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XE127 Rfz1HA-CS2+
(Plasmid #16797)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 16797 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Entrez Gene
  • Tag / Fusion Protein
    • HA (internal)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by: Julia Yang-Snyder (subclone), Jeff Brown (tags), 4/19/96.

Comments: Rfz1 from Henk Roelink. This sequence has a few differences.
from the published sequence. Sites listed in Rfz1 are not a complete list.
and I haven't searched through the CS2+ sequence to see if/where those sites.
are present. HA tag version made by Jeff Brown (2/98-3/98) insert the epitope.
into the StyI site (1904). The 3' Sty site is destroyed by the tags, but.
the 5' site is inteact. Epitope can be detected in PBS/Formaldehyde fixed.
tissue permeablized with 0.2% Triton. Frizzled tends to ppt out of SDS sample.
buffer when boiled, so either warm samples to ~70 degrees Celsius for a.
few minutes before loading samples or use a strong denaturing loading buffer.
and incubate the samples at room temp for half and hour before loading.
The StyI site is in the 3rd extracellular loop of the protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE127 Rfz1HA-CS2+ was a gift from Randall Moon (Addgene plasmid # 16797 ; ; RRID:Addgene_16797)