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Addgene

pcDNA3 2xFLAG-2xSTREP E2F7
(Plasmid #236442)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236442 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2F7
  • Species
    H. sapiens (human)
  • Entrez Gene
    E2F7
  • Tag / Fusion Protein
    • FLAG-FLAG-STREP-STREP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 2xFLAG-2xSTREP E2F7 was a gift from Michele Pagano (Addgene plasmid # 236442 ; http://n2t.net/addgene:236442 ; RRID:Addgene_236442)

  • For your References section:

    Cyclin F Controls Cell-Cycle Transcriptional Outputs by Directing the Degradation of the Three Activator E2Fs. Clijsters L, Hoencamp C, Calis JJA, Marzio A, Handgraaf SM, Cuitino MC, Rosenberg BR, Leone G, Pagano M. Mol Cell. 2019 Jun 20;74(6):1264-1277.e7. doi: 10.1016/j.molcel.2019.04.010. Epub 2019 May 23. 10.1016/j.molcel.2019.04.010 PubMed 31130363
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