Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20334||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeAP reporter
Growth in Bacteria
Gene/Insert namebeta catenin reporter
SpeciesH. sapiens (human)
Insert Size (bp)2200
/ Fusion Protein
- AP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site HpaI (unknown if destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe idea for the construct is from Hans Clevers lab, who designed the original TOPflash.
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SuperTopAP was a gift from Connie Cepko (Addgene plasmid # 20334 ; http://n2t.net/addgene:20334 ; RRID:Addgene_20334)
For your References section:Wnt2b/beta-catenin-mediated canonical Wnt signaling determines the peripheral fates of the chick eye. Cho SH, Cepko CL. Development. 2006 Aug . 133(16):3167-77. 10.1242/dev.02474 PubMed 16854977