1,779 results

1. pGLOW30

   Plasmid

   #172697

   Purpose

   C. elegans mScarlet co-injection marker

   Depositor

   Ryan Doonan

   Article

   Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

   Insert

   Pmyo-2::mScarlet
   

   Tags

   mScarlet

   Expression

   Worm

   Promoter

   myo-2

   Available Since

   Sept. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pDD268

   Plasmid

   #132523

   Purpose

   mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Daniel Dickinson, Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mNG-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized mNG

   Expression

   Worm

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHW522 (Prab-3::cGAL-C::let-858 3'UTR)

   Plasmid

   #107130

   Purpose

   cGAL-C driver under the control of C. elegans rab-3 promoter

   Depositor

   Paul Sternberg

   Article

   Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

   Insert

   NLS::gp41-1-C-intein::cGAL(AD)
   

   Expression

   Worm

   Promoter

   C. elegans rab-3 promoter

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)

   Plasmid

   #107133

   Purpose

   cGAL-N driver under the control of C. elegans hsp-16.41 promoter

   Depositor

   Paul Sternberg

   Article

   Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

   Insert

   NLS::cGAL(DBD)::gp41-1-N-intein
   

   Expression

   Worm

   Promoter

   C. elegans hsp-16.41 promoter

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

   Plasmid

   #107137

   Purpose

   Dominant negative PKA cGAL effector for C. elegans

   Depositor

   Paul Sternberg

   Article

   Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

   Insert

   kin-2a(G310D)::SL2::GFP
   

   Expression

   Worm

   Promoter

   15xUAS-delta-pes-10(basal promoter)

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPD95_77

   Plasmid

   #1495

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Tags

   IVSA, rf2204, gfpN, IVSR, gfpS65C, IVSS, IVST, gf…

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ90 - Pmyo-2::mCherry::unc-54utr

   Plasmid

   #19327

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Pmyo-2::mCherry
   

   Expression

   Worm

   Available Since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIP_RNA1

   Plasmid

   #61259

   Purpose

   Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

   Depositor

   David Wang

   Article

   Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

   Insert

   Orsay RNA1
   

   Expression

   Worm

   Promoter

   heat shock promoter

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCZGY3196

   Plasmid

   #172696

   Purpose

   Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)

   Depositor

   Yishi Jin

   Article

   Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

   Insert

   Prgef-1
   

   Tags

   GFP 1-10

   Expression

   Worm

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. DR274

    Plasmid

    #42250

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR; Zebrafish expression

    Expression

    Worm

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIP_RNA2

    Plasmid

    #61260

    Purpose

    Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA2
    

    Expression

    Worm

    Promoter

    heat shock promoter

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDJ39 - Peft-3 Cre (PATCs)

    Plasmid

    #191381

    Purpose

    High efficiency CRE recombinase expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Peft-3 Cre tbb-2 UTR
    

    Expression

    Worm

    Promoter

    Peft-3

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ104 - Pmyo-3::mCherry::unc-54

    Plasmid

    #19328

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-3::mCherry
    

    Tags

    mCherry

    Expression

    Worm

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid

    #154840

    Purpose

    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Mutation

    HYGR∆ encodes aa 1-226

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMS79 (eft-3p::Cas9 + sgRNA)

    Plasmid

    #154839

    Purpose

    Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    sgRNA
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS114

    Plasmid

    #215677

    Purpose

    Empty repair plasmid for ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxN + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJL43.1 - Pglh-2::MosTase::glh-2utr

    Plasmid

    #19332

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pglh-2::Mos1 transposase
    

    Expression

    Worm

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMS59

    Plasmid

    #215680

    Purpose

    Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMS158

    Plasmid

    #215679

    Purpose

    Empty repair plasmid for ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMS77

    Plasmid

    #215681

    Purpose

    Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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