NanoBRET Assay Vector, MYC
(Plasmid
#237142)
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PurposeExpress Myc-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 237142 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
DNA | 237142-D20 | 20 μg of DNA in Tris buffer | $393 * |
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Backbone
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Vector backbonepFC32K Nluc CMV-neo Flexi(R) Vector
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMyc
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SpeciesH. sapiens (human)
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MutationNone
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
- Promoter CMV
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Tag
/ Fusion Protein
- NanoLuc (R) (C terminal on backbone)
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Information for DNA (Catalog # 237142-D20) ( Back to top)
Purpose
DNA aliquots are suitable for use in molecular biology and scientific research
Delivery
- Amount 20 μg
- Guaranteed Concentration 500 ng/μl +/- 25 ng/μl
- Pricing $393 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
Quality Control
Addgene has verified this plasmid using Next Generation Sequencing. Review the sequencing results. Addgene does not test for endotoxin levels on these samples.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NanoBRET Assay Vector, MYC was a gift from Promega Early Access Program (Addgene plasmid # 237142 ; http://n2t.net/addgene:237142 ; RRID:Addgene_237142)