Purpose(Empty Backbone) Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||137993||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer TTTCCCATGATTCCTTCATATTTGC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://docs.brocklab.com/clonmapper for an updated protocol.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Crop-Seq-BFP-WPRE-TS-hU6-BsmbI was a gift from Amy Brock (Addgene plasmid # 137993 ; http://n2t.net/addgene:137993 ; RRID:Addgene_137993)
For your References section:Functionalized Lineage Tracing for the Study and Manipulation of Heterogeneous Cell Populations. Gardner A, Morgan D, Al'Khafaji A, Brock A. Methods Mol Biol. 2022;2394:109-131. doi: 10.1007/978-1-0716-1811-0_8. 10.1007/978-1-0716-1811-0_8 PubMed 35094325