Anti-6xHis [N144/14] - Chimeric
PurposeAnti-6xHis chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a rabbit IgG Fc.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Recombinant Antibody||196562-rAb||100 µg of purified recombinant antibody||$250 *|
|Recombinant Antibody trial size||196562-rAb.T||20 µg of purified recombinant antibody||$85 *|
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Production & Usage
PurificationPurification from cell culture supernatant using affinity chromatography followed by buffer exchange with centrifugal columns.
Storage BufferPBS + 1 mM sodium azide
Terms and Licenses
|Antigen Description||Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 NeuroMabs (immunogen was 6xHis fusion protein amino acids 720–804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis (also known as Hexahistidine and Polyhistidine) by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.|
These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Anti-6xHis [N144/14] - Chimeric was a gift from Melina Fan, James Trimmer (Addgene antibody # 196562 ; http://n2t.net/addgene:196562 ; RRID:AB_2928072)
For your References section:A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360