Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

AAV-FLEX-rev-ChR2-tdtomato Notes

Addgene diagnostic digest 3

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: PciI+AgeI and PciI+NdeI

We expected the following band sizes for this digest: 7.4kb for PciI+AgeI double digest and 4.8kb + 2.6kb for PciI+NdeI double digest. We see the expected pattern.

Digest of 18917 with PvuII, Sma1, and SnaB1

This gel depicts the NEB 1kb ladder and a digestion of the plasmid
AAV-FLEX-rev-ChR2-tdtomato with the following enzymes: SmaI,
PvuII-HF and SnaBI single digests

We expected and saw the following band sizes for this digest:
(1) SmaI - 4.4kb, 2.6kb and 0.4kb
(2) PvuII - 4.9kb and 2.5kb
(3) SnaBI - 4.3kb, 2.8kb and 0.2kb

Addgene Diagnostic Digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 18917.

Expected results:
XmaI: 4379 bp, 2575 bp, 396 bp, 11 bp
PvuII-HF: 4859 bp, 2513 bp
FspI: 3537 bp, 1937 bp, 1172 bp, 726 bp

These results show the expected band sizes.