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Addgene

pAAV-hSyn-GRAB-HaloDA1.0
(Plasmid #234408)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234408 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0
  • Alt name
    GRAB_HaloDA1.0
  • Alt name
    HaloDA1.0
  • Species
    H. sapiens (human)
  • Promoter hSyn

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACGGGCGCGACCATCTGCGC
  • 3′ sequencing primer CATTAAAGCAGCGTATCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.12.22.629999 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-GRAB-HaloDA1.0 was a gift from Yulong Li (Addgene plasmid # 234408 ; http://n2t.net/addgene:234408 ; RRID:Addgene_234408)

  • For your References section:

    In vivo multiplex imaging of dynamic neurochemical networks with designed far-red dopamine sensors. Zheng Y, Cai R, Wang K, Zhang J, Zhuo Y, Dong H, Zhang Y, Wang Y, Deng F, Ji E, Cui Y, Fang S, Zhang X, Zhang K, Wang J, Li G, Miao X, Wang Z, Yang Y, Li S, Grimm J, Johnsson K, Schreiter E, Lavis L, Chen Z, Mu Y, Li Y. Science. 2025 Jun 5;388: 6751. doi: https://doi.org/10.1126/science.adt7705. 10.1126/science.adt7705 PubMed 39763912
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