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pCEP4-PDGFRA-reporter
(Plasmid #136456)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 136456 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10375
  • Total vector size (bp) 15506
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    PDGFRalpha
  • Alt name
    PDGFRA
  • Alt name
    platelet-derived growth factor receptor alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3324
  • GenBank ID
    NP_006197.1
  • Entrez Gene
    PDGFRA (a.k.a. CD140A, PDGFR-2, PDGFR2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Signal peptide from influenza HA (N terminal on insert)
    • Extracellular c-myc epitope tag

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-fwd
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
  • Species
    Synthetic
  • Insert Size (bp)
    1807
  • Promoter Minimal promoter with artificial SRE enhancer.
  • Tag / Fusion Protein
    • The PEST sequence from mouse ornithine decarboxylase (MODC) is fused to GFP to reduce its stability. (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The minimal promoter and SRE enhancer were subcloned from Addgene plasmid 82686 (SRE reporter vector_559)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-PDGFRA-reporter was a gift from Erik Procko (Addgene plasmid # 136456 ; http://n2t.net/addgene:136456 ; RRID:Addgene_136456)
  • For your References section:

    Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology. Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, Procko E. PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 10.1371/journal.ppat.1008647. eCollection 2020 Jun. 10.1371/journal.ppat.1008647 PubMed 32559251