1,779 results

1. pSKI

   Plasmid

   #232484

   Purpose

   pSKI can be used for single-copy transgene expression within the SKI PLACE System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

   Depositor

   Carlos Silva García

   Article

   Dinneen et al G3 (Bethesda). 2025 Sep 19:jkaf220. doi:

   Insert

   pSKI
   

   Expression

   Worm

   Available Since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGK-EGFP-FKBPF36V

   Plasmid

   #199765

   Purpose

   plasmid for targeting dTAG into the AAVS1 locus

   Depositor

   Hannes Lans

   Article

   van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

   Insert

   GFP-dTAG
   

   Tags

   GFP and dTAG

   Expression

   Mammalian and Worm

   Promoter

   hPGK

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

   Plasmid

   #154824

   Purpose

   (eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

   Depositor

   Patrick Phillips

   Article

   Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

   Insert

   Peft-3::wrmScarlet::tbb-2 UTR
   

   Expression

   Worm

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF11-wElectra2

   Plasmid

   #184933

   Purpose

   Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   Electra2
   

   Expression

   Worm

   Promoter

   tag-168

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGLOW30

   Plasmid

   #172697

   Purpose

   C. elegans mScarlet co-injection marker

   Depositor

   Ryan Doonan

   Article

   Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

   Insert

   Pmyo-2::mScarlet
   

   Tags

   mScarlet

   Expression

   Worm

   Promoter

   myo-2

   Available Since

   Sept. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDD268

   Plasmid

   #132523

   Purpose

   mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Daniel Dickinson, Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mNG-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized mNG

   Expression

   Worm

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHW522 (Prab-3::cGAL-C::let-858 3'UTR)

   Plasmid

   #107130

   Purpose

   cGAL-C driver under the control of C. elegans rab-3 promoter

   Depositor

   Paul Sternberg

   Article

   Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

   Insert

   NLS::gp41-1-C-intein::cGAL(AD)
   

   Expression

   Worm

   Promoter

   C. elegans rab-3 promoter

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. DR274

   Plasmid

   #42250

   Purpose

   guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR; Zebrafish expression

   Expression

   Worm

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFJ90 - Pmyo-2::mCherry::unc-54utr

   Plasmid

   #19327

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Pmyo-2::mCherry
   

   Expression

   Worm

   Available Since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)

    Plasmid

    #107133

    Purpose

    cGAL-N driver under the control of C. elegans hsp-16.41 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Expression

    Worm

    Promoter

    C. elegans hsp-16.41 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPD95_77

    Plasmid

    #1495

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    IVSA, rf2204, gfpN, IVSR, gfpS65C, IVSS, IVST, gf…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

    Plasmid

    #107137

    Purpose

    Dominant negative PKA cGAL effector for C. elegans

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    kin-2a(G310D)::SL2::GFP
    

    Expression

    Worm

    Promoter

    15xUAS-delta-pes-10(basal promoter)

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCZGY3196

    Plasmid

    #172696

    Purpose

    Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    Prgef-1
    

    Tags

    GFP 1-10

    Expression

    Worm

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHIP_RNA1

    Plasmid

    #61259

    Purpose

    Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA1
    

    Expression

    Worm

    Promoter

    heat shock promoter

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMDJ39 - Peft-3 Cre (PATCs)

    Plasmid

    #191381

    Purpose

    High efficiency CRE recombinase expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Peft-3 Cre tbb-2 UTR
    

    Expression

    Worm

    Promoter

    Peft-3

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHIP_RNA2

    Plasmid

    #61260

    Purpose

    Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA2
    

    Expression

    Worm

    Promoter

    heat shock promoter

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFJ104 - Pmyo-3::mCherry::unc-54

    Plasmid

    #19328

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-3::mCherry
    

    Tags

    mCherry

    Expression

    Worm

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid

    #154840

    Purpose

    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Mutation

    HYGR∆ encodes aa 1-226

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJL43.1 - Pglh-2::MosTase::glh-2utr

    Plasmid

    #19332

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pglh-2::Mos1 transposase
    

    Expression

    Worm

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMS79 (eft-3p::Cas9 + sgRNA)

    Plasmid

    #154839

    Purpose

    Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    sgRNA
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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