|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21595||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneR6K origin PCR fragment
- Backbone size w/o insert (bp) 300
Vector typeWorm Expression ; Adds unc-119 marker to Amp resistant plasmids
Growth in Bacteria
Growth instructionsRequires pir expressing bacterial strain such as EC100 pir-116
Copy numberLow Copy
Alt namekanamycin resistance
SpeciesC. elegans (nematode)
Insert Size (bp)3200
Entrez Geneunc-119 (a.k.a. CELE_M142.1)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHi (not destroyed)
- 5′ sequencing primer GCACTTTTCGGGGAAATGTGC
- 3′ sequencing primer GGTCTGACAGTTACCAATGCTTAATC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid has small deletions within the KanR gene. We generated these fragments by PCR so it is definitely possible that these were generated during amplification, these changes must not impair function as the construct is Kan resistant both as a plasmid and following recombination.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:punc-119c was a gift from Al Fisher (Addgene plasmid # 21595 ; http://n2t.net/addgene:21595 ; RRID:Addgene_21595)
For your References section:Retrofitting Ampicillin Resistant Vectors by Recombination for use in Generating C. elegans Transgenic Animals by Bombardment. Ferguson AA, Fisher AL. Plasmid. 2009 Jun 8. ():. 10.1016/j.plasmid.2009.06.001 PubMed 19520111