ptfLC3
(Plasmid #21074)

• Purpose: Mammalian expression of rat LC3 fused to mRFP and EGFP
• Depositing Lab: Tamotsu Yoshimori
• Depositing Organization: The University of Osaka
  Located in Japan
• Publication: Kimura et al Autophagy. 2007;3(5):452-60.
• Citations: 330 Articles

Backbone

• Vector backbone: pEGFP-C1+mRFP
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: microtubule-associated protein 1 light chain 3 beta
• Alt name: Map1lc3b
• Alt name: LC3
• Alt name: LC3b
• Species: R. norvegicus (rat)
• Insert Size (bp): 738
• GenBank ID: U05784
• Entrez Gene: Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
• Tags / Fusion Proteins:
  • mRFP (N terminal on backbone)
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharian DA, Tsien RY, A monomeric red fluorescent protein, Proc Natl Acad Sci USA. 2002, 99(12):7877-82

NOTE FROM AUTHORS: We found several alterations between our plasmid and the version on NCBI [Map1lc3b; https://www.ncbi.nlm.nih.gov/gene/64862) ].
Our original clone may be a kind of variant.
In the C-terminal end of tfLC3, the stop codon is removed, which causes an additional 17 amino acid residues.
However, the C-terminal end from Gly120, including the 17 amino acids, is immediately cut off by Atg4 protease after the translation.
Therefore that sequence variant will not affect the function of the protein.

DNA (Catalog # 21074-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 21074-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.