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Addgene

Anti-Kir2.1 K+ channel [N112B/14R]
(Antibody #182222)

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 182222-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 182222-rAb.T 20 µg of purified recombinant antibody $85 *

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Features

Production & Usage

  • Production Plasmid
    Anti-Kir2.1 K+ channel [N112B/14R]
  • Antigen Plasmid
    Kir2.1-HA/GW1
  • Purification
    Purification from cell culture supernatant using affinity chromatography followed by buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2750703

Terms and Licenses

Target Antigen

Protein Inward rectifier potassium channel 2
Antigen Description Amino acids 390–411 (cytoplasmic C-terminus) of mouse Kir2.1
Antigen Amino Acid Sequence
DSENGVPESTSTDSPPGIDLHN
Species M. musculus (mouse)
Additional Species Reactivity R. norvegicus (rat)
Gene Kcnj2
Alternative Names
  • Inward rectifier K(+) channel Kir2.1
  • IRK-1
  • Potassium channel
  • inwardly rectifying subfamily J member 2
External References

Applications (2)

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Western Blot

1 image
Western Blot image by UC Davis/NIH NeuroMab Facility
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Hybridoma
Sample Species
Other
Result
Pass
1 image
Western Blot image by UC Davis/NIH NeuroMab Facility
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Hybridoma
Sample Species
R. norvegicus (rat)
Result
Fail
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-Kir2.1 K+ channel [N112B/14R] - from James Trimmer (Addgene antibody # 182222 ; http://n2t.net/addgene:182222 ; RRID:AB_2750703)
  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360