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pBS L30 Ruby3-Rab7A
(Plasmid #135651)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 135651 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS
  • Modifications to backbone
    Modified for mammalian expression using the promoter of the mouse ribosomal protein L30 gene ("weak promoter") and a HSV TK polyA tail
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab7A
  • Alt name
    Rab7
  • Alt name
    ras-related protein Rab-7a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    624
  • GenBank ID
    NP_004628.4 7879
  • Entrez Gene
    RAB7A (a.k.a. CMT2B, PRO2706, RAB7)
  • Promoter L30
  • Tag / Fusion Protein
    • Ruby3 (N terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS L30 Ruby3-Rab7A was a gift from Raphael Gaudin (Addgene plasmid # 135651 ; http://n2t.net/addgene:135651 ; RRID:Addgene_135651)
  • For your References section:

    CRISPR-based bioengineering of the Transferrin Receptor revealed a role for Rab7 in the biosynthetic secretory pathway. Maika S. Deffieu, Ieva Cesonyte, François Delalande, Gaelle Boncompain, Cristina Dorobantu, Eli Song, Vincent Lucansky, Aurélie Hirschler, Sarah Cianferani, Tao Xu, Franck Perez, Christine Carapito, Raphael Gaudin. Science Advances 10.1126/sciadv.aba7803