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Wolfe-Lawson ZFN modular assembly kit
(Kit # 1000000018 )

Depositing Labs:   Scot Wolfe, Nathan Lawson

This plasmid kit contains reagents for engineering zinc finger arrays by modular assembly method.

This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80oC immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$350 USD + shipping
Available to Academic and Nonprofits Only

Original Publication

Evaluation and application of modularly assembled zinc finger nucleases in zebrafish. Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ, Look AT, Epstein JA, Lawson ND, and Wolfe SA. Development 2011, 138:4555-4564. PubMed

Description

Zinc-finger nucleases (ZFNs) allow targeted gene inactivation in a wide range of model organisms. We provided an archive of 81 clones containing 27 different zinc-finger modules at finger 1, 2 and 3 positions for straightforward construction of ZFNs using modular assembly-based approach. The majority of zinc-finger proteins assembled from these modules have favorable specificities and nearly one-third of modular ZFNs generated lesions at their targets in the zebra fish genome. These modules are designed for the construction of three-finger zinc-finger ZFNs, but they can also be used for assembling longer ZFNs containing 4 or more modules.

Figure: Modules containing in this archive can recognize the following triplet target sites (5’→3’):

Wolfe-Lawson ZFN kit image.jpg

Kit Documentation

Icon Wolfe-Lawson ZFN kit (286.1 KB)

Resources

We set up a website to help users search appropriate sites in their target sequences and design corresponding ZFNs using this archive:

http://pgfe.umassmed.edu/ZFPmodularsearch.html

To facilitate the application of ZFNs within the zebrafish community we constructed a public database of sites in the zebrafish genome that can be targeted using this archive:

http://pgfe.umassmed.edu/ZFNV1/

Protocols

Protocols for modular assembly construction of ZFNs can be found at the above website as well as in the publication.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The plasmid kit used for generation of ZFNs was a gift from Scot Wolfe and Nathan Lawson (Addgene kit # 1000000018)"

For your Reference section:
Evaluation and application of modularly assembled zinc finger nucleases in zebrafish. Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ, Look AT, Epstein JA, Lawson ND, and Wolfe SA. Development 2011, 138:4555-4564. PubMed

Resistance Color Key:

 
  Ampicillin  

Inventory

Well Plasmid Resistance
A / 1 pBS-F1-AAG
  Ampicillin
A / 2 pBS-F1-ACG
  Ampicillin
A / 3 pBS-F1-AGG
  Ampicillin
A / 4 pBS-F1-ATG
  Ampicillin
A / 5 pCS2-F1-CAG-F2-GAC
  Ampicillin
A / 6 pBS-F1-CGG
  Ampicillin
A / 7 pBS-F1-GAA
  Ampicillin
A / 8 pCS2_F1-GAC
  Ampicillin
A / 9 pCS2-F1-GAG-F2-GAG-F3-AAG
  Ampicillin
A / 10 pCS2-F2-GAA
  Ampicillin
A / 11 pCS2-F1-GCA
  Ampicillin
A / 12 pCS2-F1-GCC
  Ampicillin
B / 1 pCS2-F1-GCG
  Ampicillin
B / 2 pCS2-F1-GCT
  Ampicillin
B / 3 pCS2-F1-GGA-F2-GTG-F3-TTG
  Ampicillin
B / 4 pCS2-F1-GGC
  Ampicillin
B / 5 pBS-F1-GGG
  Ampicillin
B / 6 pCS2-F1-GGT-F3-CAG
  Ampicillin
B / 7 pBS-F1-GTA
  Ampicillin
B / 8 pBS-F1-GTC
  Ampicillin
B / 9 pCS2-F1-GTG-F2-GGT-F3-GAA
  Ampicillin
B / 10 pCS2_F1-GTT
  Ampicillin
B / 11 pBS-F1-TAG
  Ampicillin
B / 12 pCS2-F1-TGG-F3-GGG
  Ampicillin
C / 1 pBS-F1-TGT
  Ampicillin
C / 2 pBS-F1-TTG
  Ampicillin
C / 3 pBS-F2-AAG
  Ampicillin
C / 4 pCS2-F2-ACG
  Ampicillin
C / 5 pBS-F2-AGG
  Ampicillin
C / 6 pBS-F2-ATG
  Ampicillin
C / 7 pBS-F2-CAG
  Ampicillin
C / 8 pBS-F2-CGG
  Ampicillin
C / 9 pBS-F2-GAT
  Ampicillin
C / 10 pCS2-F2-GCA
  Ampicillin
C / 11 pBS-F2-GCC
  Ampicillin
C / 12 pCS2-F2-GCG
  Ampicillin
D / 1 pCS2-F2-GCT
  Ampicillin
D / 2 pBS-F2-GGA
  Ampicillin
D / 3 pCS2-F2-GGC
  Ampicillin
D / 4 pBS-F3-GAG
  Ampicillin
D / 5 pBS-F2-GTA
  Ampicillin
D / 6 pBS-F2-GTC
  Ampicillin
D / 7 pCS2-F2-GTT-F3-GGC
  Ampicillin
D / 8 pBS-F2-TAG
  Ampicillin
D / 9 pCS2-F2-TGG
  Ampicillin
D / 10 pBS-F2-TGT
  Ampicillin
D / 11 pBS-F2-TTG
  Ampicillin
D / 12 pBS-F3-ACG
  Ampicillin
E / 1 pBS-F3-AGG
  Ampicillin
E / 2 pBS-F3-ATG
  Ampicillin
E / 3 pBS-F3-CGG
  Ampicillin
E / 4 pBS-F3-GAC
  Ampicillin
E / 5 pCS2-F3-GAT
  Ampicillin
E / 6 pBS-F3-GCC
  Ampicillin
E / 7 pCS2-F3-GCT
  Ampicillin
E / 8 pBS-F3-GGA
  Ampicillin
E / 9 pCS2-F3-GGT
  Ampicillin
E / 10 pBS-F3-GTA
  Ampicillin
E / 11 pBS-F3-GTC
  Ampicillin
E / 12 pCS2-F3-GTG
  Ampicillin
F / 1 pBS-F3-GTT
  Ampicillin
F / 2 pBS-F3-TAG
  Ampicillin
F / 3 pBS-F3-TGG
  Ampicillin
F / 4 pCS2-F3-TGT
  Ampicillin
F / 5 pBS-F3-GCG
  Ampicillin
F / 6 pCS2-F2-GGG
  Ampicillin
F / 7 pBS-F1-GAT
  Ampicillin
F / 8 pCS2-F3-GCA
  Ampicillin
F / 9 pCS2-F1-AGA
  Ampicillin
F / 10 pBS-F2-AGA
  Ampicillin
F / 11 pBS-F3-AGA
  Ampicillin
H / 1 pCS2-HA-Kdrl-3p-FokI-KK
  Ampicillin
H / 2 pCS2-Flag-Kdrl-5p-FokI-EL
  Ampicillin
Data from: CALCULATED @ 2014-12-22 18:25:13.324272