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pCR2.1 GFR alpha 2
(Plasmid #119899)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119899 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GDNF family receptor alpha-2
  • Alt name
    Gfra2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Gfra2

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

This plasmid can be used to generate an in situ hybridization antisense probe for mouse Gfr-alpha2. Acc65I or SpeI can be used to linearize the plasmid followed by SP6 to transcribe the ~1.5kb probe.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR2.1 GFR alpha 2 was a gift from David Ginty & Wenqin Luo (Addgene plasmid # 119899 ; http://n2t.net/addgene:119899 ; RRID:Addgene_119899)
  • For your References section:

    Molecular identification of rapidly adapting mechanoreceptors and their developmental dependence on ret signaling. Luo W, Enomoto H, Rice FL, Milbrandt J, Ginty DD. Neuron. 2009 Dec 24;64(6):841-56. doi: 10.1016/j.neuron.2009.11.003. 10.1016/j.neuron.2009.11.003 PubMed 20064391