pTorPE-FRISZ
(Plasmid #176888)

• Purpose: Far-red fluorescent zinc indicator FRISZ in pTorPE vector
• Depositing Lab: Huiwang Ai
• Publication: Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10.1126/sciadv.add2058. Epub 2023 Mar 1.

Backbone

• Vector backbone: pTorPE
• Backbone size w/o insert (bp): 921
• Total vector size (bp): 5126
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FRISZ
• Species: Synthetic
• Insert Size (bp): 1317
• Promoter: araBAD promoter
• Tags / Fusion Proteins:
  • Twin-Strep-tag (C terminal on insert)
  • Periplasmic expression TorA tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XholI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CATTGTTAACGCCGCGACG
• 3′ sequencing primer: CTTCTGCGTTCTGATTTAATCTGTA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a R362W mutation in the insert at the start of the strep tag. This mutation is not known to affect plasmid function.

Please visit https://www.biorxiv.org/content/10.1101/2022.06.02.494512v1 for bioRxiv preprint.