pDONR221-SARS-CoV-2 S1 RBD
(Plasmid
#167013)
-
PurposeGateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2
-
Depositing Lab
-
Depositing OrganizationCentre for Research and Technology Hellas (CERTH)
Located in Greece -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167013 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 167013-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 167013-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
* Log in to view industry pricing.
Backbone
-
Vector backbonepDONR221
- Backbone size w/o insert (bp) 4762
- Total vector size (bp) 5434
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameS
-
SpeciesSARS-CoV-2 isolate Wuhan-Hu-1
-
Insert Size (bp)672
-
MutationNo stop codon
-
GenBank ID
-
Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byCloned from pDONR223 SARS-CoV-2 S (Addgene #149329) received from Addgene
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 167013-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 167013-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDONR221-SARS-CoV-2 S1 RBD was a gift from Spyros Petrakis (Addgene plasmid # 167013 ; http://n2t.net/addgene:167013 ; RRID:Addgene_167013) -
For your References section:
AI Promoted Virtual Screening, Structure-Based Hit Optimization, and Synthesis of Novel COVID-19 S-RBD Domain Inhibitors. Gkekas I, Katsamakas S, Mylonas S, Fotopoulou T, Magoulas GEpsilon, Tenchiu AC, Dimitriou M, Axenopoulos A, Rossopoulou N, Kostova S, Wanker EE, Katsila T, Papahatjis D, Gorgoulis VG, Koufaki M, Karakasiliotis I, Calogeropoulou T, Daras P, Petrakis S. J Chem Inf Model. 2024 Nov 13. doi: 10.1021/acs.jcim.4c01110. 10.1021/acs.jcim.4c01110 PubMed 39535926
