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Plasmid#91724Purposemycobacterial expression of mCherry reporterDepositorTypeEmpty backboneUseUnspecified; Mycobacterial expressionTagsmCherryExpressionMutationPromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only
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pKM402
Plasmid#107770PurposeExpression of phage Che9c RecT for oligo-mediated recombineering in mycobacteriaDepositorInsertsChe9C RecT
TetR (tet repressor)
cat
kan
UseTagsExpressionBacterialMutationPromoterCat promoter, Ptet, kan promoter, and pTB21Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLO87
Plasmid#91726Purposemycobacterial expression of mCherry from Hsp60 promoterDepositorInserthsp60 promoter
UseMycobacterial expressionTagsmCherryExpressionMutationPromoterhsp60Available sinceMay 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM444
Plasmid#108319PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage IntegraseDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM461
Plasmid#108320PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacBDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTiGc
Plasmid#78314PurposeMycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).DepositorInsertsTurboFP635
GFP
UseSynthetic Biology; Mycobacterial replicating vect…TagsExpressionBacterialMutationPromoterhsp60 and hsp60(ribo)Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJDC60
Plasmid#157780PurposetdTomato is expressed under the mycobacterial L5 promoter for in vivo and in vitro detection.DepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterL5Available sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMyCA
Plasmid#84268PurposeA versatile vector for recombinant mycobacterial protein productionDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterAceteamidaseAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM512
Plasmid#140192PurposeFor curing of Orbit-integrated plasmids and associated drug markerDepositorInsertszeocin resistance gene
Bxb1gp47 (directionality factor)
UseTagsExpressionBacterialMutationCloned in gp47 directinality factor in place of R…PromoterEM7 and PtetAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKW08-Lx
Plasmid#25012DepositorInsertpKW08-Lx
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKW08-Lx-Int
Plasmid#25015DepositorInsertpKW08-Lx-Int
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMEND-Lx
Plasmid#25010DepositorInsertpMEND-Lx
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMIND-Lx
Plasmid#25013DepositorInsertpMIND-Lx
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMEND-Lx-Int
Plasmid#25014DepositorInsertpMEND-Lx-Int
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDB88
Plasmid#26958DepositorInserte. coli galactokinase
UseTagsExpressionBacterialMutationcloned under the MOP promoterPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNitET-SacB-kan
Plasmid#107692PurposeExpression of the phage Che9c RecET system for Recombineering in mycobacteriaDepositorInsertsChe9 phage RecET
PNit2-NitR
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCre-SacB-zeo
Plasmid#107706PurposeExpresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.DepositorInsertble (Zeo resistance)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM342
Plasmid#71486PurposeContains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.DepositorInsertloxP-hyg-loxP cassette
UseSource of loxp-hyg-loxp for pcr amplification.TagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM343
Plasmid#71487PurposeContains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.DepositorInsertloxP-hyg-cat-loxP
UseSource of loxp-hyg-cat- loxp for pcr amplificatio…TagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMyNT-kan
Plasmid#84693PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterAcetamidaseAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only