Anti-6xHis [N144/14R]
(Antibody
#184180)
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PurposeAnti-6xHis recombinant mouse monoclonal antibody
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Depositing Lab
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Recommended Applications
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Recombinant Antibody | 184180-rAb | 100 µg of purified recombinant antibody | $250 * | ||
Recombinant Antibody trial size | 184180-rAb.T | 20 µg of purified recombinant antibody | $85 * |
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Features
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Source SpeciesM. musculus (mouse)
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IsotypeIgG2a
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Light Chain Typekappa
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Amino Acid Sequences
Sequences (2) — Accept Affinity Reagent Sequence Policy
Production & Usage
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Production PlasmidAnti-6xHis [N144/14R]
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Antigen PlasmidAAV-EF1a-jGCaMP8f-WPRE
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PurificationPurified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
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Storage BufferPBS + 1 mM sodium azide
Delivery
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Concentration0.9–1.1 mg/mL
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StorageUpon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
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ShipmentBlue ice or room temp
Resource Information
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Persistent Resource IdentifierRRID:AB_2750716
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Zeocin® is an InvivoGen trademark.
Trademarks:
Target Antigen
Protein | HIS |
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Antigen Description | Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 NeuroMabs (immunogen was 6xHis fusion protein amino acids 720–804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis (also known as Hexahistidine and Polyhistidine) by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins. |
Species | Synthetic |
Alternative Names |
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Applications (1)
Western Blot
These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Anti-6xHis [N144/14R] - from James Trimmer (Addgene antibody # 184180 ; http://n2t.net/addgene:184180 ; RRID:AB_2750716) -
For your References section:
A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360