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RINS1(mut)
(Plasmid #107291)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107291 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 107291-D50 50 μg of DNA in Tris buffer $495
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DNA 107291-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin, mCherry, sfGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RINS1(mut)
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1812
  • Mutation
    two Arg/Ser mutations at the beginning and in the end of the C-chain of insulin to prevent the cleavege of C-peptide upon insulin maturation
  • Promoter CMV
  • Tag / Fusion Protein
    • sfGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F: CGCAAATGGGCGGTAGGCGTG; pEGFP-FP:TTTAGTGAACCGTCAGATC
  • 3′ sequencing primer pEGFP_C2-RP: TTTAAAGCAAGTAAAACCTC; SV40pA-R: GAAATTTGTGATGCTATTGC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RINS1(mut) was a gift from Dmytro Yushchenko (Addgene plasmid # 107291 ; http://n2t.net/addgene:107291 ; RRID:Addgene_107291)

  • For your References section:

    A Ratiometric Sensor for Imaging Insulin Secretion in Single beta Cells. Schifferer M, Yushchenko DA, Stein F, Bolbat A, Schultz C. Cell Chem Biol. 2017 Apr 20;24(4):525-531.e4. doi: 10.1016/j.chembiol.2017.03.001. Epub 2017 Mar 30. 10.1016/j.chembiol.2017.03.001 PubMed 28366620
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