1,774 results

1. pSM-mStayGold

   Plasmid

   #234317

   Purpose

   codon-optimized mStayGold with synthetic introns for the expression in C. elegans

   Depositor

   Kota Mizumoto

   Article

   Ko et al MicroPubl Biol. 2025 Jan 18;2025:10.1791

   Insert

   C. elegans codon optimized mStayGOld
   

   Use

   Tags

   mStayGold

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCZGY3196

   Plasmid

   #172696

   Purpose

   Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)

   Depositor

   Yishi Jin

   Article

   Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

   Insert

   Prgef-1
   

   Use

   Tags

   GFP 1-10

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pflp-18::NGL-1::spGFP11

   Plasmid

   #65828

   Purpose

   For trans-synaptic labeling

   Depositor

   Kang Shen, Cori Bargmann

   Article

   Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

   Insert

   NLG-1 (nlg-1 Nematode)
   

   Use

   Tags

   spGFP11

   Expression

   Worm

   Mutation

   N142S

   Promoter

   Pflp-18

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS1-Prig-3::tagBFP

   Plasmid

   #165598

   Purpose

   rig-3 promoter derive BFP expression in AVA head neurons.

   Depositor

   Andrew Leifer

   Article

   Hallinen et al Elife. 2021 Jul 29;10:e66135. doi: 10.75

   Insert

   Prig-3, tagBFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

   Plasmid

   #159849

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBN41

   Plasmid

   #86716

   Purpose

   C. elegans injection marker

   Depositor

   Peter Askjaer

   Article

   Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

   Insert

   Pmyo-2::GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xHA-TurboID_pAS31

   Plasmid

   #118220

   Purpose

   expresses 3xHA-tagged TurboID in C. elegans intestine

   Depositor

   Jessica Feldman

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   3x HA tag

   Expression

   Worm

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   ges-1p

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEY121

   Plasmid

   #223468

   Purpose

   sra-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   sra-6 (sra-6 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSKI

   Plasmid

   #232484

   Purpose

   pSKI can be used for single-copy transgene expression within the SKI PLACE System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

   Depositor

   Carlos Silva García

   Article

   Dinneen et al G3 (Bethesda). 2025 Sep 19:jkaf220. doi:

   Insert

   pSKI
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDYH5

    Plasmid

    #52032

    Purpose

    Expression of GFP-tagged DAF-16 in C. elegans

    Depositor

    Meng-Qiu Dong

    Article

    Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

    Insert

    DAF-16 (daf-16 Nematode)
    

    Use

    Tags

    GFP and HIS

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pEY11

    Plasmid

    #191017

    Purpose

    Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    Ultrapanneuronal
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid

    #47549

    Purpose

    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Inserts

    Cas9
    Empty sgRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    U6 and eef-1A.1 (eft-3)

    Available Since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. L4440

    Plasmid

    #1654

    Depositor

    Andrew Fire

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi

    Tags

    T7p, T7p, lacZN, OriF1>>, OriF1<<

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJL046

    Plasmid

    #198807

    Purpose

    In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::HisCl1-SL2-GFP::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWXC077

    Plasmid

    #222960

    Purpose

    C. elegans codon-optimized mScarlet3 with 3 introns cloned into pPD95.75 backbone

    Depositor

    Oliver Hobert

    Article

    Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

    Insert

    mScarlet3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMLS485

    Plasmid

    #188890

    Purpose

    Worm Cas9 expression vector, Peft-3 promoter

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Peft-3::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDO068

    Plasmid

    #198788

    Purpose

    Drives specific expression in the BAG neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Pgcy-33-GAL4-SK(DBD)-VP64
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWXC078

    Plasmid

    #222961

    Purpose

    C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

    Depositor

    Oliver Hobert

    Article

    Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

    Insert

    mScarlet-I3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

    Plasmid

    #154824

    Purpose

    (eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    Peft-3::wrmScarlet::tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge