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pAAV.CAG.Flex.Twitch2B.WPRE.SV40

Plasmid

#100039

Purpose

AAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch2B

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFX-Pickles 2.34NES

Plasmid

#100028

Purpose

Improved FRET biosensor for the measurement of BCR-ABL activity.

Depositor

Yusuke Ohba

Article

Horiguchi et al Cell Struct Funct. 2017 Feb 2;42(1):15-2

Insert

Pickles-2.34NES

Use

Pfx vector is derived from pcmv vector.

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn1.Twitch2B.WPRE.SV40

Plasmid

#100040

Purpose

AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Has Service

AAV1

Insert

Twitch2B

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet_huCAD_ICADL (TevS)

Plasmid

#100098

Purpose

dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

Depositor

Jim Wells

Article

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

DNA fragmentation factor subunit alpha (CAD, DFFA Human)

Use

Tags

Expression

Bacterial

Mutation

two caspase cleavage sites (DETD-117 and DAVD-224…

Promoter

T7

Available since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ131D2.0

Plasmid

#100044

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

OsU3

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pOD2045-intControl

Plasmid

#100005

Purpose

MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).

Depositor

Karen Oegema

Article

Wang et al Development. 2017 Jun 15. pii: dev.15009

Inserts

zif-1 (zif-1 Nematode)
unc-119

Use

Targeting vector for mos1 transposon mediated sin…

Tags

Expression

Mutation

Promoter

Available since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCHW100002

Plasmid

#222440

Purpose

Level 0, CDS module (AATG-GCTT) containing rice OsL5.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

OsL5 (Oryza sativa)

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BPLF1_1-1000

Plasmid

#198582

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BPLF1_1-1000

Use

Gateway entry vector

Tags

Expression

Mutation

None (wildtype)

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PG13-gal (wt p53 binding sites)

Plasmid

#16446

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
PG13-luc (wt p53 binding sites)

Plasmid

#16442

Purpose

Luciferase reporter containing 13 copies of the p53-binding consensus sequence

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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MG15-luc (mut p53 binding sites)

Plasmid

#16443

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (mutant) (TP53 )

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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MG15-gal (mut p53 binding sites)

Plasmid

#16447

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (mutant) (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
N-Cadherin-EGFP

Plasmid

#18870

Depositor

Valeri Vasioukhin

Article

Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

Insert

N-Cadherin (Cdh2 Mouse)

Use

Tags

EGFP, Kanamycin, and N-Cadherin

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pUAS-Ncad-GFP

Plasmid

#35986

Depositor

Stephen Smith, James Jontes

Article

Jontes et al J Neurosci. 2004 Oct 13;24(41):9027-34.

Insert

Ncad (cdh2 Zebrafish)

Use

Gal4 inducible

Tags

EGFP

Expression

Mutation

Promoter

14xUAS-E1b

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1- Ncad-GFP

Plasmid

#35676

Depositor

James Jontes

Article

Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

Insert

Ncad (cdh2 Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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1099-N-cadherin-AAA-YFP

Plasmid

#32237

Depositor

Kathleen Green

Article

Chen et al J Cell Biol. 2003 Nov 10;163(3):547-57.

Insert

N-cadherin (Cdh2 Mouse)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Glu–Glu–Asp (aa 780–782) ---> Ala–Ala–Ala

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
JT113-pETDuet1-(R)-hREV3L

Plasmid

#64872

Purpose

bacterial expression of human REV3L

Depositor

Richard Wood

Article

Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

Insert

REV3L (REV3L Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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Rab32b cds in pGem-T Easy

Plasmid

#80550

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab32b (rab32b Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Rab8b cds in pGem-T Easy

Plasmid

#80525

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab8b (rab8b Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Rab34b cds in pGem-T Easy

Plasmid

#80554

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab34b (rab34b Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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