human malignancies. Nat Genet. 2009 Jul . 41(7):843-8.
pcDNA3.3_KLF45'UTR-KLF4-3'UTR (Homo sapiens)Mammalian Expression Rossi Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Cell Stem Cell. 2010 Sep 29. ():.
pcDNA3.3_OCT45'UTR-OCT4-3'UTR (Homo sapiens)Mammalian Expression Rossi Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Cell Stem Cell. 2010 Sep 29. ():.
pcDNA3.3_SOX25'UTR-SOX2-3'UTR (Homo sapiens)Mammalian Expression Rossi Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Cell Stem Cell. 2010 Sep 29. ():.
pcDNA3.3_c-MYC5'UTR-c-MYC-3'UTR (Homo sapiens)Mammalian Expression Rossi Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Cell Stem Cell. 2010 Sep 29. ():.
pCXLE-hOCT3/4OCT3/4 (Homo sapiens)Mammalian Expression Yamanaka A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 Apr 3. ():.
pCXLE-hOCT3/4-shp53-FOCT3/4 (Homo sapiens)Mammalian Expression Yamanaka A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 Apr 3. ():.
pCXLE-hSKSOX2, KLF4 (Homo sapiens)Mammalian Expression Yamanaka A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 Apr 3. ():.
pCXLE-hMLNC-MYC, LIN28, NANOG (Homo sapiens)Mammalian Expression Yamanaka A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 Apr 3. ():.
pCXLE-hULL-MYC (Homo sapiens)Mammalian Expression Yamanaka A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 Apr 3. ():.
pEB-C5Oct4, Sox2, Klf4, c-Myc, Lin28 (Mus musculus)Mammalian Expression Cheng Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell Res. 2011 Jan 18. ():.
pCDNA3.1-NanogNanog (Homo sapiens)Mammalian Expression Cheng Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell Res. 2011 Jan 18. ():.
pMXs-GLIS1GLIS1 (Homo sapiens)Mammalian Expression, Retroviral Yamanaka Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Nature. 2011 . 474(7350):225-9.
OCT4-eGFP-PGK-PuroOCT4 (Homo sapiens)targeting donor Jaenisch Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
OCT4-2A-eGFP-PGK-PuroOCT4 (Homo sapiens)targeting donor Jaenisch Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
OCT4-eGFP-2A-PuroOCT4 (Homo sapiens), eGFPtargeting donor Jaenisch Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
pMXs-mGLIS1GLIS1, Gateway casette A (Mus musculus)Mammalian Expression, Retroviral Yamanaka Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Nature. 2011 . 474(7350):225-9.
pMXs-GLIS1 (FLJ33315SAAN)GLIS1, Gateway casette A (Homo sapiens)Mammalian Expression, Retroviral Yamanaka Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Nature. 2011 . 474(7350):225-9.
pBpuro - HA KLF4 FLKLF4 (Homo sapiens)Mammalian Expression, Retroviral Ruppert A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
pBpuro - HA KLF4 FL delta K5SKLF4 (Homo sapiens)Mammalian Expression, Retroviral Ruppert A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
pcDNA3.1 - HA-KLF4 FLKLF4 (Homo sapiens)Mammalian Expression Ruppert A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
pcDNA3.1 - HA-KLF4 FL delta K5SKLF4 (Homo sapiens)Mammalian Expression Ruppert A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
pLJD-KLF4-ERKLF4 (Homo sapiens)Mammalian Expression, Retroviral Ruppert A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
pMIR-Report-Luc-KLF4-FLKLF4 (Homo sapiens)Mammalian Expression, Luciferase Ruppert A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
pBpuro_KLF4_ERKLF4 (Homo sapiens)Mammalian Expression, Retroviral Ruppert Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia. Oncogene. 2005 Feb 24;24(9):1491-500.
pLenti-EF1a-SOX2TALE-Sox2 (Synthetic)Mammalian Expression, Lentiviral Zhang Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():.
pLenti-EF1a-KLF4TALE-Klf4 (Synthetic)Mammalian Expression, Lentiviral Zhang Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():.
pLenti-EF1a-OCT4TALE-Oct4 (Synthetic)Mammalian Expression, Lentiviral Zhang Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():.
pCX4-Oct4Oct4 (Homo sapiens)Retroviral Dowdy Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells. Nature. 2012 Jan 25;482(7384):216-20. doi: 10.1038/nature10821.
pCX4-Sox2Sox2 (Homo sapiens)Retroviral Dowdy Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells. Nature. 2012 Jan 25;482(7384):216-20. doi: 10.1038/nature10821.
pCX4-KLF4kruppel-like factor 4 (Homo sapiens)Retroviral Dowdy Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells. Nature. 2012 Jan 25;482(7384):216-20. doi: 10.1038/nature10821.
pCX4-cMyccMyc (Homo sapiens)Retroviral Dowdy Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells. Nature. 2012 Jan 25;482(7384):216-20. doi: 10.1038/nature10821.
pWPXL-c-Mycc-Myc (Mus musculus)Mammalian Expression, Lentiviral Weinberg Slug and Sox9 cooperatively determine the mammary stem cell state. Cell. 2012 Mar 2;148(5):1015-28.
pWPXL-Klf4Klf4 (Mus musculus)Mammalian Expression, Lentiviral Weinberg Slug and Sox9 cooperatively determine the mammary stem cell state. Cell. 2012 Mar 2;148(5):1015-28.
pCXLE-hGLIS1human GLIS1 (Homo sapiens)Mammalian Expression Yamanaka An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
pCEP4_WT_OCT4POU5F1 (Homo sapiens)Mammalian Expression Thomson Phosphorylation regulates human OCT4. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2.
FUW-TetO-NanogNanog (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Single-Cell Expression Analyses during Cellular Reprogramming Reveal an Early Stochastic and a Late Hierarchic Phase. Cell. 2012 Sep 14;150(6):1209-22. doi: 10.1016/j.cell.2012.08.023.
pCE-hSKSOX2, KLF4 (Homo sapiens)Mammalian Expression Yamanaka An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
pCE-hULL-MYC, LIN28 (Homo sapiens)Mammalian Expression Yamanaka An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
pMYC-GFPc-Myc (Homo sapiens)Mammalian Expression, Bacterial Expression Olopade c-Myc activates BRCA1 gene expression through distal promoter elements in breast cancer cells. BMC Cancer. 2011 Jun 13;11:246. doi: 10.1186/1471-2407-11-246.
pD40-His/V5-c-Mycc-Myc (Mus musculus)Mammalian Expression Sears A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells. Nat Cell Biol. 2004 Apr;6(4):308-18. Epub 2004 Mar 14.
pMXs-mM₃O-IPmM₃O (Mus musculus)Mammalian Expression, Retroviral Kikyo Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD. Stem Cells. 2011 Sep;29(9):1349-61. doi: 10.1002/stem.684.
pMXs-hM₃O-IPhM₃O (Homo sapiens)Mammalian Expression, Retroviral Kikyo Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD. Stem Cells. 2011 Sep;29(9):1349-61. doi: 10.1002/stem.684.
pCDH-puro-cMycMYC (Homo sapiens)Mammalian Expression, Lentiviral Wang Inhibition of BET bromodomain targets genetically diverse glioblastoma. Clin Cancer Res. 2013 Apr 1;19(7):1748-59. doi: 10.1158/1078-0432.CCR-12-3066. Epub 2013 Feb 12.
Tet-pLKO-puro-SOX2SOX2 shRNA (Synthetic)Mammalian Expression, Lentiviral, RNAi Rudin Comprehensive genomic analysis identifies SOX2 as a frequently amplified gene in small-cell lung cancer. Nat Genet. 2012 Oct;44(10):1111-6. doi: 10.1038/ng.2405. Epub 2012 Sep 2.
Nanog-2A-mCherryNanog (Mus musculus)CRISPR Jaenisch One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering. Cell. 2013 Aug 27. pii: S0092-8674(13)01016-7. doi: 10.1016/j.cell.2013.08.022.
Oct4-IRES-eGFP-PGK-NeoOct4 (Mus musculus)CRISPR Jaenisch One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering. Cell. 2013 Aug 27. pii: S0092-8674(13)01016-7. doi: 10.1016/j.cell.2013.08.022.
pDNR-hSox2Sox2 (Homo sapiens)Cre/Lox ; donor vector McKinnon McKinnon Lab plasmids (unpublished)
pLPS-hSox2-gfpSox2 (Homo sapiens)Mammalian Expression, Cre/Lox ; acceptor vector McKinnon McKinnon Lab plasmids (unpublished)
pLP-hSox2-ires-gfpSox2 (Homo sapiens)Mammalian Expression, Cre/Lox ; acceptor vector McKinnon McKinnon Lab plasmids (unpublished)
pLP-TRE2-hSox2Sox2 (Homo sapiens)Mammalian Expression, Cre/Lox ; donor vector McKinnon McKinnon Lab plasmids (unpublished)
pMXs-hu-N-MycN-myc (Homo sapiens)Mammalian Expression, Retroviral Yamanaka Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
pMXs-hu-c-Myc-dN1c-Myc -dN1 (Homo sapiens)Mammalian Expression, Retroviral Yamanaka Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
pMXs-hu-c-Myc-dN2c-Myc -dN2 (Homo sapiens)Mammalian Expression, Retroviral Yamanaka Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
pMXs-ms-c-Myc-V394Dc-Myc V394D (Mus musculus)Mammalian Expression, Retroviral Yamanaka Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
pMXs-ms-c-Myc-L420Pc-Myc L420P (Mus musculus)Mammalian Expression, Retroviral Yamanaka Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
pMXs-ms-L-Myc-L351PL-Myc-L351P (Mus musculus)Mammalian Expression, Retroviral Yamanaka Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
pcDNA3-FLAG-Lin28ALin28A (Homo sapiens)Mammalian Expression Kim Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. Mol Cell. 2008 Oct 24;32(2):276-84. doi: 10.1016/j.molcel.2008.09.014.
pcDNA3-FLAG-Lin28A-mCCHCLin28A (Homo sapiens)Mammalian Expression Kim Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. Mol Cell. 2008 Oct 24;32(2):276-84. doi: 10.1016/j.molcel.2008.09.014.
pcDNA3-V5-Lin28ALin28A (Homo sapiens)Mammalian Expression Kim TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation. Cell. 2009 Aug 21;138(4):696-708. doi: 10.1016/j.cell.2009.08.002.
FUW-tetO-hOKMShOKMS (Homo sapiens)Mammalian Expression, Lentiviral Mikkelsen Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency. Cell. 2015 Jul 16;162(2):412-24. doi: 10.1016/j.cell.2015.06.016.
OCT4-GFP-2A-PURO transgenic reporterGFP-2A-puro (Synthetic), OCT4 (Homo sapiens)Mammalian Expression Hanna Derivation of novel human ground state naive pluripotent stem cells. Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30.
delta PE OCT4-GFP-2A-PURO transgenic reporterGFP-2A-puro (Synthetic), OCT4 (Homo sapiens)Mammalian Expression Hanna Derivation of novel human ground state naive pluripotent stem cells. Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30.
delta DE OCT4-GFP-2A-PURO transgenic reporterGFP-2A-puro (Synthetic), OCT4 (Homo sapiens)Mammalian Expression Hanna Derivation of novel human ground state naive pluripotent stem cells. Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30.
mouse deltaPE Oct4-GFP transgenic reporterGFP pA (Synthetic), Oct4 (Mus musculus), PGK-gb2-Neo (Synthetic)Mammalian Expression Hanna Derivation of novel human ground state naive pluripotent stem cells. Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30.
pGL3-human OCT4 DE-SV40-LucDistal element of human OCT4 promoter (Homo sapiens)Mammalian Expression, Luciferase Hanna Derivation of novel human ground state naive pluripotent stem cells. Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30.
pBABEpuro-CMYC T58ACMYC (Mus musculus)Mammalian Expression, Retroviral Counter Copper is required for oncogenic BRAF signalling and tumorigenesis. Nature. 2014 Apr 9. doi: 10.1038/nature13180.
pMaster1neo (Other), tk (Other), oct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2, myc (Homo sapiens), EBNA-1, Amp (Other), Lin28 (Homo sapiens), Nanog (Homo sapiens), IRES Capecchi Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7.
pMaster3neo (Other), tk (Other), oct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2, myc (Homo sapiens), EBNA-1, Amp (Other), Lin28 (Homo sapiens), Nanog (Homo sapiens), IRES, NR5A2 (Homo sapiens) Capecchi Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7.
pMaster12neo (Other), tk (Other), oct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2 (Homo sapiens), Myc (Homo sapiens), EBNA-1, Amp (Other), Lin28 (Homo sapiens), NANOG (Homo sapiens), IRES, NR5A2 (Homo sapiens), miR302/367 (Homo sapiens)Mammalian Expression Capecchi Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7.
T7-VEE-OKS-iMOct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2 (Homo sapiens), cMyc (Homo sapiens)Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon Dowdy Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001.
SP6-VEE-OKS-iMOct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2 (Homo sapiens), cMyc (Homo sapiens)Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon Dowdy Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001.
T7-VEE-OKS-iGOct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2 (Homo sapiens), GLIS (Homo sapiens)Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon Dowdy Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001.
SP6-VEE-OKS-iGOct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2 (Homo sapiens), GLIS (Homo sapiens)Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon Dowdy Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001.
pMXs-hGLIS1GRGLIS1 (Homo sapiens)Mammalian Expression, Retroviral Yamanaka Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm. Nat Commun. 2014 Apr 24;5:3678. doi: 10.1038/ncomms4678.
Nanog-2A-GFPNanog (Mus musculus)Mouse Targeting Jaenisch Single-cell analysis reveals that expression of nanog is biallelic and equally variable as that of other pluripotency factors in mouse ESCs. Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 10.1016/j.stem.2013.04.019.
Nanog-2A-mCherryNanog (Mus musculus)Mouse Targeting Jaenisch Single-cell analysis reveals that expression of nanog is biallelic and equally variable as that of other pluripotency factors in mouse ESCs. Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 10.1016/j.stem.2013.04.019.
Mouse Nanog locus 3' probeNanog (Mus musculus)Unspecified ; cloning Hanna Transient acquisition of pluripotency during somatic cell transdifferentiation with iPSC reprogramming factors. Nat Biotechnol. 2015 Jun 22. doi: 10.1038/nbt.3270.
FUW-TetO-Lin28Lin28 (Mus musculus)Mammalian Expression, Lentiviral Jaenisch Single-Cell Expression Analyses during Cellular Reprogramming Reveal an Early Stochastic and a Late Hierarchic Phase. Cell. 2012 Sep 14;150(6):1209-22. doi: 10.1016/j.cell.2012.08.023.
pPB-hCMV1-hNANOG-IVNANOG (Homo sapiens)Mammalian Expression Smith Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human. Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
pPB-PGK-hKLF4KLF4 (Homo sapiens)Mammalian Expression Smith Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human. Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
PpyCAG-NANOG-IBNANOG (Homo sapiens)Mammalian Expression Smith Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human. Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
pPyCAG-Nanog(T100A)-IPMouse Nanog T100A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(F102A)-IPMouse Nanog F102A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(Y119A)-IPMouse Nanog Y119A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(L122A)-IPMouse Nanog L122A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(Q124A)-IPMouse Nanog Q124A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(M125A)-IPMouse Nanog M125A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(Y136A)-IPMouse Nanog Y136A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(K137A)-IPMouse Nanog K137A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(Q138A)-IPMouse Nanog Q138A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(K140A)-IPMouse Nanog K140A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(T141A)-IPMouse Nanog T141A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(Q144A)-IPMouse Nanog Q144A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(N145A)-IPMouse Nanog N145A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(R147A)-IPMouse Nanog R147A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pPyCAG-Nanog(K151A)-IPMouse Nanog K151A Mutant (Mus musculus)Mammalian Expression Yamanaka Plasmids of NANOG HD Mutants (unpublished)
pP2A_NLS_sfGFPNanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm (Mus musculus)Just a donor DNA with Nanog homologous arm for knock-in sfGFP Qi Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells. Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 10.1016/j.stem.2015.01.003.
MIP 247 CoMiP 4in1 with shRNA p53Mini-intronic-plasmid intron (Homo sapiens)Mammalian Expression Kay Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
MIP 227 CoMiP 4in1 wo shRNA p53Mini-intronic-plasmid intron (Homo sapiens)Mammalian Expression Kay Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
MIP 339 CoMiP coLin28 2A Nanog IRES eGFPLin28 (Homo sapiens), Nanog (Homo sapiens)Mammalian Expression Kay Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
Luc-Nanog-3'UTRNanog homeobox (Mus musculus)Mammalian Expression He miR-34 miRNAs provide a barrier for somatic cell reprogramming. Nat Cell Biol. 2011 Oct 23;13(11):1353-60. doi: 10.1038/ncb2366.
Luc-Nanog-3'UTRmutNanog homeobox (Mus musculus)Mammalian Expression He miR-34 miRNAs provide a barrier for somatic cell reprogramming. Nat Cell Biol. 2011 Oct 23;13(11):1353-60. doi: 10.1038/ncb2366.
MOSOCT4-2A-SOX2 (Homo sapiens)Mammalian Expression Cheng A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214.
MMKMYC-2A-KLF4 (Homo sapiens)Mammalian Expression Cheng A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214.
sgRNA1_NANOGsgRNA1_NANOG (Homo sapiens)Mammalian Expression, Lentiviral, CRISPR Sato CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
sgRNA2_NANOGsgRNA2_NANOG (Homo sapiens)Mammalian Expression, Lentiviral, CRISPR Sato CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
sgRNA3_NANOGsgRNA3_NANOG (Homo sapiens)Mammalian Expression, Lentiviral, CRISPR Sato CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
sgRNA4_NANOGsgRNA4_NANOG (Homo sapiens)Mammalian Expression, Lentiviral, CRISPR Sato CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
pRZ-mNanog-YNLmurine Nanog promoter and YNL (Mus musculus)Lentiviral, Luciferase Okada Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 10.1073/pnas.1418468112. Epub 2015 Mar 23.
pRZ-mOct4-CNLmurine Oct4 promoter and CNL (Mus musculus)Lentiviral, Luciferase Okada Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 10.1073/pnas.1418468112. Epub 2015 Mar 23.
pGF-mSox2-ONLmurine Sox2 promoter and ONL (Mus musculus)Lentiviral, Luciferase Okada Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 10.1073/pnas.1418468112. Epub 2015 Mar 23.
pCX-Klf4Klf4 (Mus musculus)Mammalian Expression Cohen Pluripotent TFs CRE-seq (unpublished)
Oct4-Cr1gRNA for Crispr targeting the OCT4 stop codon (Homo sapiens)Mammalian Expression Huangfu A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells. Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 10.1016/j.stemcr.2015.04.016.
6xO/S lucminimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer (Mus musculus)Mammalian Expression, Luciferase Dailey luciferase reporter plasmid containing 6 tandem copies of the composite Oct4/Sox2 binding site of the FGF4 enhancer. Used for detection of active Oct4/Sox2 complexes (unpublished)
GG-EBNA-OCT45 concatenated gRNA transcriptional cassettes targeting OCT4CRISPR Otonkoski Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001.
pPB-CAG-Klf4-pA-pgk-hphKlf4 (Mus musculus)Mammalian Expression Silva MBD3/NuRD facilitates induction of pluripotency in a context-dependent manner. Cell Stem Cell. 2014 Jul 3;15(1):102-10. doi: 10.1016/j.stem.2014.04.019. Epub 2014 May 15.
pPB-CAG-cNanog-pgk-hphNanog (Gallus gallus)Mammalian Expression Silva Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
pPB-CAG-mNanogHD-pgk-hphNanog (Mus musculus)Mammalian Expression Silva Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
pPB-CAG-zNanogHD-pgk-hphNanog (Danio rerio)Mammalian Expression Silva Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
pPB-CAG-Flag3-pG-mNanog-pgk-hphNanog (Mus musculus)Mammalian Expression Silva Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
pPB-CAG-Flag3-pG-cNanog-pgk-hphNanog (Gallus gallus)Mammalian Expression Silva Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
pPB-CAG-mNanog-mut-pgk-hphNanog (Mus musculus)Mammalian Expression Silva Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
pPB-CAG-Nanog-pA-pgk-hphNanog (Mus musculus)Mammalian Expression Silva Nanog overcomes reprogramming barriers and induces pluripotency in minimal conditions. Curr Biol. 2011 Jan 11;21(1):65-71. doi: 10.1016/j.cub.2010.11.074. Epub 2010 Dec 30.
pPB-CAG-rNanog-pgk-hphNanog (Rattus norvegicus)Mammalian Expression Silva Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeoKlf2 (Mus musculus), Nanog (Mus musculus)Mammalian Expression Silva MBD3/NuRD facilitates induction of pluripotency in a context-dependent manner. Cell Stem Cell. 2014 Jul 3;15(1):102-10. doi: 10.1016/j.stem.2014.04.019. Epub 2014 May 15.
PCXLE-hUL + mTAGBFP2LMyc/Lin28 (Homo sapiens)Mammalian Expression Zovein Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming. Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 10.1186/s13287-017-0581-7.
pCXLE-hS + eGFPSox2 (Homo sapiens)Mammalian Expression Zovein Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming. Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 10.1186/s13287-017-0581-7.
pCXLE-hK + E2-CrimsonKlf4 (Homo sapiens)Mammalian Expression Zovein Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming. Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 10.1186/s13287-017-0581-7.
pCXLE-hOCT3/4-shp53-F + mCherry-2A-puroOct3/4 (Homo sapiens)Mammalian Expression Zovein Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming. Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 10.1186/s13287-017-0581-7.
pCAG-SpCas9-GFP-U6-gRNASpCas9Mammalian Expression, CRISPR Zou pCAG-SpCas9-U6-gRNA (unpublished)
FUGW-d2GFP-200d2GFP + miR-200 family targetsLentiviral Rosen The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states. BMC Biol. 2016 Jun 17;14(1):47. doi: 10.1186/s12915-016-0269-y.
PB-TA-ERNMammalian Expression ; piggyBac transposon Woltjen Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
PB-TAC-ERNMammalian Expression ; piggyBac transposon Woltjen Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
PB-TAG-ERNMammalian Expression ; piggyBac transposon Woltjen Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
PB-TA-ERP2Mammalian Expression ; piggyBac transposon Woltjen Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
PB-TAC-ERP2Mammalian Expression ; piggyBac transposon Woltjen Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
PB-TAG-ERP2Mammalian Expression ; piggyBac transposon Woltjen Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
PB-TAC-OKMSOKMS (Mus musculus)Mammalian Expression ; piggyBac transposon Woltjen KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
PB-TAC-OSKMOSKM (Mus musculus)Mammalian Expression ; piggyBac transposon Woltjen KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
PB-TAC-OK+9MSOK+9MS (Mus musculus)Mammalian Expression ; piggyBac transposon Woltjen KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
PB-TAC-MKOSMKOS (Mus musculus)Mammalian Expression ; piggyBac transposon Woltjen KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
PB-TAC-EB-C5lin-28 homolog A (C. elegans) (Mus musculus)Mammalian Expression ; piggyBac transposon Woltjen KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
PB-TAC-WTSIWTSI (Mus musculus)Mammalian Expression ; piggyBac transposon Woltjen KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
pDONR223_NANOG_WTNANOG (Homo sapiens)Gateway Entry vector Boehm Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
pDONR223_SOX2_WTSOX2 (Homo sapiens)Gateway Entry vector Boehm Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
pREPROII-attBOct4 (Mus musculus), cMyc (Mus musculus), Sox2 (Mus musculus), Kruppel-like factor 4 (Mus musculus)Mammalian Expression, Cre/Lox Kuehn Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector. PLoS One. 2013;8(1):e55170. doi: 10.1371/journal.pone.0055170. Epub 2013 Jan 31.
pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-PuroSOX2-T2A-2xNLS-TdTomato-F2A-Puro (Homo sapiens)CRISPR Otonkoski Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9. Stem Cell Res. 2017 Jul;22:16-19. doi: 10.1016/j.scr.2017.05.005. Epub 2017 May 17.
pUC19-OCT4-T2A-NLS-EmGFP-P2A-PuroOCT4-T2A-NLS-EmGFP-P2A-Puro (Homo sapiens)CRISPR Otonkoski Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9. Stem Cell Res. 2017 Aug;23:105-108. doi: 10.1016/j.scr.2017.07.006. Epub 2017 Jul 11.
pLenti_CamKIIa_GFPGreen fluorescent proteinLentiviral Dolmetsch SHANK3 and IGF1 restore synaptic deficits in neurons from 22q13 deletion syndrome patients. Nature. 2013 Nov 14;503(7475):267-71. doi: 10.1038/nature12618. Epub 2013 Oct 16.
pLenti_CamKIIa_mKate2far-red fluorescent proteinLentiviral Dolmetsch SHANK3 and IGF1 restore synaptic deficits in neurons from 22q13 deletion syndrome patients. Nature. 2013 Nov 14;503(7475):267-71. doi: 10.1038/nature12618. Epub 2013 Oct 16.
pSimple-miR302/367miR302/367 (miRNA cluster) (Homo sapiens)Mammalian Expression Thomson Simultaneous Reprogramming and Gene Correction of Patient Fibroblasts. Stem Cell Reports. 2015 Dec 8;5(6):1109-18. doi: 10.1016/j.stemcr.2015.10.009. Epub 2015 Nov 12.
pSN16vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN17vexGFP-P2A-OCT4 fused to full length HP1 alpha (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN20vexGFP-P2A-OCT4 fused to a 'medium' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN21vexGFP-P2A-OCT4 fused to a 'weak' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN22mCitrine-P2A-SOX2 fused to a 'strong' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN23mCitrine-P2A-SOX2 fused to a 'medium' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN24mCitrine-P2A-SOX2 fused to a 'weak' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN25mCitrine-P2A-SOX2 fused to full length HP1 alpha (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN26mCerulean-P2A-cMYC fused to a 'strong' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN27mCerulean-P2A-cMYC fused to a 'medium' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN28mCerulean-P2A-cMYC fused to a 'weak' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN29mCerulean-P2A-cMYC fused to full length HP1 alpha (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN30mCherry-P2A-KLF4 fused to a 'strong' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN31mCherry-P2A-KLF4 fused to a 'medium' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN32mCherry-P2A-KLF4 fused to a 'weak' VP16 domain (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN33mCherry-P2A-KLF4 fused to full length HP1 alpha (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN34vexGFP-P2A-OCT4 fused to 2 VP16 domains (413-460) separated by linkers (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN35vexGFP-P2A-OCT4 fused to an Engrailed domain (168-281) separated by a linker (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN36vexGFP-P2A-OCT4 fused to 1 VP16 domain (413-460) separated by a linker (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN61vexGFP-P2A-putative OCT4 DBD (138-289) with a T7 tag (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN65mCitrine-P2A-putative SOX2 DBD (41-109) with a V5 tag (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN67mCherry-P2A-putative KLF4 DBD with a 6xHis tag (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
pSN69mCerulean-P2A-putative cMYC DBD with a HA tag (Homo sapiens)Mammalian Expression, Lentiviral Ptashne OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPREAscl1, Brn2 (Mus musculus)Lentiviral Parmar REST suppression mediates neural conversion of adult human fibroblasts via microRNA-dependent and -independent pathways. EMBO Mol Med. 2017 Aug;9(8):1117-1131. doi: 10.15252/emmm.201607471.
PB-GG-OCT4-1-5-PGK-PuroOCT4-gRNAs-1-5-PGK-puro (Other)Mammalian Expression, CRISPR Otonkoski Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
pUCM-AAVS1-TO-hNGN2Neurogenin 2 (Homo sapiens), mCherry (Synthetic), rtTA3G (Other)CRISPR, TALEN Ward Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons. Curr Protoc Cell Biol. 2018 Jun;79(1):e51. doi: 10.1002/cpcb.51. Epub 2018 May 18.
pUCM-CLYBL-hNILNeurogenin 2 (Homo sapiens), ISL LIM Homeobox 1 (Homo sapiens), LIM Homeobox 3 (Homo sapiens), mCherry (Synthetic), rtTA3G (Other)CRISPR, TALEN Ward Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons. Curr Protoc Cell Biol. 2018 Jun;79(1):e51. doi: 10.1002/cpcb.51. Epub 2018 May 18.
pX-APRT-sg2APRT sgRNA-2Mammalian Expression, CRISPR Woltjen Microhomology-assisted scarless genome editing in human iPSCs. Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
p3-HPRT1-S104R-PdTK-mChHPRT genomic DNA (Homo sapiens), puro-deltaTK; CAG::mCherry Woltjen Microhomology-assisted scarless genome editing in human iPSCs. Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
p3-HPRT1-S104Rf-PdTK-mChHPRT genomic DNA (Homo sapiens), puro-deltaTK; CAG::mCherry Woltjen Microhomology-assisted scarless genome editing in human iPSCs. Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
pbG-APRT-J-u32uni-PdTKmChAPRT (Homo sapiens), puro-deltaTK; CAG::mCherry Woltjen Microhomology-assisted scarless genome editing in human iPSCs. Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
pAAVS1-PdTK-CAG-mCh-[uBglII]AAVS1 puro-deltaTK; CAG-mCherry Woltjen Microhomology-assisted scarless genome editing in human iPSCs. Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
pCAG-eGFP-pAGFPMammalian Expression Woltjen Microhomology-assisted scarless genome editing in human iPSCs. Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
H52-pUCM-hNGN2-invNeurogenin 2 (Homo sapiens), mcherry (Other), rtTA3GMammalian Expression, Cre/Lox, CRISPR, TALEN Ward A comprehensive guide for transcription-factor mediated differentiation of human iPSC into neurons (unpublished)
pMx-puro-MGTMef2c, Tbx5 and Gata4 (Mus musculus)Mammalian Expression, Retroviral Qian Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency and quality of induced cardiac myocyte reprogramming. Circ Res. 2015 Jan 16;116(2):237-44. doi: 10.1161/CIRCRESAHA.116.305547. Epub 2014 Nov 21.
pMx-MGTMef2c, Tbx5 and Gata4 (Mus musculus)Mammalian Expression, Retroviral Qian Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency and quality of induced cardiac myocyte reprogramming. Circ Res. 2015 Jan 16;116(2):237-44. doi: 10.1161/CIRCRESAHA.116.305547. Epub 2014 Nov 21.
pAAVS1-TRE-MA4-GFPMLL-AF4 fusion gene (Homo sapiens)Mammalian Expression Kan Respecifying human iPSC-derived blood cells into highly engraftable hematopoietic stem and progenitor cells with a single factor. Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2180-2185. doi: 10.1073/pnas.1718446115. Epub 2018 Jan 31.
pLV-PGK-SNAP-MD-SOX2SNAP-MD-SOX2 (Synthetic)Lentiviral Suter A role for mitotic bookmarking of SOX2 in pluripotency and differentiation. Genes Dev. 2016 Nov 15;30(22):2538-2550. doi: 10.1101/gad.289256.116. Epub 2016 Dec 5.
pLV-PGK-SNAP-MDMUT-SOX2SNAP-MDMUT-SOX2 (Synthetic)Lentiviral Suter A role for mitotic bookmarking of SOX2 in pluripotency and differentiation. Genes Dev. 2016 Nov 15;30(22):2538-2550. doi: 10.1101/gad.289256.116. Epub 2016 Dec 5.
pKIOct4-Halo-IRES-BsdOCT4-Halo-IRES-Bsd (Synthetic)Mammalian Expression Suter Endogenous fluctuations of OCT4 and SOX2 bias pluripotent cell fate decisions bioRxiv 299073
tetO.Sox9.PuroSox9 (Mus musculus)Mammalian Expression, Lentiviral Ahlenius Rapid and efficient induction of functional astrocytes from human pluripotent stem cells. Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2. Epub 2018 Aug 20.
tetO.Nfia.BlastNfia (Mus musculus)Mammalian Expression, Lentiviral Ahlenius Rapid and efficient induction of functional astrocytes from human pluripotent stem cells. Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2. Epub 2018 Aug 20.
tetO.Nfib.HygroNfib (Mus musculus)Mammalian Expression, Lentiviral Ahlenius Rapid and efficient induction of functional astrocytes from human pluripotent stem cells. Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2. Epub 2018 Aug 20.
pMX-eSOX2NRReSOX2NRR (Mus musculus)Mammalian Expression, Retroviral Jauch Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing. Stem Cell Reports. 2018 Aug 14;11(2):593-606. doi: 10.1016/j.stemcr.2018.07.002. Epub 2018 Aug 2.
pMX-eSOX2AHKeSox2AHK (Mus musculus)Mammalian Expression, Retroviral Jauch Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing. Stem Cell Reports. 2018 Aug 14;11(2):593-606. doi: 10.1016/j.stemcr.2018.07.002. Epub 2018 Aug 2.
pMX-eSOX17FNVeSOX17FNV (Mus musculus)Mammalian Expression, Retroviral Jauch Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing. Stem Cell Reports. 2018 Aug 14;11(2):593-606. doi: 10.1016/j.stemcr.2018.07.002. Epub 2018 Aug 2.
pMX-eSOX17WHCeSOX17WHC (Mus musculus)Mammalian Expression, Retroviral Jauch Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing. Stem Cell Reports. 2018 Aug 14;11(2):593-606. doi: 10.1016/j.stemcr.2018.07.002. Epub 2018 Aug 2.
EF1a_KLF4_P2A_Hygro_BarcodeKLF4 (Homo sapiens)Lentiviral Mali Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout. Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008.
EF1a_SOX2_P2A_Hygro_BarcodeSOX2 (Homo sapiens)Lentiviral Mali Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout. Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008.
CLYBL-TO-hNGN2-BSD-mAppleNGN2 (Homo sapiens)Mammalian Expression Ward Next generation vectors for transcription factor mediated differentiation of iPSCs into cortical and motor neurons (unpublished)
CLYBL-TO-hNIL-BSD-mAppleNGN2, ISL1, LHX3 (Homo sapiens)Mammalian Expression Ward Next generation vectors for transcription factor mediated differentiation of iPSCs into cortical and motor neurons (unpublished)
AICSDP-60: SOX2-mEGFPSOX2 Homology Arms with linker-mEGFP (Homo sapiens)Mammalian Expression, CRISPR ; Donor Template Allen Institute for Cell Science Allen Institute for Cell Science Plasmids (unpublished)
Sox2-t2A-GFPSOX2 (Homo sapiens)Mammalian Expression Mitchell KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control. Genes Dev. 2019 Jun 20. pii: gad.324319.119. doi: 10.1101/gad.324319.119.
Sox2-t2A-mCherrySOX2 (Homo sapiens)Mammalian Expression Mitchell KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control. Genes Dev. 2019 Jun 20. pii: gad.324319.119. doi: 10.1101/gad.324319.119.
Nanog-t2A-mCherryNanog (Mus musculus)Mammalian Expression Mitchell KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control. Genes Dev. 2019 Jun 20. pii: gad.324319.119. doi: 10.1101/gad.324319.119.
FUW-TetO-Gata3Gata3 (Mus musculus)Lentiviral Buganim Extensive Nuclear Reprogramming Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells. Cell Stem Cell. 2015 Nov 5;17(5):543-56. doi: 10.1016/j.stem.2015.08.006. Epub 2015 Sep 24.
FUW-TetO-Tfap2cTfap2c (Mus musculus)Lentiviral Buganim Extensive Nuclear Reprogramming Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells. Cell Stem Cell. 2015 Nov 5;17(5):543-56. doi: 10.1016/j.stem.2015.08.006. Epub 2015 Sep 24.
FUW-TetO-Gata6Gata6 (Mus musculus)Lentiviral Buganim Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
FUW-TetO-Sox17Sox17 (Mus musculus)Lentiviral Buganim Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
FUW-TetO-Cdx2Cdx2 (Mus musculus)Lentiviral Buganim Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
FUW-TetO-Klf5Klf5 (Mus musculus)Lentiviral Buganim Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
FUW-TetO-Sox15Sox15 (Mus musculus)Lentiviral Buganim Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
FUW-TetO-Tead4Tead4 (Mus musculus)Lentiviral Buganim Extensive Nuclear Reprogramming Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells. Cell Stem Cell. 2015 Nov 5;17(5):543-56. doi: 10.1016/j.stem.2015.08.006. Epub 2015 Sep 24.
pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLSNuclear EYFP into Elf5 locus (Mus musculus)Mouse Targeting Buganim Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
pNTKV- Utf1-KI-3'UTR-2A-tdTomato-RBGpAtdTomato into Utf1 locus (Mus musculus)Mouse Targeting Buganim Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpAtagBFP into Esrrb locus (Mus musculus)Mouse Targeting Buganim Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
pLVH-EF1a-GFP-P2A-OCT4human OCT4 (Homo sapiens)Lentiviral Hu The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869.
pLVH-EF1a-GFP-P2A-SOX2human SOX2 (Homo sapiens)Lentiviral Hu The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869.
pLVH-EF1a-GFP-P2A-KLF4human KLF4 (Homo sapiens)Lentiviral Hu The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869.
pCW57-GFP-miR-302clusterhuman miR-302 cluster: MIR302B, MIR302C, MIR302A, MIR302D, MIR367 (Homo sapiens)Mammalian Expression, Lentiviral ; Doxycycline inducible, microRNA expression Bárta Oct4-mediated reprogramming induces embryonic-like microRNA expression signatures in human fibroblasts. Sci Rep. 2019 Oct 31;9(1):15759. doi: 10.1038/s41598-019-52294-3.
Sox2-SE-RGM-eGFP targeting vectorSox2-SE-5arm-RGM-Sox2-SE-3arm (Mus musculus)Mouse Targeting Jaenisch Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs. Mol Cell. 2019 Aug 15. pii: S1097-2765(19)30502-7. doi: 10.1016/j.molcel.2019.06.045.

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