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Addgene

Anti-Cav1.2 Ca2+ channel [N263/31R]
(Antibody #184183)

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 184183-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 184183-rAb.T 20 µg of purified recombinant antibody $85 *

* Login to view industry pricing.

Features

Production & Usage

  • Production Plasmid
    Anti-Cav1.2 Ca2+ channel [N263/31R]
  • Antigen Plasmid
    CaV1.2
  • Purification
    Purified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9-1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2909567

Terms and Licenses

Target Antigen

Protein Voltage-dependent L-type calcium channel subunit alpha-1C
Antigen Description Amino acids 808-874 (cytoplasmic loop between repeat II and III) of rat Cav1.2
Antigen Amino Acid Sequence
LARTASPEKKQEVMEKPAVEESKEEKIELKSITADGESPPTTKINMDDLQPSENEDKSPHSNPDTAG
Species R. norvegicus (rat)
Additional Species Reactivity H. sapiens (human),  M. musculus (mouse)
Gene Cacna1c
Alternative Names
  • Calcium channel
  • L type
  • alpha-1 polypeptide
  • isoform 1
  • cardiac muscle
  • Rat brain class C
  • RBC
  • Voltage-gated calcium channel subunit alpha Cav1.2
External References

Applications (2)

Clear filters

Immunohistochemistry

1 image
Immunohistochemistry image by Zhuhao Wu, Weill Cornell Medicine
Submitted By: Zhuhao Wu, Weill Cornell Medicine
Addgene Partner
Antibody Type
Recombinant
Sample Species
M. musculus (mouse)
Result
Pass
1 image
Immunohistochemistry image by James Trimmer, UC Davis
Submitted By: James Trimmer, UC Davis
Addgene Partner
Antibody Type
Recombinant
Sample Species
R. norvegicus (rat)
Result
Pass
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-Cav1.2 Ca2+ channel [N263/31R] - from James Trimmer (Addgene antibody # 184183 ; http://n2t.net/addgene:184183 ; RRID:AB_2909567)
  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360