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SuperTopAP
(Plasmid #20334)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20334 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    beta catenin reporter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Tag / Fusion Protein
    • AP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site HpaI (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The idea for the construct is from Hans Clevers lab, who designed the original TOPflash.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SuperTopAP was a gift from Connie Cepko (Addgene plasmid # 20334 ; http://n2t.net/addgene:20334 ; RRID:Addgene_20334)
  • For your References section:

    Wnt2b/beta-catenin-mediated canonical Wnt signaling determines the peripheral fates of the chick eye. Cho SH, Cepko CL. Development. 2006 Aug . 133(16):3167-77. 10.1242/dev.02474 PubMed 16854977