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Addgene

PPE18-ALFA
(Plasmid #231247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBB115
  • Backbone manufacturer
    Bryson Lab
  • Backbone size w/o insert (bp) 6512
  • Total vector size (bp) 7754
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPE18-ALFA
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
    1254
  • GenBank ID
    886073
  • Entrez Gene
    PPE18 (a.k.a. Rv1196, mtb39a)
  • Promoter hsp60
  • Tag / Fusion Protein
    • ALFA Tag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PPE18-ALFA was a gift from Bryan Bryson (Addgene plasmid # 231247 ; http://n2t.net/addgene:231247 ; RRID:Addgene_231247)
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