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pcDNA6 MCV LTco S239A
(Plasmid #112194)


Item Catalog # Description Quantity Price (USD)
Plasmid 112194 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4985
  • Total vector size (bp) 7549
  • Modifications to backbone
    pcDNA6.V5.HisB vector was modified with NheI and PmeI restriction digestion enzymes to cut out MCS, V5 and His tag sites. New multiple cloning sites using annealed primer pair were ligated back into the vector. The newly generated cloning site comprises: NheI, KpnI, EcoRV, XhoI and SacII. Codon optimized Large T was cloned between EcoRV and XhoI restriction sites.
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    MCPyV LT codon optimized
  • Alt name
    MCV LT
  • Alt name
    MCV large T antigen
  • Alt name
    Merkel Cell Polyomavirus large T antigen
  • Species
    Merkel cell polyomavirus
  • Insert Size (bp)
  • GenBank ID
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHr
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA6 MCV LTco S239A was a gift from Patrick Moore (Addgene plasmid # 112194 ; ; RRID:Addgene_112194)
  • For your References section:

    Protein-mediated viral latency is a novel mechanism for Merkel cell polyomavirus persistence. Kwun HJ, Chang Y, Moore PS. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4040-E4047. doi: 10.1073/pnas.1703879114. Epub 2017 May 1. 10.1073/pnas.1703879114 PubMed 28461484