pDA303
(Plasmid
#195199)
-
PurposeOverexpressing 6His-TetR in E. coli.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 195199 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepQE80
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTetR
-
SpeciesE. coli
- Promoter T5 promoter
-
Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer aataggcgtatcacgaggc
- 3′ sequencing primer gcaaccgagcgttctgaac (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDA303 was a gift from Rahul Sarpeshkar (Addgene plasmid # 195199 ; http://n2t.net/addgene:195199 ; RRID:Addgene_195199) -
For your References section:
Rapid modeling of experimental molecular kinetics with simple electronic circuits instead of with complex differential equations. Deng Y, Beahm DR, Ran X, Riley TG, Sarpeshkar R. Front Bioeng Biotechnol. 2022 Sep 28;10:947508. doi: 10.3389/fbioe.2022.947508. eCollection 2022. 10.3389/fbioe.2022.947508 PubMed 36246369
Map uploaded by the depositor.
