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Addgene

pDC183
(Plasmid #106415)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106415 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pETDuet-1
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SNAP25 fragment 141-204
  • Alt name
    SNAP25
  • Species
    R. norvegicus (rat), Synthetic
  • Mutation
    Fragment 141-204, Codon optimization
  • Entrez Gene
    Snap25 (a.k.a. SNAP-25B, SNAP-25a)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pET Upstream Primer #69214-3
  • 3′ sequencing primer DuetDOWN1 Primer #71179-3
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    SNAP25 Fragment 7-83
  • Alt name
    SNAP25
  • Species
    R. norvegicus (rat), Synthetic
  • Mutation
    Fragment 7-83, Codon optimization
  • GenBank ID
    P60880
  • Entrez Gene
    Snap25 (a.k.a. SNAP-25B, SNAP-25a)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer DuetUP2 Primer #71180-3
  • 3′ sequencing primer T7 Terminator Primer #69337-3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDC183 was a gift from Axel Brunger (Addgene plasmid # 106415 ; http://n2t.net/addgene:106415 ; RRID:Addgene_106415)
  • For your References section:

    The primed SNARE-complexin-synaptotagmin complex for neuronal exocytosis. Zhou Q, Zhou P, Wang AL, Wu D, Zhao M, Sudhof TC, Brunger AT. Nature. 2017 Aug 24;548(7668):420-425. doi: 10.1038/nature23484. Epub 2017 Aug 16. 10.1038/nature23484 PubMed 28813412