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pTRE3G-Bi-β-chain
(Plasmid #133731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133731 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 133731-D50 50 μg of DNA in Tris buffer $495
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DNA 133731-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTRE3G-BI-ZsGreen1
  • Backbone manufacturer
    TakaraBio Clontech
  • Backbone size w/o insert (bp) 2839
  • Total vector size (bp) 3847
  • Modifications to backbone
    The backbone for this plasmid is ‘pTRE3G-BI-ZsGreen1’ from the pTRE3G-BI-ZsGreen1 Vector Set (Cat. No. 631334) from TakaraBio Clontech. We inserted the gePSI-bChain downstream of the pTRE3G promoter using Gibson Cloning.
  • Vector type
    Mammalian Expression
  • Selectable markers
    No selectable marker

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    b-Chain_gePSI
  • Species
    Zea mays
  • Insert Size (bp)
    312
  • Mutation
    Only one part of the gePSI-system (startcodon + aa167-269)
  • Promoter pTRE3G-Bi

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    ZsGreen1
  • Species
    Zoanthus sp.
  • Insert Size (bp)
    696
  • Promoter pTRE3G-Bi

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE3G-Bi-β-chain was a gift from Erin Schuman (Addgene plasmid # 133731 ; http://n2t.net/addgene:133731 ; RRID:Addgene_133731)

  • For your References section:

    A genetically encodable cell-type-specific protein synthesis inhibitor. Heumuller M, Glock C, Rangaraju V, Biever A, Schuman EM. Nat Methods. 2019 Jul 15. pii: 10.1038/s41592-019-0468-x. doi: 10.1038/s41592-019-0468-x. 10.1038/s41592-019-0468-x PubMed 31308551
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