| pWZL Blast myc | c-myc (Homo sapiens) | Mammalian Expression, Retroviral |
Hahn
| Transformation of human and murine fibroblasts without viral oncoproteins.
Mol Cell Biol. 2005 Aug . 25(15):6464-74.
|
| RCAS-myc | c-myc (Homo sapiens) | Retroviral |
Orsulic
| Induction of ovarian cancer by defined multiple genetic changes in a mouse model system.
Cancer Cell. 2002 Feb . 1(1):53-62.
|
| pMXs-Nanog | Nanog homeobox (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Cell. 2006 Aug 25. 126(4):663-76.
|
| pMXs-Oct3/4 | POU domain, class 5, transcription factor 1 (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Cell. 2006 Aug 25. 126(4):663-76.
|
| pMXs-Sox2 | SRY-box containing gene 2 (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Cell. 2006 Aug 25. 126(4):663-76.
|
| pMXs-Klf4 | Kruppel-like factor 4 (gut) (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Cell. 2006 Aug 25. 126(4):663-76.
|
| pMXs-c-Myc | myelocytomatosis oncogene (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Cell. 2006 Aug 25. 126(4):663-76.
|
| pCAG-HA-Sox2-IP | Sox2 (Mus musculus) | Mammalian Expression |
Yamanaka
| Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells.
J Biol Chem. 2005 Jul 1. 280(26):24371-9.
|
| pCAG-Myc-Oct3/4-IP | Oct3/4 (Mus musculus) | Mammalian Expression |
Yamanaka
| Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells.
J Biol Chem. 2005 Jul 1. 280(26):24371-9.
|
| pCAG-Oct3/4-IRES-Neo | Oct3/4 (Mus musculus) | Mammalian Expression |
Yamanaka
| Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development.
Mol Cell Biol. 2003 Apr . 23(8):2699-708.
|
| pCAG-Sox2-IRES-Neo | Sox2 (Mus musculus) | Mammalian Expression |
Yamanaka
| Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development.
Mol Cell Biol. 2003 Apr . 23(8):2699-708.
|
| pTV-Nanog Hyg | IRES-Hyg-pA cassette flanked by Nanog homologous regions (Mus musculus) | Mammalian Expression ; Beta-gal |
Yamanaka
| The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells.
Cell. 2003 May 30. 113(5):631-42.
|
| pTV-Nanog Bgeo | IRES-Bgeo-pA cassette flanked by Nanog homologous regions (Mus musculus) | Mammalian Expression ; Beta-gal |
Yamanaka
| The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells.
Cell. 2003 May 30. 113(5):631-42.
|
| pPyCAG-Nanog-IP | Nanog (Mus musculus) | Mammalian Expression |
Yamanaka
| The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells.
Cell. 2003 May 30. 113(5):631-42.
|
| pDEST17-Nanog | Nanog (Mus musculus) | Bacterial Expression |
Yamanaka
| The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells.
Cell. 2003 May 30. 113(5):631-42.
|
| pBS human c-Myc | c-Myc (Homo sapiens) | Bacterial Expression |
Massague
| Mad upregulation and Id2 repression accompany transforming growth factor (TGF)-beta-mediated epithelial cell growth suppression.
J Biol Chem. 2003 Sep 12. 278(37):35444-50.
|
| pBS mouse c-Myc | c-Myc (Mus musculus) | Bacterial Expression |
Massague
| Mad upregulation and Id2 repression accompany transforming growth factor (TGF)-beta-mediated epithelial cell growth suppression.
J Biol Chem. 2003 Sep 12. 278(37):35444-50.
|
| pMXs-Oct3/4-IP | POU domain, class 5, transcription factor 1 (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Cell. 2006 Aug 25. 126(4):663-76.
|
| pMXs-Sox2-IP | SRY-box containing gene 2 (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Cell. 2006 Aug 25. 126(4):663-76.
|
| pMXs-Klf4-IP | Kruppel-like factor 4 (gut) (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Cell. 2006 Aug 25. 126(4):663-76.
|
| pMXs-c-Myc-IP | myelocytomatosis oncogene (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Cell. 2006 Aug 25. 126(4):663-76.
|
| pLOVE-Klf4 | Kruppel-like factor 4 (Mus musculus) | Mammalian Expression, Lentiviral |
Ramalho-Santos
| Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection.
Cell Stem Cell. 2007 Sep 13. ():.
|
| pLOVE-Nmyc | v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mus musculus) | Mammalian Expression, Lentiviral |
Ramalho-Santos
| Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection.
Cell Stem Cell. 2007 Sep 13. ():.
|
| pLOVE-Sox2 | SRY (sex determining region Y)-box 2 (Mus musculus) | Mammalian Expression, Lentiviral |
Ramalho-Santos
| Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection.
Cell Stem Cell. 2007 Sep 13. ():.
|
| pcDNA3-cmyc | cmyc (Homo sapiens) | Mammalian Expression |
El-Deiry
| Direct repression of FLIP expression by c-myc is a major determinant of TRAIL sensitivity.
Mol Cell Biol. 2004 Oct . 24(19):8541-55.
|
| pGEM-LIN28 | Homo sapiens lin-28 homolog (C. elegans) (Homo sapiens) | Bacterial Expression |
Thomson
| Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells.
Science. 2007 Nov 20. ():.
|
| pGEM-Nanog | Homo sapiens Nanog homeobox (Homo sapiens) | Bacterial Expression |
Thomson
| Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells.
Science. 2007 Nov 20. ():.
|
| pGEM-Oct4 | Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (Homo sapiens) | Bacterial Expression |
Thomson
| Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells.
Science. 2007 Nov 20. ():.
|
| pGEM-Sox2 | Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (Homo sapiens) | Bacterial Expression |
Thomson
| Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells.
Science. 2007 Nov 20. ():.
|
| deltaMyc-HA | c-MYC (Homo sapiens) | Mammalian Expression |
Vogelstein
| Identification of CDK4 as a target of c-MYC.
Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34.
|
| pSin-EF2-Sox2-Pur | Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (Homo sapiens) | Mammalian Expression, Lentiviral |
Thomson
| Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells.
Science. 2007 Nov 20. ():.
|
| pSin-EF2-Nanog-Pur | Homo sapiens Nanog homeobox (Homo sapiens) | Mammalian Expression, Lentiviral |
Thomson
| Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells.
Science. 2007 Nov 20. ():.
|
| pSin-EF2-Oct4-Pur | Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (Homo sapiens) | Mammalian Expression, Lentiviral |
Thomson
| Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells.
Science. 2007 Nov 20. ():.
|
| pSin-EF2-LIN28-Pur | Homo sapiens lin-28 homolog (C. elegans) (Homo sapiens) | Mammalian Expression, Lentiviral |
Thomson
| Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells.
Science. 2007 Nov 20. ():.
|
| pMXs-hOCT3/4 | OCT3/4 (Homo sapiens) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
Cell. 2007 Nov 30. 131(5):861-72.
|
| pMXs-hSOX2 | SOX2 (Homo sapiens) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
Cell. 2007 Nov 30. 131(5):861-72.
|
| pMXs-hKLF4 | KLF4 (Homo sapiens) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
Cell. 2007 Nov 30. 131(5):861-72.
|
| pMXs-hc-MYC | c-MYC (Homo sapiens) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
Cell. 2007 Nov 30. 131(5):861-72.
|
| pMIG-hOCT4 | POU class 5 homeobox 1 (Homo sapiens) | Mammalian Expression, Retroviral |
Daley
| Reprogramming of human somatic cells to pluripotency with defined factors.
Nature. 2008 Jan 10. 451(7175):141-6.
|
| pMIG-hSOX2 | SRY (sex determining region Y)-box 2 (Homo sapiens) | Mammalian Expression, Retroviral |
Daley
| Reprogramming of human somatic cells to pluripotency with defined factors.
Nature. 2008 Jan 10. 451(7175):141-6.
|
| pMIG-hKLF4 | Kruppel-like factor 4 (gut) (Homo sapiens) | Mammalian Expression, Retroviral |
Daley
| Reprogramming of human somatic cells to pluripotency with defined factors.
Nature. 2008 Jan 10. 451(7175):141-6.
|
| pBabe-c-myc-zeo | c-myc (Homo sapiens) | Mammalian Expression, Retroviral |
Lindquist
| Heat shock factor 1 is a powerful multifaceted modifier of carcinogenesis.
Cell. 2007 Sep 21. 130(6):1005-18.
|
| pMXs-hOCT4 (Plath) | POU domain, class 5, transcription factor 1 (Homo sapiens) | Mammalian Expression, Retroviral |
Plath
| Generation of human induced pluripotent stem cells from dermal fibroblasts.
Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8.
|
| pMXs-hSOX2 (Plath) | SRY (sex determining region Y)-box 2 (Homo sapiens) | Mammalian Expression, Retroviral |
Plath
| Generation of human induced pluripotent stem cells from dermal fibroblasts.
Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8.
|
| pMXs-hcMYC (Plath) | v-myc myelocytomatosis viral oncogene homolog (avian) (Homo sapiens) | Mammalian Expression, Retroviral |
Plath
| Generation of human induced pluripotent stem cells from dermal fibroblasts.
Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8.
|
| pMXs-hKLF4 (Plath) | Kruppel-like factor 4 (gut) (Homo sapiens) | Mammalian Expression, Retroviral |
Plath
| Generation of human induced pluripotent stem cells from dermal fibroblasts.
Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8.
|
| pMXs-hNANOG (Plath) | Nanog homeobox (Homo sapiens) | Mammalian Expression, Retroviral |
Plath
| Generation of human induced pluripotent stem cells from dermal fibroblasts.
Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8.
|
| MSCV h c-MYC IRES GFP | c-MYC (Homo sapiens) | Retroviral |
Cleveland
|
Cleveland lab plasmids
(unpublished)
|
| tetO7_Ef1a_OCT4 | OCT4 (Homo sapiens) | Mammalian Expression, Lentiviral, Cre/Lox |
Cheng
| Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts.
Stem Cells. 2008 Aug . 26(8):1998-2005.
|
| tetO7_Ef1a_SOX2 | SOX2 (Homo sapiens) | Mammalian Expression, Lentiviral, Cre/Lox |
Cheng
| Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts.
Stem Cells. 2008 Aug . 26(8):1998-2005.
|
| Ef1a_NANOG_Ires_Puro | NANOG (Homo sapiens) | Mammalian Expression, Lentiviral, Cre/Lox |
Cheng
| Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts.
Stem Cells. 2008 Aug . 26(8):1998-2005.
|
| Ef1a_LIN28_Ires_Puro | LIN28 (Homo sapiens) | Mammalian Expression, Lentiviral, Cre/Lox |
Cheng
| Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts.
Stem Cells. 2008 Aug . 26(8):1998-2005.
|
| pBabepuro-myc-ER | c-myc (Homo sapiens) | Mammalian Expression, Retroviral |
El-Deiry
| Direct repression of FLIP expression by c-myc is a major determinant of TRAIL sensitivity.
Mol Cell Biol. 2004 Oct . 24(19):8541-55.
|
| pLV-tetO-myc T58A | c-myc (Homo sapiens) | Mammalian Expression, Lentiviral |
Hochedlinger
| Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse.
Cell Stem Cell. 2008 Mar 6. 2(3):230-40.
|
| pLV-tetO-Klf4 | Klf4 (Mus musculus) | Mammalian Expression, Lentiviral |
Hochedlinger
| Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse.
Cell Stem Cell. 2008 Mar 6. 2(3):230-40.
|
| pLV-tetO-Sox2 | Sox2 (Mus musculus) | Mammalian Expression, Lentiviral |
Hochedlinger
| Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse.
Cell Stem Cell. 2008 Mar 6. 2(3):230-40.
|
| pLV-tetO-Oct4 | Oct4 (Mus musculus) | Mammalian Expression, Lentiviral |
Hochedlinger
| Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse.
Cell Stem Cell. 2008 Mar 6. 2(3):230-40.
|
| pAd-Sox2 | Sox2 (Mus musculus) | Mammalian Expression, Adenoviral |
Hochedlinger
| Induced Pluripotent Stem Cells Generated Without Viral Integration.
Science. 2008 Sep 25. ():.
|
| pAd-Oct4 | Oct4 (Mus musculus) | Mammalian Expression, Adenoviral |
Hochedlinger
| Induced Pluripotent Stem Cells Generated Without Viral Integration.
Science. 2008 Sep 25. ():.
|
| pAd-cMyc T58A | c-myc (Homo sapiens) | Mammalian Expression, Adenoviral |
Hochedlinger
| Induced Pluripotent Stem Cells Generated Without Viral Integration.
Science. 2008 Sep 25. ():.
|
| pAd-Klf4 | Klf4 (Mus musculus) | Mammalian Expression, Adenoviral |
Hochedlinger
| Induced Pluripotent Stem Cells Generated Without Viral Integration.
Science. 2008 Sep 25. ():.
|
| pCX-OKS-2A | Oct3/4-2A-Klf4-2A-Sox2 (Mus musculus) | Mammalian Expression |
Yamanaka
| Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors.
Science. 2008 Oct 9. ():.
|
| pCX-cMyc | c-Myc (Mus musculus) | Mammalian Expression |
Yamanaka
| Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors.
Science. 2008 Oct 9. ():.
|
| FU-tet-o-hc-myc | c-myc (Homo sapiens) | Mammalian Expression, Lentiviral |
Hochedlinger
| A high-efficiency system for the generation and study of human induced pluripotent stem cells.
Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
|
| FU-tet-o-hKLF4 | Klf4 (Homo sapiens) | Mammalian Expression, Lentiviral |
Hochedlinger
| A high-efficiency system for the generation and study of human induced pluripotent stem cells.
Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
|
| FU-tet-o-hOct4 | Oct4 (Homo sapiens) | Mammalian Expression, Lentiviral |
Hochedlinger
| A high-efficiency system for the generation and study of human induced pluripotent stem cells.
Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
|
| FU-tet-o-hSox2 | Sox2 (Homo sapiens) | Mammalian Expression, Lentiviral |
Hochedlinger
| A high-efficiency system for the generation and study of human induced pluripotent stem cells.
Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
|
| pMSCV-Flag-cMyc T58A | cMyc (Homo sapiens) | Mammalian Expression, Retroviral |
Belmonte
| Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes.
Nat Biotechnol. 2008 Nov . 26(11):1276-84.
|
| pMSCVpuro-Flag-cMyc T58A | cMyc (Homo sapiens) | Mammalian Expression, Retroviral |
Belmonte
| Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes.
Nat Biotechnol. 2008 Nov . 26(11):1276-84.
|
| TetO-FUW-OSKM | Oct-4 (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Reprogramming of murine and human somatic cells using a single polycistronic vector.
Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
|
| TetO-FUW-Klf4 | Klf4 (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells.
Cell Stem Cell. 2008 Feb 7. 2(2):151-9.
|
| TetO-FUW-oct4 | oct4 (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells.
Cell Stem Cell. 2008 Feb 7. 2(2):151-9.
|
| TetO-FUW-cMYC | cMYC (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells.
Cell Stem Cell. 2008 Feb 7. 2(2):151-9.
|
| FUW-SOKM | Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Reprogramming of murine and human somatic cells using a single polycistronic vector.
Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
|
| TetO-FUW-sox2 | sox2 (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells.
Cell Stem Cell. 2008 Feb 7. 2(2):151-9.
|
| FUW-SOK | Sox2-F2A-Oct4-T2A-Klf4 (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Reprogramming of murine and human somatic cells using a single polycistronic vector.
Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
|
| FUW-OSKM | Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Reprogramming of murine and human somatic cells using a single polycistronic vector.
Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
|
| FUW-SO | Sox2-F2A-Oct4 (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Reprogramming of murine and human somatic cells using a single polycistronic vector.
Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
|
| FUW-SM | Sox2-F2A-cMyc (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Reprogramming of murine and human somatic cells using a single polycistronic vector.
Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
|
| FUW-KO | Klf4-F2A-Oct4 (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Reprogramming of murine and human somatic cells using a single polycistronic vector.
Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
|
| FUW-KM | Klf4-F2A-cMyc (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Reprogramming of murine and human somatic cells using a single polycistronic vector.
Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
|
| FUW-tetO-hMYC | c-MYC (Homo sapiens) | Mammalian Expression, Lentiviral |
Jaenisch
| A drug-inducible system for direct reprogramming of human somatic cells to pluripotency.
Cell Stem Cell. 2008 Sep 11. 3(3):346-53.
|
| FUW-tetO-hSOX2 | SOX2 (Homo sapiens) | Mammalian Expression, Lentiviral |
Jaenisch
| A drug-inducible system for direct reprogramming of human somatic cells to pluripotency.
Cell Stem Cell. 2008 Sep 11. 3(3):346-53.
|
| FUW-tetO-hKLF4 | KLF4 (Homo sapiens) | Mammalian Expression, Lentiviral |
Jaenisch
| A drug-inducible system for direct reprogramming of human somatic cells to pluripotency.
Cell Stem Cell. 2008 Sep 11. 3(3):346-53.
|
| FUW-tetO-hOCT4 | OCT4 (Homo sapiens) | Mammalian Expression, Lentiviral |
Jaenisch
| A drug-inducible system for direct reprogramming of human somatic cells to pluripotency.
Cell Stem Cell. 2008 Sep 11. 3(3):346-53.
|
| FUW-tetO-lox-hKLF4 | KLF4 (Homo sapiens) | Mammalian Expression, Lentiviral |
Jaenisch
| Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors
Cell, Volume 136, Issue 5, 964-977, 6 March 2009
|
| FUW-tetO-lox-hOCT4 | OCT4 (Homo sapiens) | Mammalian Expression, Lentiviral |
Jaenisch
| Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors
Cell, Volume 136, Issue 5, 964-977, 6 March 2009
|
| FUW-tetO-lox-hSOX2 | SOX2 (Homo sapiens) | Mammalian Expression, Lentiviral |
Jaenisch
| Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors
Cell, Volume 136, Issue 5, 964-977, 6 March 2009
|
| pCAGMKOSiE | myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Mus musculus) | Mammalian Expression |
Kaji
| Virus-free induction of pluripotency and subsequent excision of reprogramming factors.
Nature. 2009 Mar 1. ():.
|
| pCAG2LMKOSimO | c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2-ires-mOrange (Mus musculus) | Mammalian Expression |
Kaji
| Virus-free induction of pluripotency and subsequent excision of reprogramming factors.
Nature. 2009 Mar 1. ():.
|
| PB-TET-mcMyc | cMyc (Mus musculus) | Mammalian Expression ; piggyBac |
Nagy
| piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.
Nature. 2009 Mar 1. ():.
|
| PB-TET-mKLF4 | KLF4 (Mus musculus) | Mammalian Expression ; piggyBac |
Nagy
| piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.
Nature. 2009 Mar 1. ():.
|
| PB-TET-mOct4 | Oct4 (Mus musculus) | Mammalian Expression ; piggyBac |
Nagy
| piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.
Nature. 2009 Mar 1. ():.
|
| PB-TET-mSox2 | Sox2 (Mus musculus) | Mammalian Expression ; piggyBac |
Nagy
| piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.
Nature. 2009 Mar 1. ():.
|
| pEP4 E02S EN2L | Oct4 and Sox2; Nanog and Lin28 (Homo sapiens) | Episomal/EBNA |
Thomson
| Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences
Science. 2009 Mar 26.
|
| pEP4 E02S CK2M EN2L | Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (Homo sapiens) | Episomal/EBNA |
Thomson
| Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences
Science. 2009 Mar 26.
|
| pEP4 E02S EN2K | Oct4 and Sox2; Nanog and Klf4 (Homo sapiens) | Episomal/EBNA |
Thomson
| Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences
Science. 2009 Mar 26.
|
| pCEP4-M2L | MYC (Homo sapiens), LIN28 (Homo sapiens) | Episomal/EBNA |
Thomson
| Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences
Science. 2009 Mar 26.
|
| pEP4 E02S ET2K | Oct4 and Sox2; SV40LT and Klf4 (Homo sapiens) | Episomal/EBNA |
Thomson
| Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences
Science. 2009 Mar 26.
|
| PB-TET-MKOS | cMyc, KLF4, Oct4, Sox2 (Mus musculus) | Mammalian Expression ; piggyBac |
Nagy
| piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.
Nature. 2009 Mar 1. ():.
|
| pBABE-puro-mouse Oct4 | Oct4 (Mus musculus) | Mammalian Expression, Retroviral |
Tantin
| A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress.
Genes Dev. 2009 Jan 15. 23(2):208-22.
|
| pSIN4-EF2-O2S | Oct4, Sox2 (Homo sapiens) | Lentiviral ; reprogramming |
Thomson
| Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences
Science. 2009 Mar 26.
|
| pSIN4-EF2-N2L | Nanog, Lin28 (Homo sapiens) | Mammalian Expression, Lentiviral ; reprogramming |
Thomson
| Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences
Science. 2009 Mar 26.
|
| pSIN4-CMV-K2M | Klf4, cMyc (Homo sapiens) | Mammalian Expression, Lentiviral ; reprogramming |
Thomson
| Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences
Science. 2009 Mar 26.
|
| Oct4-ires-EGFP(lox neo) | EGFP, Oct4 (Mus musculus) | Mouse Targeting |
Jaenisch
| Oct4 expression is not required for mouse somatic stem cell self-renewal
Cell Stem Cell. 2007 Oct 11;1(4):403-15.
|
| pLM-vexGFP-Oct4 | vexGFP_2A_OCT4 (Homo sapiens) | Lentiviral |
Sadelain
| Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation.
Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
|
| pLM-mCitrine-Sox2 | mCitrine_2A_SOX2 (Homo sapiens) | Lentiviral |
Sadelain
| Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation.
Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
|
| pLM-mCherry-Klf4 | mCherry_2A_KLF4 (Homo sapiens) | Lentiviral |
Sadelain
| Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation.
Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
|
| pLM-mCerulean-cMyc | mCerulean-2A-MYC (Homo sapiens) | Lentiviral |
Sadelain
| Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation.
Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
|
| OKSIM | Oct4 KLF4 Sox2 c-Myc (Homo sapiens) | Mammalian Expression, Lentiviral, Cre/Lox |
Cibelli
| Human Induced Pluripotent Stem Cells Produced Under Xeno-Free Conditions.
Stem Cells Dev. 2009 Dec 23. ():.
|
| HUW-TetO-hNanog | Nanog (Homo sapiens) | Mammalian Expression, Lentiviral |
Jaenisch
| Direct cell reprogramming is a stochastic process amenable to acceleration.
Nature. 2009 Dec 3. 462(7273):595-601.
|
| pMXs-Hu-L-Myc | L-Myc (Homo sapiens) | Mammalian Expression, Retroviral |
Yamanaka
| Promotion of direct reprogramming by transformation-deficient Myc.
Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
|
| pMXs-Ms-L-Myc | L-Myc (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Promotion of direct reprogramming by transformation-deficient Myc.
Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
|
| pMXs-Ms-c-Myc-W136E | c-Myc (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Promotion of direct reprogramming by transformation-deficient Myc.
Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
|
| pBRPyCAG-fmKlf4-DsRed-Ip | Klf4 (Mus musculus) | Mammalian Expression, Cre/Lox |
Jaenisch
| Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs.
Proc Natl Acad Sci U S A. 2010 May 18. 107(20):9222-7.
|
| pWZL Sox2 | Sox2 (Homo sapiens) | Mammalian Expression, Retroviral |
Meyerson
| SOX2 is an amplified lineage-survival oncogene in lung and esophageal squamous cell carcinomas.
Nat Genet. 2009 Nov . 41(11):1238-42.
|
| pWZL Sox2 R74P | Sox2 (Homo sapiens) | Mammalian Expression, Retroviral |
Meyerson
| SOX2 is an amplified lineage-survival oncogene in lung and esophageal squamous cell carcinomas.
Nat Genet. 2009 Nov . 41(11):1238-42.
|
| pMSCV-mLin28A | lin-28 homolog A (Mus musculus) | Mammalian Expression, Retroviral |
Daley
| Lin28 promotes transformation and is associated with advanced human malignancies.
Nat Genet. 2009 Jul . 41(7):843-8.
|
| pBABE-hLin28B | lin-28 homolog B (Homo sapiens) | Mammalian Expression, Retroviral |
Daley
| Lin28 promotes transformation and is associated with advanced human malignancies.
Nat Genet. 2009 Jul . 41(7):843-8.
|
| pcDNA3.3_KLF4 | 5'UTR-KLF4-3'UTR (Homo sapiens) | Mammalian Expression |
Rossi
| Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA.
Cell Stem Cell. 2010 Sep 29. ():.
|
| pcDNA3.3_OCT4 | 5'UTR-OCT4-3'UTR (Homo sapiens) | Mammalian Expression |
Rossi
| Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA.
Cell Stem Cell. 2010 Sep 29. ():.
|
| pcDNA3.3_SOX2 | 5'UTR-SOX2-3'UTR (Homo sapiens) | Mammalian Expression |
Rossi
| Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA.
Cell Stem Cell. 2010 Sep 29. ():.
|
| pcDNA3.3_c-MYC | 5'UTR-c-MYC-3'UTR (Homo sapiens) | Mammalian Expression |
Rossi
| Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA.
Cell Stem Cell. 2010 Sep 29. ():.
|
| pCXLE-hOCT3/4 | OCT3/4 (Homo sapiens) | Mammalian Expression |
Yamanaka
| A more efficient method to generate integration-free human iPS cells.
Nat Methods. 2011 Apr 3. ():.
|
| pCXLE-hOCT3/4-shp53-F | OCT3/4 (Homo sapiens) | Mammalian Expression |
Yamanaka
| A more efficient method to generate integration-free human iPS cells.
Nat Methods. 2011 Apr 3. ():.
|
| pCXLE-hSK | SOX2, KLF4 (Homo sapiens) | Mammalian Expression |
Yamanaka
| A more efficient method to generate integration-free human iPS cells.
Nat Methods. 2011 Apr 3. ():.
|
| pCXLE-hMLN | C-MYC, LIN28, NANOG (Homo sapiens) | Mammalian Expression |
Yamanaka
| A more efficient method to generate integration-free human iPS cells.
Nat Methods. 2011 Apr 3. ():.
|
| pCXLE-hUL | L-MYC (Homo sapiens) | Mammalian Expression |
Yamanaka
| A more efficient method to generate integration-free human iPS cells.
Nat Methods. 2011 Apr 3. ():.
|
| pEB-C5 | Oct4, Sox2, Klf4, c-Myc, Lin28 (Mus musculus) | Mammalian Expression |
Cheng
| Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures.
Cell Res. 2011 Jan 18. ():.
|
| pCDNA3.1-Nanog | Nanog (Homo sapiens) | Mammalian Expression |
Cheng
| Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures.
Cell Res. 2011 Jan 18. ():.
|
| pMXs-GLIS1 | GLIS1 (Homo sapiens) | Mammalian Expression, Retroviral |
Yamanaka
| Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1.
Nature. 2011 . 474(7350):225-9.
|
| OCT4-eGFP-PGK-Puro | OCT4 (Homo sapiens) | targeting donor |
Jaenisch
| Genetic engineering of human pluripotent cells using TALE nucleases.
Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
|
| OCT4-2A-eGFP-PGK-Puro | OCT4 (Homo sapiens) | targeting donor |
Jaenisch
| Genetic engineering of human pluripotent cells using TALE nucleases.
Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
|
| OCT4-eGFP-2A-Puro | OCT4 (Homo sapiens), eGFP | targeting donor |
Jaenisch
| Genetic engineering of human pluripotent cells using TALE nucleases.
Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927.
|
| pMXs-mGLIS1 | GLIS1, Gateway casette A (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1.
Nature. 2011 . 474(7350):225-9.
|
| pMXs-GLIS1 (FLJ33315SAAN) | GLIS1, Gateway casette A (Homo sapiens) | Mammalian Expression, Retroviral |
Yamanaka
| Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1.
Nature. 2011 . 474(7350):225-9.
|
| pBpuro - HA KLF4 FL | KLF4 (Homo sapiens) | Mammalian Expression, Retroviral |
Ruppert
| A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context.
Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
|
| pBpuro - HA KLF4 FL delta K5S | KLF4 (Homo sapiens) | Mammalian Expression, Retroviral |
Ruppert
| A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context.
Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
|
| pcDNA3.1 - HA-KLF4 FL | KLF4 (Homo sapiens) | Mammalian Expression |
Ruppert
| A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context.
Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
|
| pcDNA3.1 - HA-KLF4 FL delta K5S | KLF4 (Homo sapiens) | Mammalian Expression |
Ruppert
| A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context.
Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
|
| pLJD-KLF4-ER | KLF4 (Homo sapiens) | Mammalian Expression, Retroviral |
Ruppert
| A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context.
Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
|
| pMIR-Report-Luc-KLF4-FL | KLF4 (Homo sapiens) | Mammalian Expression, Luciferase |
Ruppert
| A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context.
Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
|
| pBpuro_KLF4_ER | KLF4 (Homo sapiens) | Mammalian Expression, Retroviral |
Ruppert
| Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia.
Oncogene. 2005 Feb 24;24(9):1491-500.
|
| pLenti-EF1a-SOX2 | TALE-Sox2 (Synthetic) | Mammalian Expression, Lentiviral |
Zhang
| Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription.
Nat Biotechnol. 2011 Jan 19. ():.
|
| pLenti-EF1a-KLF4 | TALE-Klf4 (Synthetic) | Mammalian Expression, Lentiviral |
Zhang
| Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription.
Nat Biotechnol. 2011 Jan 19. ():.
|
| pLenti-EF1a-OCT4 | TALE-Oct4 (Synthetic) | Mammalian Expression, Lentiviral |
Zhang
| Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription.
Nat Biotechnol. 2011 Jan 19. ():.
|
| pCX4-Oct4 | Oct4 (Homo sapiens) | Retroviral |
Dowdy
| Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells.
Nature. 2012 Jan 25;482(7384):216-20. doi: 10.1038/nature10821.
|
| pCX4-Sox2 | Sox2 (Homo sapiens) | Retroviral |
Dowdy
| Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells.
Nature. 2012 Jan 25;482(7384):216-20. doi: 10.1038/nature10821.
|
| pCX4-KLF4 | kruppel-like factor 4 (Homo sapiens) | Retroviral |
Dowdy
| Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells.
Nature. 2012 Jan 25;482(7384):216-20. doi: 10.1038/nature10821.
|
| pCX4-cMyc | cMyc (Homo sapiens) | Retroviral |
Dowdy
| Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells.
Nature. 2012 Jan 25;482(7384):216-20. doi: 10.1038/nature10821.
|
| pWPXL-c-Myc | c-Myc (Mus musculus) | Mammalian Expression, Lentiviral |
Weinberg
| Slug and Sox9 cooperatively determine the mammary stem cell state.
Cell. 2012 Mar 2;148(5):1015-28.
|
| pWPXL-Klf4 | Klf4 (Mus musculus) | Mammalian Expression, Lentiviral |
Weinberg
| Slug and Sox9 cooperatively determine the mammary stem cell state.
Cell. 2012 Mar 2;148(5):1015-28.
|
| pCXLE-hGLIS1 | human GLIS1 (Homo sapiens) | Mammalian Expression |
Yamanaka
| An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells.
Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
|
| pCEP4_WT_OCT4 | POU5F1 (Homo sapiens) | Mammalian Expression |
Thomson
| Phosphorylation regulates human OCT4.
Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2.
|
| FUW-TetO-Nanog | Nanog (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Single-Cell Expression Analyses during Cellular Reprogramming Reveal an Early Stochastic and a Late Hierarchic Phase.
Cell. 2012 Sep 14;150(6):1209-22. doi: 10.1016/j.cell.2012.08.023.
|
| pCE-hSK | SOX2, KLF4 (Homo sapiens) | Mammalian Expression |
Yamanaka
| An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells.
Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
|
| pCE-hUL | L-MYC, LIN28 (Homo sapiens) | Mammalian Expression |
Yamanaka
| An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells.
Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
|
| pMYC-GFP | c-Myc (Homo sapiens) | Mammalian Expression, Bacterial Expression |
Olopade
| c-Myc activates BRCA1 gene expression through distal promoter elements in breast cancer cells.
BMC Cancer. 2011 Jun 13;11:246. doi: 10.1186/1471-2407-11-246.
|
| pD40-His/V5-c-Myc | c-Myc (Mus musculus) | Mammalian Expression |
Sears
| A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells.
Nat Cell Biol. 2004 Apr;6(4):308-18. Epub 2004 Mar 14.
|
| pMXs-mM₃O-IP | mM₃O (Mus musculus) | Mammalian Expression, Retroviral |
Kikyo
| Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD.
Stem Cells. 2011 Sep;29(9):1349-61. doi: 10.1002/stem.684.
|
| pMXs-hM₃O-IP | hM₃O (Homo sapiens) | Mammalian Expression, Retroviral |
Kikyo
| Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD.
Stem Cells. 2011 Sep;29(9):1349-61. doi: 10.1002/stem.684.
|
| pCDH-puro-cMyc | MYC (Homo sapiens) | Mammalian Expression, Lentiviral |
Wang
| Inhibition of BET bromodomain targets genetically diverse glioblastoma.
Clin Cancer Res. 2013 Apr 1;19(7):1748-59. doi: 10.1158/1078-0432.CCR-12-3066. Epub 2013 Feb 12.
|
| Tet-pLKO-puro-SOX2 | SOX2 shRNA (Synthetic) | Mammalian Expression, Lentiviral, RNAi |
Rudin
| Comprehensive genomic analysis identifies SOX2 as a frequently amplified gene in small-cell lung cancer.
Nat Genet. 2012 Oct;44(10):1111-6. doi: 10.1038/ng.2405. Epub 2012 Sep 2.
|
| Nanog-2A-mCherry | Nanog (Mus musculus) | CRISPR |
Jaenisch
| One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering.
Cell. 2013 Aug 27. pii: S0092-8674(13)01016-7. doi: 10.1016/j.cell.2013.08.022.
|
| Oct4-IRES-eGFP-PGK-Neo | Oct4 (Mus musculus) | CRISPR |
Jaenisch
| One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering.
Cell. 2013 Aug 27. pii: S0092-8674(13)01016-7. doi: 10.1016/j.cell.2013.08.022.
|
| pDNR-hSox2 | Sox2 (Homo sapiens) | Cre/Lox ; donor vector |
McKinnon
|
McKinnon Lab plasmids
(unpublished)
|
| pLPS-hSox2-gfp | Sox2 (Homo sapiens) | Mammalian Expression, Cre/Lox ; acceptor vector |
McKinnon
|
McKinnon Lab plasmids
(unpublished)
|
| pLP-hSox2-ires-gfp | Sox2 (Homo sapiens) | Mammalian Expression, Cre/Lox ; acceptor vector |
McKinnon
|
McKinnon Lab plasmids
(unpublished)
|
| pLP-TRE2-hSox2 | Sox2 (Homo sapiens) | Mammalian Expression, Cre/Lox ; donor vector |
McKinnon
|
McKinnon Lab plasmids
(unpublished)
|
| pMXs-hu-N-Myc | N-myc (Homo sapiens) | Mammalian Expression, Retroviral |
Yamanaka
| Promotion of direct reprogramming by transformation-deficient Myc.
Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
|
| pMXs-hu-c-Myc-dN1 | c-Myc -dN1 (Homo sapiens) | Mammalian Expression, Retroviral |
Yamanaka
| Promotion of direct reprogramming by transformation-deficient Myc.
Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
|
| pMXs-hu-c-Myc-dN2 | c-Myc -dN2 (Homo sapiens) | Mammalian Expression, Retroviral |
Yamanaka
| Promotion of direct reprogramming by transformation-deficient Myc.
Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
|
| pMXs-ms-c-Myc-V394D | c-Myc V394D (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Promotion of direct reprogramming by transformation-deficient Myc.
Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
|
| pMXs-ms-c-Myc-L420P | c-Myc L420P (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Promotion of direct reprogramming by transformation-deficient Myc.
Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
|
| pMXs-ms-L-Myc-L351P | L-Myc-L351P (Mus musculus) | Mammalian Expression, Retroviral |
Yamanaka
| Promotion of direct reprogramming by transformation-deficient Myc.
Proc Natl Acad Sci U S A. 2010 Jul 26. ():.
|
| pcDNA3-FLAG-Lin28A | Lin28A (Homo sapiens) | Mammalian Expression |
Kim
| Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.
Mol Cell. 2008 Oct 24;32(2):276-84. doi: 10.1016/j.molcel.2008.09.014.
|
| pcDNA3-FLAG-Lin28A-mCCHC | Lin28A (Homo sapiens) | Mammalian Expression |
Kim
| Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.
Mol Cell. 2008 Oct 24;32(2):276-84. doi: 10.1016/j.molcel.2008.09.014.
|
| pcDNA3-V5-Lin28A | Lin28A (Homo sapiens) | Mammalian Expression |
Kim
| TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation.
Cell. 2009 Aug 21;138(4):696-708. doi: 10.1016/j.cell.2009.08.002.
|
| FUW-tetO-hOKMS | hOKMS (Homo sapiens) | Mammalian Expression, Lentiviral |
Mikkelsen
| Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency.
Cell. 2015 Jul 16;162(2):412-24. doi: 10.1016/j.cell.2015.06.016.
|
| OCT4-GFP-2A-PURO transgenic reporter | GFP-2A-puro (Synthetic), OCT4 (Homo sapiens) | Mammalian Expression |
Hanna
| Derivation of novel human ground state naive pluripotent stem cells.
Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30.
|
| delta PE OCT4-GFP-2A-PURO transgenic reporter | GFP-2A-puro (Synthetic), OCT4 (Homo sapiens) | Mammalian Expression |
Hanna
| Derivation of novel human ground state naive pluripotent stem cells.
Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30.
|
| delta DE OCT4-GFP-2A-PURO transgenic reporter | GFP-2A-puro (Synthetic), OCT4 (Homo sapiens) | Mammalian Expression |
Hanna
| Derivation of novel human ground state naive pluripotent stem cells.
Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30.
|
| mouse deltaPE Oct4-GFP transgenic reporter | GFP pA (Synthetic), Oct4 (Mus musculus), PGK-gb2-Neo (Synthetic) | Mammalian Expression |
Hanna
| Derivation of novel human ground state naive pluripotent stem cells.
Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30.
|
| pGL3-human OCT4 DE-SV40-Luc | Distal element of human OCT4 promoter (Homo sapiens) | Mammalian Expression, Luciferase |
Hanna
| Derivation of novel human ground state naive pluripotent stem cells.
Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30.
|
| pBABEpuro-CMYC T58A | CMYC (Mus musculus) | Mammalian Expression, Retroviral |
Counter
| Copper is required for oncogenic BRAF signalling and tumorigenesis.
Nature. 2014 Apr 9. doi: 10.1038/nature13180.
|
| pMaster1 | neo (Other), tk (Other), oct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2, myc (Homo sapiens), EBNA-1, Amp (Other), Lin28 (Homo sapiens), Nanog (Homo sapiens), IRES | |
Capecchi
| Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells.
Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7.
|
| pMaster3 | neo (Other), tk (Other), oct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2, myc (Homo sapiens), EBNA-1, Amp (Other), Lin28 (Homo sapiens), Nanog (Homo sapiens), IRES, NR5A2 (Homo sapiens) | |
Capecchi
| Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells.
Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7.
|
| pMaster12 | neo (Other), tk (Other), oct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2 (Homo sapiens), Myc (Homo sapiens), EBNA-1, Amp (Other), Lin28 (Homo sapiens), NANOG (Homo sapiens), IRES, NR5A2 (Homo sapiens), miR302/367 (Homo sapiens) | Mammalian Expression |
Capecchi
| Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells.
Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7.
|
| T7-VEE-OKS-iM | Oct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2 (Homo sapiens), cMyc (Homo sapiens) | Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon |
Dowdy
| Efficient generation of human iPSCs by a synthetic self-replicative RNA.
Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001.
|
| SP6-VEE-OKS-iM | Oct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2 (Homo sapiens), cMyc (Homo sapiens) | Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon |
Dowdy
| Efficient generation of human iPSCs by a synthetic self-replicative RNA.
Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001.
|
| T7-VEE-OKS-iG | Oct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2 (Homo sapiens), GLIS (Homo sapiens) | Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon |
Dowdy
| Efficient generation of human iPSCs by a synthetic self-replicative RNA.
Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001.
|
| SP6-VEE-OKS-iG | Oct4 (Homo sapiens), Klf4 (Homo sapiens), Sox2 (Homo sapiens), GLIS (Homo sapiens) | Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon |
Dowdy
| Efficient generation of human iPSCs by a synthetic self-replicative RNA.
Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001.
|
| pMXs-hGLIS1GR | GLIS1 (Homo sapiens) | Mammalian Expression, Retroviral |
Yamanaka
| Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm.
Nat Commun. 2014 Apr 24;5:3678. doi: 10.1038/ncomms4678.
|
| Nanog-2A-GFP | Nanog (Mus musculus) | Mouse Targeting |
Jaenisch
| Single-cell analysis reveals that expression of nanog is biallelic and equally variable as that of other pluripotency factors in mouse ESCs.
Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 10.1016/j.stem.2013.04.019.
|
| Nanog-2A-mCherry | Nanog (Mus musculus) | Mouse Targeting |
Jaenisch
| Single-cell analysis reveals that expression of nanog is biallelic and equally variable as that of other pluripotency factors in mouse ESCs.
Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 10.1016/j.stem.2013.04.019.
|
| Mouse Nanog locus 3' probe | Nanog (Mus musculus) | Unspecified ; cloning |
Hanna
| Transient acquisition of pluripotency during somatic cell transdifferentiation with iPSC reprogramming factors.
Nat Biotechnol. 2015 Jun 22. doi: 10.1038/nbt.3270.
|
| FUW-TetO-Lin28 | Lin28 (Mus musculus) | Mammalian Expression, Lentiviral |
Jaenisch
| Single-Cell Expression Analyses during Cellular Reprogramming Reveal an Early Stochastic and a Late Hierarchic Phase.
Cell. 2012 Sep 14;150(6):1209-22. doi: 10.1016/j.cell.2012.08.023.
|
| pPB-hCMV1-hNANOG-IV | NANOG (Homo sapiens) | Mammalian Expression |
Smith
| Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human.
Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
|
| pPB-PGK-hKLF4 | KLF4 (Homo sapiens) | Mammalian Expression |
Smith
| Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human.
Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
|
| PpyCAG-NANOG-IB | NANOG (Homo sapiens) | Mammalian Expression |
Smith
| Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human.
Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
|
| pPyCAG-Nanog(T100A)-IP | Mouse Nanog T100A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(F102A)-IP | Mouse Nanog F102A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(Y119A)-IP | Mouse Nanog Y119A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(L122A)-IP | Mouse Nanog L122A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(Q124A)-IP | Mouse Nanog Q124A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(M125A)-IP | Mouse Nanog M125A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(Y136A)-IP | Mouse Nanog Y136A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(K137A)-IP | Mouse Nanog K137A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(Q138A)-IP | Mouse Nanog Q138A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(K140A)-IP | Mouse Nanog K140A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(T141A)-IP | Mouse Nanog T141A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(Q144A)-IP | Mouse Nanog Q144A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(N145A)-IP | Mouse Nanog N145A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(R147A)-IP | Mouse Nanog R147A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pPyCAG-Nanog(K151A)-IP | Mouse Nanog K151A Mutant (Mus musculus) | Mammalian Expression |
Yamanaka
|
Plasmids of NANOG HD Mutants
(unpublished)
|
| pP2A_NLS_sfGFP | Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm (Mus musculus) | Just a donor DNA with Nanog homologous arm for knock-in sfGFP |
Qi
| Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells.
Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 10.1016/j.stem.2015.01.003.
|
| MIP 247 CoMiP 4in1 with shRNA p53 | Mini-intronic-plasmid intron (Homo sapiens) | Mammalian Expression |
Kay
| Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency.
Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
|
| MIP 227 CoMiP 4in1 wo shRNA p53 | Mini-intronic-plasmid intron (Homo sapiens) | Mammalian Expression |
Kay
| Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency.
Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
|
| MIP 339 CoMiP coLin28 2A Nanog IRES eGFP | Lin28 (Homo sapiens), Nanog (Homo sapiens) | Mammalian Expression |
Kay
| Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency.
Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
|
| Luc-Nanog-3'UTR | Nanog homeobox (Mus musculus) | Mammalian Expression |
He
| miR-34 miRNAs provide a barrier for somatic cell reprogramming.
Nat Cell Biol. 2011 Oct 23;13(11):1353-60. doi: 10.1038/ncb2366.
|
| Luc-Nanog-3'UTRmut | Nanog homeobox (Mus musculus) | Mammalian Expression |
He
| miR-34 miRNAs provide a barrier for somatic cell reprogramming.
Nat Cell Biol. 2011 Oct 23;13(11):1353-60. doi: 10.1038/ncb2366.
|
| MOS | OCT4-2A-SOX2 (Homo sapiens) | Mammalian Expression |
Cheng
| A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach.
Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214.
|
| MMK | MYC-2A-KLF4 (Homo sapiens) | Mammalian Expression |
Cheng
| A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach.
Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214.
|
| sgRNA1_NANOG | sgRNA1_NANOG (Homo sapiens) | Mammalian Expression, Lentiviral, CRISPR |
Sato
| CRISPR-Cas9-based Photoactivatable Transcription System.
Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
|
| sgRNA2_NANOG | sgRNA2_NANOG (Homo sapiens) | Mammalian Expression, Lentiviral, CRISPR |
Sato
| CRISPR-Cas9-based Photoactivatable Transcription System.
Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
|
| sgRNA3_NANOG | sgRNA3_NANOG (Homo sapiens) | Mammalian Expression, Lentiviral, CRISPR |
Sato
| CRISPR-Cas9-based Photoactivatable Transcription System.
Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
|
| sgRNA4_NANOG | sgRNA4_NANOG (Homo sapiens) | Mammalian Expression, Lentiviral, CRISPR |
Sato
| CRISPR-Cas9-based Photoactivatable Transcription System.
Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
|
| pRZ-mNanog-YNL | murine Nanog promoter and YNL (Mus musculus) | Lentiviral, Luciferase |
Okada
| Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging.
Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 10.1073/pnas.1418468112. Epub 2015 Mar 23.
|
| pRZ-mOct4-CNL | murine Oct4 promoter and CNL (Mus musculus) | Lentiviral, Luciferase |
Okada
| Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging.
Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 10.1073/pnas.1418468112. Epub 2015 Mar 23.
|
| pGF-mSox2-ONL | murine Sox2 promoter and ONL (Mus musculus) | Lentiviral, Luciferase |
Okada
| Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging.
Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 10.1073/pnas.1418468112. Epub 2015 Mar 23.
|
| pCX-Klf4 | Klf4 (Mus musculus) | Mammalian Expression |
Cohen
|
Pluripotent TFs CRE-seq
(unpublished)
|
| Oct4-Cr1 | gRNA for Crispr targeting the OCT4 stop codon (Homo sapiens) | Mammalian Expression |
Huangfu
| A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells.
Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 10.1016/j.stemcr.2015.04.016.
|
| 6xO/S luc | minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer (Mus musculus) | Mammalian Expression, Luciferase |
Dailey
|
luciferase reporter plasmid containing 6 tandem copies of the composite Oct4/Sox2 binding site of the FGF4 enhancer. Used for detection of active Oct4/Sox2 complexes
(unpublished)
|
| GG-EBNA-OCT4 | 5 concatenated gRNA transcriptional cassettes targeting OCT4 | CRISPR |
Otonkoski
| Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation.
Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001.
|
| pPB-CAG-Klf4-pA-pgk-hph | Klf4 (Mus musculus) | Mammalian Expression |
Silva
| MBD3/NuRD facilitates induction of pluripotency in a context-dependent manner.
Cell Stem Cell. 2014 Jul 3;15(1):102-10. doi: 10.1016/j.stem.2014.04.019. Epub 2014 May 15.
|
| pPB-CAG-cNanog-pgk-hph | Nanog (Gallus gallus) | Mammalian Expression |
Silva
| Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain.
Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
|
| pPB-CAG-mNanogHD-pgk-hph | Nanog (Mus musculus) | Mammalian Expression |
Silva
| Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain.
Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
|
| pPB-CAG-zNanogHD-pgk-hph | Nanog (Danio rerio) | Mammalian Expression |
Silva
| Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain.
Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
|
| pPB-CAG-Flag3-pG-mNanog-pgk-hph | Nanog (Mus musculus) | Mammalian Expression |
Silva
| Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain.
Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
|
| pPB-CAG-Flag3-pG-cNanog-pgk-hph | Nanog (Gallus gallus) | Mammalian Expression |
Silva
| Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain.
Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
|
| pPB-CAG-mNanog-mut-pgk-hph | Nanog (Mus musculus) | Mammalian Expression |
Silva
| Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain.
Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
|
| pPB-CAG-Nanog-pA-pgk-hph | Nanog (Mus musculus) | Mammalian Expression |
Silva
| Nanog overcomes reprogramming barriers and induces pluripotency in minimal conditions.
Curr Biol. 2011 Jan 11;21(1):65-71. doi: 10.1016/j.cub.2010.11.074. Epub 2010 Dec 30.
|
| pPB-CAG-rNanog-pgk-hph | Nanog (Rattus norvegicus) | Mammalian Expression |
Silva
| Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain.
Development. 2011 Nov;138(22):4853-65. doi: 10.1242/dev.068775.
|
| pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo | Klf2 (Mus musculus), Nanog (Mus musculus) | Mammalian Expression |
Silva
| MBD3/NuRD facilitates induction of pluripotency in a context-dependent manner.
Cell Stem Cell. 2014 Jul 3;15(1):102-10. doi: 10.1016/j.stem.2014.04.019. Epub 2014 May 15.
|
| PCXLE-hUL + mTAGBFP2 | LMyc/Lin28 (Homo sapiens) | Mammalian Expression |
Zovein
| Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming.
Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 10.1186/s13287-017-0581-7.
|
| pCXLE-hS + eGFP | Sox2 (Homo sapiens) | Mammalian Expression |
Zovein
| Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming.
Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 10.1186/s13287-017-0581-7.
|
| pCXLE-hK + E2-Crimson | Klf4 (Homo sapiens) | Mammalian Expression |
Zovein
| Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming.
Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 10.1186/s13287-017-0581-7.
|
| pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro | Oct3/4 (Homo sapiens) | Mammalian Expression |
Zovein
| Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming.
Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 10.1186/s13287-017-0581-7.
|
| pCAG-SpCas9-GFP-U6-gRNA | SpCas9 | Mammalian Expression, CRISPR |
Zou
|
pCAG-SpCas9-U6-gRNA
(unpublished)
|
| FUGW-d2GFP-200 | d2GFP + miR-200 family targets | Lentiviral |
Rosen
| The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states.
BMC Biol. 2016 Jun 17;14(1):47. doi: 10.1186/s12915-016-0269-y.
|
| PB-TA-ERN | | Mammalian Expression ; piggyBac transposon |
Woltjen
| Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.
Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
|
| PB-TAC-ERN | | Mammalian Expression ; piggyBac transposon |
Woltjen
| Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.
Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
|
| PB-TAG-ERN | | Mammalian Expression ; piggyBac transposon |
Woltjen
| Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.
Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
|
| PB-TA-ERP2 | | Mammalian Expression ; piggyBac transposon |
Woltjen
| Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.
Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
|
| PB-TAC-ERP2 | | Mammalian Expression ; piggyBac transposon |
Woltjen
| Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.
Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
|
| PB-TAG-ERP2 | | Mammalian Expression ; piggyBac transposon |
Woltjen
| Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.
Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
|
| PB-TAC-OKMS | OKMS (Mus musculus) | Mammalian Expression ; piggyBac transposon |
Woltjen
| KLF4 N-terminal variance modulates induced reprogramming to pluripotency.
Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
|
| PB-TAC-OSKM | OSKM (Mus musculus) | Mammalian Expression ; piggyBac transposon |
Woltjen
| KLF4 N-terminal variance modulates induced reprogramming to pluripotency.
Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
|
| PB-TAC-OK+9MS | OK+9MS (Mus musculus) | Mammalian Expression ; piggyBac transposon |
Woltjen
| KLF4 N-terminal variance modulates induced reprogramming to pluripotency.
Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
|
| PB-TAC-MKOS | MKOS (Mus musculus) | Mammalian Expression ; piggyBac transposon |
Woltjen
| KLF4 N-terminal variance modulates induced reprogramming to pluripotency.
Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
|
| PB-TAC-EB-C5 | lin-28 homolog A (C. elegans) (Mus musculus) | Mammalian Expression ; piggyBac transposon |
Woltjen
| KLF4 N-terminal variance modulates induced reprogramming to pluripotency.
Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
|
| PB-TAC-WTSI | WTSI (Mus musculus) | Mammalian Expression ; piggyBac transposon |
Woltjen
| KLF4 N-terminal variance modulates induced reprogramming to pluripotency.
Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
|
| pDONR223_NANOG_WT | NANOG (Homo sapiens) | Gateway Entry vector |
Boehm
| Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles.
Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
|
| pDONR223_SOX2_WT | SOX2 (Homo sapiens) | Gateway Entry vector |
Boehm
| Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles.
Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
|
| pREPROII-attB | Oct4 (Mus musculus), cMyc (Mus musculus), Sox2 (Mus musculus), Kruppel-like factor 4 (Mus musculus) | Mammalian Expression, Cre/Lox |
Kuehn
| Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector.
PLoS One. 2013;8(1):e55170. doi: 10.1371/journal.pone.0055170. Epub 2013 Jan 31.
|
| pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro | SOX2-T2A-2xNLS-TdTomato-F2A-Puro (Homo sapiens) | CRISPR |
Otonkoski
| Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9.
Stem Cell Res. 2017 Jul;22:16-19. doi: 10.1016/j.scr.2017.05.005. Epub 2017 May 17.
|
| pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro | OCT4-T2A-NLS-EmGFP-P2A-Puro (Homo sapiens) | CRISPR |
Otonkoski
| Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9.
Stem Cell Res. 2017 Aug;23:105-108. doi: 10.1016/j.scr.2017.07.006. Epub 2017 Jul 11.
|
| pLenti_CamKIIa_GFP | Green fluorescent protein | Lentiviral |
Dolmetsch
| SHANK3 and IGF1 restore synaptic deficits in neurons from 22q13 deletion syndrome patients.
Nature. 2013 Nov 14;503(7475):267-71. doi: 10.1038/nature12618. Epub 2013 Oct 16.
|
| pLenti_CamKIIa_mKate2 | far-red fluorescent protein | Lentiviral |
Dolmetsch
| SHANK3 and IGF1 restore synaptic deficits in neurons from 22q13 deletion syndrome patients.
Nature. 2013 Nov 14;503(7475):267-71. doi: 10.1038/nature12618. Epub 2013 Oct 16.
|
| pSimple-miR302/367 | miR302/367 (miRNA cluster) (Homo sapiens) | Mammalian Expression |
Thomson
| Simultaneous Reprogramming and Gene Correction of Patient Fibroblasts.
Stem Cell Reports. 2015 Dec 8;5(6):1109-18. doi: 10.1016/j.stemcr.2015.10.009. Epub 2015 Nov 12.
|
| pSN16 | vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN17 | vexGFP-P2A-OCT4 fused to full length HP1 alpha (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN20 | vexGFP-P2A-OCT4 fused to a 'medium' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN21 | vexGFP-P2A-OCT4 fused to a 'weak' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN22 | mCitrine-P2A-SOX2 fused to a 'strong' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN23 | mCitrine-P2A-SOX2 fused to a 'medium' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN24 | mCitrine-P2A-SOX2 fused to a 'weak' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN25 | mCitrine-P2A-SOX2 fused to full length HP1 alpha (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN26 | mCerulean-P2A-cMYC fused to a 'strong' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN27 | mCerulean-P2A-cMYC fused to a 'medium' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN28 | mCerulean-P2A-cMYC fused to a 'weak' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN29 | mCerulean-P2A-cMYC fused to full length HP1 alpha (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN30 | mCherry-P2A-KLF4 fused to a 'strong' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN31 | mCherry-P2A-KLF4 fused to a 'medium' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN32 | mCherry-P2A-KLF4 fused to a 'weak' VP16 domain (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN33 | mCherry-P2A-KLF4 fused to full length HP1 alpha (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN34 | vexGFP-P2A-OCT4 fused to 2 VP16 domains (413-460) separated by linkers (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN35 | vexGFP-P2A-OCT4 fused to an Engrailed domain (168-281) separated by a linker (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN36 | vexGFP-P2A-OCT4 fused to 1 VP16 domain (413-460) separated by a linker (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN61 | vexGFP-P2A-putative OCT4 DBD (138-289) with a T7 tag (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN65 | mCitrine-P2A-putative SOX2 DBD (41-109) with a V5 tag (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN67 | mCherry-P2A-putative KLF4 DBD with a 6xHis tag (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| pSN69 | mCerulean-P2A-putative cMYC DBD with a HA tag (Homo sapiens) | Mammalian Expression, Lentiviral |
Ptashne
| OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071.
|
| U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE | Ascl1, Brn2 (Mus musculus) | Lentiviral |
Parmar
| REST suppression mediates neural conversion of adult human fibroblasts via microRNA-dependent and -independent pathways.
EMBO Mol Med. 2017 Aug;9(8):1117-1131. doi: 10.15252/emmm.201607471.
|
| PB-GG-OCT4-1-5-PGK-Puro | OCT4-gRNAs-1-5-PGK-puro (Other) | Mammalian Expression, CRISPR |
Otonkoski
| Human pluripotent reprogramming with CRISPR activators.
Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
|
| pUCM-AAVS1-TO-hNGN2 | Neurogenin 2 (Homo sapiens), mCherry (Synthetic), rtTA3G (Other) | CRISPR, TALEN |
Ward
| Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons.
Curr Protoc Cell Biol. 2018 Jun;79(1):e51. doi: 10.1002/cpcb.51. Epub 2018 May 18.
|
| pUCM-CLYBL-hNIL | Neurogenin 2 (Homo sapiens), ISL LIM Homeobox 1 (Homo sapiens), LIM Homeobox 3 (Homo sapiens), mCherry (Synthetic), rtTA3G (Other) | CRISPR, TALEN |
Ward
| Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons.
Curr Protoc Cell Biol. 2018 Jun;79(1):e51. doi: 10.1002/cpcb.51. Epub 2018 May 18.
|
| pX-APRT-sg2 | APRT sgRNA-2 | Mammalian Expression, CRISPR |
Woltjen
| Microhomology-assisted scarless genome editing in human iPSCs.
Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
|
| p3-HPRT1-S104R-PdTK-mCh | HPRT genomic DNA (Homo sapiens), puro-deltaTK; CAG::mCherry | |
Woltjen
| Microhomology-assisted scarless genome editing in human iPSCs.
Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
|
| p3-HPRT1-S104Rf-PdTK-mCh | HPRT genomic DNA (Homo sapiens), puro-deltaTK; CAG::mCherry | |
Woltjen
| Microhomology-assisted scarless genome editing in human iPSCs.
Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
|
| pbG-APRT-J-u32uni-PdTKmCh | APRT (Homo sapiens), puro-deltaTK; CAG::mCherry | |
Woltjen
| Microhomology-assisted scarless genome editing in human iPSCs.
Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
|
| pAAVS1-PdTK-CAG-mCh-[uBglII] | AAVS1 puro-deltaTK; CAG-mCherry | |
Woltjen
| Microhomology-assisted scarless genome editing in human iPSCs.
Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
|
| pCAG-eGFP-pA | GFP | Mammalian Expression |
Woltjen
| Microhomology-assisted scarless genome editing in human iPSCs.
Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
|
| H52-pUCM-hNGN2-inv | Neurogenin 2 (Homo sapiens), mcherry (Other), rtTA3G | Mammalian Expression, Cre/Lox, CRISPR, TALEN |
Ward
|
A comprehensive guide for transcription-factor mediated differentiation of human iPSC into neurons
(unpublished)
|
| pMx-puro-MGT | Mef2c, Tbx5 and Gata4 (Mus musculus) | Mammalian Expression, Retroviral |
Qian
| Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency and quality of induced cardiac myocyte reprogramming.
Circ Res. 2015 Jan 16;116(2):237-44. doi: 10.1161/CIRCRESAHA.116.305547. Epub 2014 Nov 21.
|
| pMx-MGT | Mef2c, Tbx5 and Gata4 (Mus musculus) | Mammalian Expression, Retroviral |
Qian
| Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency and quality of induced cardiac myocyte reprogramming.
Circ Res. 2015 Jan 16;116(2):237-44. doi: 10.1161/CIRCRESAHA.116.305547. Epub 2014 Nov 21.
|
| pAAVS1-TRE-MA4-GFP | MLL-AF4 fusion gene (Homo sapiens) | Mammalian Expression |
Kan
| Respecifying human iPSC-derived blood cells into highly engraftable hematopoietic stem and progenitor cells with a single factor.
Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2180-2185. doi: 10.1073/pnas.1718446115. Epub 2018 Jan 31.
|
| pLV-PGK-SNAP-MD-SOX2 | SNAP-MD-SOX2 (Synthetic) | Lentiviral |
Suter
| A role for mitotic bookmarking of SOX2 in pluripotency and differentiation.
Genes Dev. 2016 Nov 15;30(22):2538-2550. doi: 10.1101/gad.289256.116. Epub 2016 Dec 5.
|
| pLV-PGK-SNAP-MDMUT-SOX2 | SNAP-MDMUT-SOX2 (Synthetic) | Lentiviral |
Suter
| A role for mitotic bookmarking of SOX2 in pluripotency and differentiation.
Genes Dev. 2016 Nov 15;30(22):2538-2550. doi: 10.1101/gad.289256.116. Epub 2016 Dec 5.
|
| pKIOct4-Halo-IRES-Bsd | OCT4-Halo-IRES-Bsd (Synthetic) | Mammalian Expression |
Suter
| Endogenous fluctuations of OCT4 and SOX2 bias pluripotent cell fate decisions
bioRxiv 299073
|
| tetO.Sox9.Puro | Sox9 (Mus musculus) | Mammalian Expression, Lentiviral |
Ahlenius
| Rapid and efficient induction of functional astrocytes from human pluripotent stem cells.
Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2. Epub 2018 Aug 20.
|
| tetO.Nfia.Blast | Nfia (Mus musculus) | Mammalian Expression, Lentiviral |
Ahlenius
| Rapid and efficient induction of functional astrocytes from human pluripotent stem cells.
Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2. Epub 2018 Aug 20.
|
| tetO.Nfib.Hygro | Nfib (Mus musculus) | Mammalian Expression, Lentiviral |
Ahlenius
| Rapid and efficient induction of functional astrocytes from human pluripotent stem cells.
Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2. Epub 2018 Aug 20.
|
| pMX-eSOX2NRR | eSOX2NRR (Mus musculus) | Mammalian Expression, Retroviral |
Jauch
| Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing.
Stem Cell Reports. 2018 Aug 14;11(2):593-606. doi: 10.1016/j.stemcr.2018.07.002. Epub 2018 Aug 2.
|
| pMX-eSOX2AHK | eSox2AHK (Mus musculus) | Mammalian Expression, Retroviral |
Jauch
| Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing.
Stem Cell Reports. 2018 Aug 14;11(2):593-606. doi: 10.1016/j.stemcr.2018.07.002. Epub 2018 Aug 2.
|
| pMX-eSOX17FNV | eSOX17FNV (Mus musculus) | Mammalian Expression, Retroviral |
Jauch
| Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing.
Stem Cell Reports. 2018 Aug 14;11(2):593-606. doi: 10.1016/j.stemcr.2018.07.002. Epub 2018 Aug 2.
|
| pMX-eSOX17WHC | eSOX17WHC (Mus musculus) | Mammalian Expression, Retroviral |
Jauch
| Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing.
Stem Cell Reports. 2018 Aug 14;11(2):593-606. doi: 10.1016/j.stemcr.2018.07.002. Epub 2018 Aug 2.
|
| EF1a_KLF4_P2A_Hygro_Barcode | KLF4 (Homo sapiens) | Lentiviral |
Mali
| Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout.
Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008.
|
| EF1a_SOX2_P2A_Hygro_Barcode | SOX2 (Homo sapiens) | Lentiviral |
Mali
| Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout.
Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008.
|
| CLYBL-TO-hNGN2-BSD-mApple | NGN2 (Homo sapiens) | Mammalian Expression |
Ward
|
Next generation vectors for transcription factor mediated differentiation of iPSCs into cortical and motor neurons
(unpublished)
|
| CLYBL-TO-hNIL-BSD-mApple | NGN2, ISL1, LHX3 (Homo sapiens) | Mammalian Expression |
Ward
|
Next generation vectors for transcription factor mediated differentiation of iPSCs into cortical and motor neurons
(unpublished)
|
| AICSDP-60: SOX2-mEGFP | SOX2 Homology Arms with linker-mEGFP (Homo sapiens) | Mammalian Expression, CRISPR ; Donor Template |
Allen Institute for Cell Science
|
Allen Institute for Cell Science Plasmids
(unpublished)
|
| Sox2-t2A-GFP | SOX2 (Homo sapiens) | Mammalian Expression |
Mitchell
| KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control.
Genes Dev. 2019 Jun 20. pii: gad.324319.119. doi: 10.1101/gad.324319.119.
|
| Sox2-t2A-mCherry | SOX2 (Homo sapiens) | Mammalian Expression |
Mitchell
| KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control.
Genes Dev. 2019 Jun 20. pii: gad.324319.119. doi: 10.1101/gad.324319.119.
|
| Nanog-t2A-mCherry | Nanog (Mus musculus) | Mammalian Expression |
Mitchell
| KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control.
Genes Dev. 2019 Jun 20. pii: gad.324319.119. doi: 10.1101/gad.324319.119.
|
| FUW-TetO-Gata3 | Gata3 (Mus musculus) | Lentiviral |
Buganim
| Extensive Nuclear Reprogramming Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells.
Cell Stem Cell. 2015 Nov 5;17(5):543-56. doi: 10.1016/j.stem.2015.08.006. Epub 2015 Sep 24.
|
| FUW-TetO-Tfap2c | Tfap2c (Mus musculus) | Lentiviral |
Buganim
| Extensive Nuclear Reprogramming Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells.
Cell Stem Cell. 2015 Nov 5;17(5):543-56. doi: 10.1016/j.stem.2015.08.006. Epub 2015 Sep 24.
|
| FUW-TetO-Gata6 | Gata6 (Mus musculus) | Lentiviral |
Buganim
| Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.
Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
|
| FUW-TetO-Sox17 | Sox17 (Mus musculus) | Lentiviral |
Buganim
| Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.
Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
|
| FUW-TetO-Cdx2 | Cdx2 (Mus musculus) | Lentiviral |
Buganim
| Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.
Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
|
| FUW-TetO-Klf5 | Klf5 (Mus musculus) | Lentiviral |
Buganim
| Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.
Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
|
| FUW-TetO-Sox15 | Sox15 (Mus musculus) | Lentiviral |
Buganim
| Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.
Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
|
| FUW-TetO-Tead4 | Tead4 (Mus musculus) | Lentiviral |
Buganim
| Extensive Nuclear Reprogramming Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells.
Cell Stem Cell. 2015 Nov 5;17(5):543-56. doi: 10.1016/j.stem.2015.08.006. Epub 2015 Sep 24.
|
| pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS | Nuclear EYFP into Elf5 locus (Mus musculus) | Mouse Targeting |
Buganim
| Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.
Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
|
| pNTKV- Utf1-KI-3'UTR-2A-tdTomato-RBGpA | tdTomato into Utf1 locus (Mus musculus) | Mouse Targeting |
Buganim
| Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.
Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
|
| pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA | tagBFP into Esrrb locus (Mus musculus) | Mouse Targeting |
Buganim
| Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.
Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
|
| pLVH-EF1a-GFP-P2A-OCT4 | human OCT4 (Homo sapiens) | Lentiviral |
Hu
| The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis.
Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869.
|
| pLVH-EF1a-GFP-P2A-SOX2 | human SOX2 (Homo sapiens) | Lentiviral |
Hu
| The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis.
Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869.
|
| pLVH-EF1a-GFP-P2A-KLF4 | human KLF4 (Homo sapiens) | Lentiviral |
Hu
| The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis.
Nat Commun. 2016 Mar 7;7:10869. doi: 10.1038/ncomms10869.
|
| pCW57-GFP-miR-302cluster | human miR-302 cluster: MIR302B, MIR302C, MIR302A, MIR302D, MIR367 (Homo sapiens) | Mammalian Expression, Lentiviral ; Doxycycline inducible, microRNA expression |
Bárta
| Oct4-mediated reprogramming induces embryonic-like microRNA expression signatures in human fibroblasts.
Sci Rep. 2019 Oct 31;9(1):15759. doi: 10.1038/s41598-019-52294-3.
|
| Sox2-SE-RGM-eGFP targeting vector | Sox2-SE-5arm-RGM-Sox2-SE-3arm (Mus musculus) | Mouse Targeting |
Jaenisch
| Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs.
Mol Cell. 2019 Aug 15. pii: S1097-2765(19)30502-7. doi: 10.1016/j.molcel.2019.06.045.
|