1,767 results

1. pGLOW30

   Plasmid

   #172697

   Purpose

   C. elegans mScarlet co-injection marker

   Depositor

   Ryan Doonan

   Article

   Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

   Insert

   Pmyo-2::mScarlet
   

   Use

   Tags

   mScarlet

   Expression

   Worm

   Mutation

   Promoter

   myo-2

   Available since

   Sept. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. DR274

   Plasmid

   #42250

   Purpose

   guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR; Zebrafish expression

   Tags

   Expression

   Worm

   Mutation

   Promoter

   T7

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

   Plasmid

   #159798

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJL43.1 - Pglh-2::MosTase::glh-2utr

   Plasmid

   #19332

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Pglh-2::Mos1 transposase
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCFJ90 - Pmyo-2::mCherry::unc-54utr

   Plasmid

   #19327

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Pmyo-2::mCherry
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEM318 - sgRNA mosTI unc-119 single-copy

   Plasmid

   #159822

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI unc-119 single-copy
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 3xHA-TurboID_pAS31

   Plasmid

   #118220

   Purpose

   expresses 3xHA-tagged TurboID in C. elegans intestine

   Depositor

   Jessica Feldman

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   3x HA tag

   Expression

   Worm

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   ges-1p

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGM34DEST

   Plasmid

   #74756

   Purpose

   Encodes for the 5X repeat of the QUAS response element, followed by the delta pes-10 minimal promoter

   Depositor

   Keith Yamamoto, Jordan Ward

   Article

   Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

   Insert

   Gateway cassette
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJL046

   Plasmid

   #198807

   Purpose

   In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::HisCl1-SL2-GFP::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEM319 - array co-injection fragment mosTI unc-119

    Plasmid

    #159823

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI unc-119
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

    Plasmid

    #154824

    Purpose

    (eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    Peft-3::wrmScarlet::tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

    Plasmid

    #24334

    Depositor

    Didier Stainier

    Article

    Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

    Insert

    H2B
    

    Use

    Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Mutation

    mCherry driven by an HSP70 promoterCerulean drive…

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWXC078

    Plasmid

    #222961

    Purpose

    C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

    Depositor

    Oliver Hobert

    Article

    Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

    Insert

    mScarlet-I3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. L2531

    Plasmid

    #1607

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    myo2-1090, myo25', IVSA, rf2128, IVSM, IVSL,…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP::L4440

    Plasmid

    #11335

    Depositor

    Guy Caldwell

    Insert

    GFP
    

    Use

    RNAi

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pdestMB14_sur5p-dCas9-VP64

    Plasmid

    #177788

    Purpose

    Overexpression of dCas9-VP64 in C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    dCas9-VP64
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCE-BiFC-VC155

    Plasmid

    #22020

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Nat Protoc. 2008 . 3(4):588-96.

    Insert

    VC155
    

    Use

    Tags

    HA

    Expression

    Worm

    Mutation

    Changed Alanine 206 to Lysine

    Promoter

    Available since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCE-BiFC-VN173

    Plasmid

    #22019

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Nat Protoc. 2008 . 3(4):588-96.

    Insert

    VN173
    

    Use

    Tags

    Myc

    Expression

    Worm

    Mutation

    N/A

    Promoter

    Available since

    Oct. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEM246 - MCS mosTI unc-119 target

    Plasmid

    #159821

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI unc-119 target
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge