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Addgene

NanoBRET Assay Vector, CRAF(FL)-NanoLuc
(Plasmid #236870)

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Item Catalog # Description Quantity Price (USD)
Plasmid 236870 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pFC223C NanoLuc(R) CMV-Neo BiBRET-Balanced Vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRAF
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
  • Promoter CMV
  • Tag / Fusion Protein
    • NanoLuc (R) (C terminal on backbone)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NanoBRET Assay Vector, CRAF(FL)-NanoLuc was a gift from Promega Early Access Program (Addgene plasmid # 236870)
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