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(Plasmid #175811)


Item Catalog # Description Quantity Price (USD)
Plasmid 175811 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Species
  • Insert Size (bp)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pCAGGS-4493NES (Addgene #138373). pCSII plasmid was developed by the late Dr. Miyoshi (RIKEN BioResource Center).

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The linker region between mKate2 and mKO2 is extremely repeat rich and difficult to validate using Sanger or short read NGS. Requesting scientists should validate expected length using restriction digests in addition to sequencing methods and functionality prior to experimental use.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSIIpuro-4493NES was a gift from Michiyuki Matsuda (Addgene plasmid # 175811 ; ; RRID:Addgene_175811)
  • For your References section:

    Booster, a Red-Shifted Genetically Encoded Forster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools. Watabe T, Terai K, Sumiyama K, Matsuda M. ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941. 10.1021/acssensors.9b01941 PubMed 32101394