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Addgene

HP17deltaP8
(Plasmid #235447)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235447 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC101
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Coding part of the M13KO7 helper phage genome, except gene VIII
  • Species
    Escherichia coli

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

JM101 (working strain for phagemid transfer). Please visit https://doi.org/10.1101/2024.08.28.610043 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HP17deltaP8 was a gift from Yolanda Schaerli (Addgene plasmid # 235447 ; http://n2t.net/addgene:235447 ; RRID:Addgene_235447)

  • For your References section:

    Engineering intercellular communication using M13 phagemid and CRISPR-based gene regulation for multicellular computing in Escherichia coli. Kusumawardhani, H., Zoppi, F., Avendaño, R. et al.. Nat Commun 16, 3569 (2025) 10.1038/s41467-025-58760-z
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