kiCAP-AAV-M.Mus.2
(Plasmid
#196682)
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PurposeRep/Cap plasmid for the production of M.Mus.2, an AAV capsid with CNS tropism in mice.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196682 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57-Kan
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 9398
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Modifications to backboneBackbone modifications as in kiCAP-AAV-BI30
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
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Alt nameM.Mus.2
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SpeciesSynthetic
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Insert Size (bp)2232
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MutationREQQKLW insert between amino acids 588 and 589 of AAV9 VP1, A587D, Q588G
- Promoter p41
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsiWI (not destroyed)
- 3′ cloning site AgeI (destroyed during cloning)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
kiCAP-AAV-M.Mus.2 was a gift from Pardis Sabeti (Addgene plasmid # 196682 ; http://n2t.net/addgene:196682 ; RRID:Addgene_196682) -
For your References section:
Systemic administration of novel engineered AAV capsids facilitates enhanced transgene expression in the macaque CNS. Stanton AC, Lagerborg KA, Tellez L, Krunnfusz A, King EM, Ye S, Solomon IH, Tabebordbar M, Sabeti PC. Med (N Y). 2022 Nov 15:S2666-6340(22)00456-1. doi: 10.1016/j.medj.2022.11.002. 10.1016/j.medj.2022.11.002 PubMed 36417917