1,786 results

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pSEM238

Plasmid

#161515

Purpose

Neuronal expression of histamine-sensitive chloride channel for negative selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Psnt-1 | histamine | rpl-3 UTR]

Expression

Worm

Promoter

Psnt-1

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pWXC078

Plasmid

#222961

Purpose

C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

Depositor

Oliver Hobert

Article

Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

Insert

mScarlet-I3

Expression

Worm

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMDJ231 - Phsp Cas9

Plasmid

#191382

Purpose

Heat shock inducible Cas9 expression in C. elegans.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Phsp-16.41 Cas9 gpd-2 tagRFP-t

Use

CRISPR

Expression

Worm

Promoter

Phsp-16.41

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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L4440gtwy

Plasmid

#11344

Depositor

Guy Caldwell

Insert

gateway cassette

Use

RNAi

Expression

Worm

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra2

Plasmid

#184933

Purpose

Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2

Expression

Worm

Promoter

tag-168

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM272 - MCS mosTI Pmlc-2_GFP target

Plasmid

#159834

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI Pmlc-2_GFP target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHygRR2r

Plasmid

#200076

Purpose

hygR selection mScarlet 2X marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM255 - sgRNA mosTI Pmlc-2_GFP

Plasmid

#159835

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI Pmlc-2_GFP

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBN282

Plasmid

#86727

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pelt-2::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMNK56 - piRNAi 21ur-1224 - him-8

Plasmid

#159819

Purpose

piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

Depositor

Christian Froekjaer-Jensen

Article

Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

Insert

him-8 piRNAi 21ur-1224

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

Plasmid

#182352

Purpose

Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

Plasmid

#154824

Purpose

(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

Peft-3::wrmScarlet::tbb-2 UTR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSM.wrmScarlet1-10::SL2::GFP1-10

Plasmid

#234318

Purpose

For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans

Depositor

Kota Mizumoto

Article

Kurashina et al Genetics. 2024 Dec 22:iyae214. doi: 10.1

Insert

wrmScarlet1-10::SL2::GFP1-10

Expression

Worm

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

Plasmid

#159899

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | tagRFP | cbr-tbb-2 UTR

Expression

Worm

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

Plasmid

#85583

Purpose

cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

Prab-3-GAL4-SK(DBD)-VP64

Expression

Worm

Promoter

Prab-3-GAL4-SK(DBD)-VP64

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pID2.02

Plasmid

#26955

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’

Expression

Worm

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pGC93

Plasmid

#19628

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pSEM231 - [Pmlc-1 | gfp | cbr-tbb-2 UTR]

Plasmid

#159897

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | gfp | cbr-tbb-2 UTR

Expression

Worm

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGC162

Plasmid

#19633

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP680-T2A-HO1

Plasmid

#197244

Purpose

Expresses the protein of wmiRFP680-T2A-HO1 in neurons of C elegan

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP680-T2A-HO1

Expression

Worm

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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