pAAV-hSyn-sbGLuc-CheRiff-EGFP
(Plasmid #114108)

• Purpose: fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics
• Depositing Lab: Ute Hochgeschwender
• Publication: luciferase opsin fusion proteins for bioluminescent optogenetics (unpublished)

Backbone

• Vector backbone: pAAV
  (Search Vector Database)
• Backbone size w/o insert (bp): 4269
• Total vector size (bp): 6611
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Gaussia luciferase, slow burn
• Alt name: sbGLuc

Gene/Insert 2

• Gene/Insert name: CheRiff, synthetic ChR
• Alt name: CheRiff

Gene/Insert 3

• Gene/Insert name: Enhanced Green Fluorescent Protein
• Alt name: EGFP

Resource Information

• Supplemental Documents:
  • pAAV hSyn sbGluc CheRiff EGFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry