5'-3xFLAG Tcf12-exon minimal CMV pCDNA5
(Plasmid
#212076)
-
PurposeLR vector for integration of Tcf12-exon into N2a FRT rtTA3 expression cells
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 212076 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbone5'-3xFLAG minimal CMV pCDNA5
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTcf12-exon
-
Alt nameNP_001240791.1
-
SpeciesM. musculus (mouse)
-
Entrez GeneTcf12 (a.k.a. A130037E08Rik, ALF1, HEB, HEBAlt, HTF-4, HTF4, ME1, REB, bHLHb20)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
5'-3xFLAG Tcf12-exon minimal CMV pCDNA5 was a gift from Benjamin Blencowe (Addgene plasmid # 212076 ; http://n2t.net/addgene:212076 ; RRID:Addgene_212076) -
For your References section:
Systematic analysis of alternative exon-dependent interactome remodeling reveals multitasking functions of gene regulatory factors. Roth JF, Braunschweig U, Wu M, Li JD, Lin ZY, Larsen B, Weatheritt RJ, Gingras AC, Blencowe BJ. Mol Cell. 2023 Dec 7;83(23):4222-4238.e10. doi: 10.1016/j.molcel.2023.10.034. 10.1016/j.molcel.2023.10.034 PubMed 38065061
