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(Plasmid #13887)


Item Catalog # Description Quantity Price (USD)
Plasmid 13887 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 7500
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    C domain of N-WASP
  • Alt name
  • Alt name
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
  • Mutation
    The BglII site in the MCS of pShuttle-CMV was filled and the vector was religated in order to remove this BglII site.
  • GenBank ID
  • Entrez Gene
    WASL (a.k.a. BOS_4408)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site HinDIII (not destroyed)
  • 5′ sequencing primer GGCACCAAAATCAACGGGACTTTCC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original full length N-WASP gene clone was given to me by Dr. Laura Machesky School of Biosciences The University of Birmingham Edgbaston Birmingham B15 2TT UK [email protected]

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pShuttle-CMV-EGFP-C was a gift from Lorene Lanier (Addgene plasmid # 13887 ; ; RRID:Addgene_13887)
  • For your References section:

    Arp2/3 is a negative regulator of growth cone translocation. Strasser GA, Rahim NA, VanderWaal KE, Gertler FB, Lanier LM. Neuron. 2004 Jul 8. 43(1):81-94. 10.1016/j.neuron.2004.05.015 PubMed 15233919