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UbcH5c WT pET28a Citations (13)

Originally described in: A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination.
Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE Mol Cell. 2006 Mar 17. 21(6):873-80.
PubMed Journal

Articles Citing UbcH5c WT pET28a

Ubiquitination directly enhances activity of the deubiquitinating enzyme ataxin-3. Todi SV, Winborn BJ, Scaglione KM, Blount JR, Travis SM, Paulson HL. EMBO J. 2009 Feb 18. 28(4):372-82. PubMed
The RING heterodimer BRCA1-BARD1 is a ubiquitin ligase inactivated by the platinum-based anticancer drugs. Atipairin A, Canyuk B, Ratanaphan A. Breast Cancer Res Treat. 2011 Feb;126(1):203-9. doi: 10.1007/s10549-010-1182-7. Epub 2010 Sep 29. PubMed
Activity and cellular functions of the deubiquitinating enzyme and polyglutamine disease protein ataxin-3 are regulated by ubiquitination at lysine 117. Todi SV, Scaglione KM, Blount JR, Basrur V, Conlon KP, Pastore A, Elenitoba-Johnson K, Paulson HL. J Biol Chem. 2010 Dec 10. 285(50):39303-13. PubMed
Substitution of aspartic acid with glutamic acid at position 67 of the BRCA1 RING domain retains ubiquitin ligase activity and zinc(II) binding with a reduced transition temperature. Atipairin A, Canyuk B, Ratanaphan A. J Biol Inorg Chem. 2011 Feb;16(2):217-26. doi: 10.1007/s00775-010-0718-y. Epub 2010 Oct 22. PubMed
Arabidopsis membrane-anchored ubiquitin-fold (MUB) proteins localize a specific subset of ubiquitin-conjugating (E2) enzymes to the plasma membrane. Dowil RT, Lu X, Saracco SA, Vierstra RD, Downes BP. J Biol Chem. 2011 Apr 29;286(17):14913-21. doi: 10.1074/jbc.M110.158808. Epub 2011 Feb 23. PubMed
p300 Acetyltransferase activity differentially regulates the localization and activity of the FOXO homologues in skeletal muscle. Senf SM, Sandesara PB, Reed SA, Judge AR. Am J Physiol Cell Physiol. 2011 Jun;300(6):C1490-501. doi: 10.1152/ajpcell.00255.2010. Epub 2011 Mar 9. PubMed
High-Yield Expression in Escherichia coli and Purification of Mouse Ubiquitin-Activating Enzyme E1. Carvalho AF, Pinto MP, Grou CP, Vitorino R, Domingues P, Yamao F, Sa-Miranda C, Azevedo JE. Mol Biotechnol. 2011 Oct 20. PubMed

Associated Plasmids

In Vitro Enhanced Sensitivity to Cisplatin in D67Y BRCA1 RING Domain Protein. Atipairin A, Ratanaphan A. Breast Cancer (Auckl). 2011;5:201-8. doi: 10.4137/BCBCR.S8184. Epub 2011 Sep 25. PubMed
Auto-ubiquitination of Mdm2 enhances its substrate ubiquitin ligase activity. Ranaweera RS, Yang X. J Biol Chem. 2013 Jun 28;288(26):18939-46. doi: 10.1074/jbc.M113.454470. Epub 2013 May 13. PubMed
Most mutations that cause spinocerebellar ataxia autosomal recessive type 16 (SCAR16) destabilize the protein quality-control E3 ligase CHIP. Kanack AJ, Newsom OJ, Scaglione KM. J Biol Chem. 2018 Feb 23;293(8):2735-2743. doi: 10.1074/jbc.RA117.000477. Epub 2018 Jan 9. PubMed
UbcH5 Interacts with Substrates to Participate in Lysine Selection with the E3 Ubiquitin Ligase CHIP. Kanack A, Vittal V, Haver H, Keppel T, Gundry RL, Klevit RE, Scaglione KM. Biochemistry. 2020 Jun 9;59(22):2078-2088. doi: 10.1021/acs.biochem.0c00084. Epub 2020 May 27. PubMed
Polyamines Counteract Carbonate-Driven Proteasome Stalling in Alkaline Conditions. Kudriaeva AA, Saratov GA, Kaminskaya AN, Vladimirov VI, Barzilovich PY, Belogurov AA Jr. Biomolecules. 2020 Nov 24;10(12). pii: biom10121597. doi: 10.3390/biom10121597. PubMed
The molecular basis of spinocerebellar ataxia type 48 caused by a de novo mutation in the ubiquitin ligase CHIP. Umano A, Fang K, Qu Z, Scaglione JB, Altinok S, Treadway CJ, Wick ET, Paulakonis E, Karunanayake C, Chou S, Bardakjian TM, Gonzalez-Alegre P, Page RC, Schisler JC, Brown NG, Yan D, Scaglione KM. J Biol Chem. 2022 May;298(5):101899. doi: 10.1016/j.jbc.2022.101899. Epub 2022 Apr 7. PubMed

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