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pABCb10-GFP
(Plasmid #19031)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19031 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 19031-D50 50 μg of DNA in Tris buffer $495
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DNA 19031-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pIRES
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6092
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATP-binding cassette, sub-family B (MDR/TAP), member 10
  • Alt name
    ABCme
  • Alt name
    ABCb10
  • Alt name
    Abc-mitochondrial erythroid
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2147
  • GenBank ID
    NM_019552
  • Entrez Gene
    Abcb10 (a.k.a. AU016331, Abc-me, Abcb12, C76534)
  • Tag / Fusion Protein
    • HIS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pABCb10-GFP was a gift from Orian Shirihai (Addgene plasmid # 19031 ; http://n2t.net/addgene:19031 ; RRID:Addgene_19031)

  • For your References section:

    Targeting, import, and dimerization of a mammalian mitochondrial ATP binding cassette (ABC) transporter, ABCB10 (ABC-me). Graf SA, Haigh SE, Corson ED, Shirihai OS. J Biol Chem. 2004 Oct 8. 279(41):42954-63. 10.1074/jbc.M405040200 PubMed 15215243
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