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Wolfe-Lawson ZFN modular assembly kit
(Kit # 1000000018 )

Depositing Labs:   Scot Wolfe, Nathan Lawson

This plasmid kit contains reagents for engineering zinc finger arrays by modular assembly method.

This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$350 USD + shipping

Available to academics and nonprofits only.

Original Publication

Evaluation and application of modularly assembled zinc finger nucleases in zebrafish. Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ, Look AT, Epstein JA, Lawson ND, and Wolfe SA. Development 2011, 138:4555-4564. PubMed

Description

Zinc-finger nucleases (ZFNs) allow targeted gene inactivation in a wide range of model organisms. We provided an archive of 81 clones containing 27 different zinc-finger modules at finger 1, 2 and 3 positions for straightforward construction of ZFNs using modular assembly-based approach. The majority of zinc-finger proteins assembled from these modules have favorable specificities and nearly one-third of modular ZFNs generated lesions at their targets in the zebra fish genome. These modules are designed for the construction of three-finger zinc-finger ZFNs, but they can also be used for assembling longer ZFNs containing 4 or more modules.

Figure : Modules containing in this archive can recognize the following triplet target sites (5’→3’):

Wolfe-Lawson ZFN kit image.jpg

Kit Documentation

Icon Wolfe-Lawson ZFN kit (286.1 KB)

Resources

We set up a website to help users search appropriate sites in their target sequences and design corresponding ZFNs using this archive:

http://pgfe.umassmed.edu/ZFPmodularsearch.html

To facilitate the application of ZFNs within the zebrafish community we constructed a public database of sites in the zebrafish genome that can be targeted using this archive:

http://pgfe.umassmed.edu/ZFNV1/

Protocols

Protocols for modular assembly construction of ZFNs can be found at the above website as well as in the publication.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The plasmid kit used for generation of ZFNs was a gift from Scot Wolfe and Nathan Lawson (Addgene kit # 1000000018)"

For your Reference section:
Evaluation and application of modularly assembled zinc finger nucleases in zebrafish. Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ, Look AT, Epstein JA, Lawson ND, and Wolfe SA. Development 2011, 138:4555-4564. PubMed

Wolfe-Lawson ZFN modular assembly kit - #1000000018

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin

Inventory

Well Plasmid Resistance
A / 1 pBS-F1-AAG
Ampicillin
A / 2 pBS-F1-ACG
Ampicillin
A / 3 pBS-F1-AGG
Ampicillin
A / 4 pBS-F1-ATG
Ampicillin
A / 5 pCS2-F1-CAG-F2-GAC
Ampicillin
A / 6 pBS-F1-CGG
Ampicillin
A / 7 pBS-F1-GAA
Ampicillin
A / 8 pCS2_F1-GAC
Ampicillin
A / 9 pCS2-F1-GAG-F2-GAG-F3-AAG
Ampicillin
A / 10 pCS2-F2-GAA
Ampicillin
A / 11 pCS2-F1-GCA
Ampicillin
A / 12 pCS2-F1-GCC
Ampicillin
B / 1 pCS2-F1-GCG
Ampicillin
B / 2 pCS2-F1-GCT
Ampicillin
B / 3 pCS2-F1-GGA-F2-GTG-F3-TTG
Ampicillin
B / 4 pCS2-F1-GGC
Ampicillin
B / 5 pBS-F1-GGG
Ampicillin
B / 6 pCS2-F1-GGT-F3-CAG
Ampicillin
B / 7 pBS-F1-GTA
Ampicillin
B / 8 pBS-F1-GTC
Ampicillin
B / 9 pCS2-F1-GTG-F2-GGT-F3-GAA
Ampicillin
B / 10 pCS2_F1-GTT
Ampicillin
B / 11 pBS-F1-TAG
Ampicillin
B / 12 pCS2-F1-TGG-F3-GGG
Ampicillin
C / 1 pBS-F1-TGT
Ampicillin
C / 2 pBS-F1-TTG
Ampicillin
C / 3 pBS-F2-AAG
Ampicillin
C / 4 pCS2-F2-ACG
Ampicillin
C / 5 pBS-F2-AGG
Ampicillin
C / 6 pBS-F2-ATG
Ampicillin
C / 7 pBS-F2-CAG
Ampicillin
C / 8 pBS-F2-CGG
Ampicillin
C / 9 pBS-F2-GAT
Ampicillin
C / 10 pCS2-F2-GCA
Ampicillin
C / 11 pBS-F2-GCC
Ampicillin
C / 12 pCS2-F2-GCG
Ampicillin
D / 1 pCS2-F2-GCT
Ampicillin
D / 2 pBS-F2-GGA
Ampicillin
D / 3 pCS2-F2-GGC
Ampicillin
D / 4 pBS-F3-GAG
Ampicillin
D / 5 pBS-F2-GTA
Ampicillin
D / 6 pBS-F2-GTC
Ampicillin
D / 7 pCS2-F2-GTT-F3-GGC
Ampicillin
D / 8 pBS-F2-TAG
Ampicillin
D / 9 pCS2-F2-TGG
Ampicillin
D / 10 pBS-F2-TGT
Ampicillin
D / 11 pBS-F2-TTG
Ampicillin
D / 12 pBS-F3-ACG
Ampicillin
E / 1 pBS-F3-AGG
Ampicillin
E / 2 pBS-F3-ATG
Ampicillin
E / 3 pBS-F3-CGG
Ampicillin
E / 4 pBS-F3-GAC
Ampicillin
E / 5 pCS2-F3-GAT
Ampicillin
E / 6 pBS-F3-GCC
Ampicillin
E / 7 pCS2-F3-GCT
Ampicillin
E / 8 pBS-F3-GGA
Ampicillin
E / 9 pCS2-F3-GGT
Ampicillin
E / 10 pBS-F3-GTA
Ampicillin
E / 11 pBS-F3-GTC
Ampicillin
E / 12 pCS2-F3-GTG
Ampicillin
F / 1 pBS-F3-GTT
Ampicillin
F / 2 pBS-F3-TAG
Ampicillin
F / 3 pBS-F3-TGG
Ampicillin
F / 4 pCS2-F3-TGT
Ampicillin
F / 5 pBS-F3-GCG
Ampicillin
F / 6 pCS2-F2-GGG
Ampicillin
F / 7 pBS-F1-GAT
Ampicillin
F / 8 pCS2-F3-GCA
Ampicillin
F / 9 pCS2-F1-AGA
Ampicillin
F / 10 pBS-F2-AGA
Ampicillin
F / 11 pBS-F3-AGA
Ampicillin
H / 1 pCS2-HA-Kdrl-3p-FokI-KK
Ampicillin
H / 2 pCS2-Flag-Kdrl-5p-FokI-EL
Ampicillin
Data calculated @ 2016-12-08

Kit Plate Map - #1000000018

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