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Addgene

Anti-Kir2.3 K+ channel [N25/35R]
(Antibody #191813)

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 191813-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 191813-rAb.T 20 µg of purified recombinant antibody $85 *

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Features

Production & Usage

  • Production Plasmid
    Anti-Kir2.3 K+ channel [N25/35R]
  • Purification
    Purification from cell culture supernatant using affinity chromatography followed by buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2895706

Terms and Licenses

Target Antigen

Protein Inward rectifier potassium channel 4
Antigen Description Amino acids 390–445 (cytoplasmic C-terminus) of human Kir2.3
Antigen Amino Acid Sequence
EAAAAAAVAAGLGLEAGSKEEAGIIRMLEFGSHLDLERMQASLPLDNISYRRESAI
Species H. sapiens (human)
Additional Species Reactivity R. norvegicus (rat)
Gene KCNJ4
Alternative Names
  • HIRK2
  • HRK1
  • Hippocampal inward rectifier (HIR)
  • Inward rectifier K(+) channel Kir2.3 (IRK-3)
  • Potassium channel
  • inwardly rectifying subfamily J member 4
External References

Applications (2)

Clear filters

Immunohistochemistry

1 image
Immunohistochemistry image by UC Davis/NIH NeuroMab Facility
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Hybridoma
Sample Species
R. norvegicus (rat)
Result
Pass

Western Blot

2 images
Western Blot image by UC Davis/NIH NeuroMab Facility
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Hybridoma
Sample Species
R. norvegicus (rat)
Result
Pass

Depositor Comments

Does not cross-react with Kir2.1 or Kir2.2
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-Kir2.3 K+ channel [N25/35R] - from James Trimmer (Addgene antibody # 191813 ; http://n2t.net/addgene:191813 ; RRID:AB_2895706)
  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360