|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11336||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerL4440 is available at Addgene
- Backbone size w/o insert (bp) 2790
Vector typeWorm Expression, RNAi
Growth in Bacteria
Copy numberHigh Copy
SpeciesC. elegans (nematode)
Insert Size (bp)1200
- Cloning method Restriction Enzyme
- 5′ sequencing primer L4440 primer (AGCGAGTCAGTGAGCGAG) (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byDr. Andrew Fire created the vector backbone L4440
Terms and Licenses
- Not Available to Industry
Full length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:lis-1::L4440 was a gift from Guy Caldwell (Addgene plasmid # 11336 ; http://n2t.net/addgene:11336 ; RRID:Addgene_11336)