pSKI
(Plasmid
#232484)
-
PurposepSKI can be used for single-copy transgene expression within the SKI PLACE System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232484 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepPD95_75
- Backbone size w/o insert (bp) 2598
- Total vector size (bp) 4728
-
Vector typeWorm Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namepSKI
-
SpeciesC. elegans (nematode)
-
Insert Size (bp)2130
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.12.06.627295 for bioRxiv preprint.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSKI was a gift from Carlos Silva García (Addgene plasmid # 232484 ; http://n2t.net/addgene:232484 ; RRID:Addgene_232484) -
For your References section:
A Single-Copy Knock-In System: One Plasmid to Target All Chromosomes in C. elegans. Dinneen E, Dasgupta P, Sharma A, Nisaa K, Silva-Garcia CG. G3 (Bethesda). 2025 Sep 19:jkaf220. doi: 10.1093/g3journal/jkaf220. 10.1093/g3journal/jkaf220 PubMed 40973646
Map uploaded by the depositor.
