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Anti-Botch [N116/14R]
(Antibody #191818)


Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 191818-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 191818-rAb.T 20 µg of purified recombinant antibody $85 *

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Production & Usage

  • Production Plasmid
    Anti-Botch [N116/14R]
  • Purification
    Purification from cell culture supernatant using affinity chromatography followed by buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide


  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier

Terms and Licenses

Target Antigen

Protein Glutathione-specific gamma-glutamylcyclotransferase 1
Antigen Description Amino acids 1–222 (full-length) of rat Botch1
Antigen Amino Acid Sequence
Species R. norvegicus (rat)
Additional Species Reactivity H. sapiens (human),  M. musculus (mouse)
Gene Chac1
Alternative Names
  • Gamma-GCG 1
  • Blocks Notch protein (Botch)
  • Cation transport regulator-like protein 1
  • Neuroprotective protein 7
External References

Applications (2)

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1 image
Immunocytochemistry image by James Trimmer, UC Davis
Submitted By: James Trimmer, UC Davis
Addgene Partner
Antibody Type
Sample Species

Western Blot

1 image
Western Blot image by UC Davis/NIH NeuroMab Facility
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Sample Species
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-Botch [N116/14R] - from James Trimmer (Addgene antibody # 191818 ; ; RRID:AB_2750705)
  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360