pMB-Tl-ADPHK
(Plasmid #237469)

• Purpose: Expression of His-tagged Thermococcus litoralis ADP hexokinase
• Depositing Lab: Michael Baker
• Publication: ADP-Hexokinase expression plasmid (unpublished)

Backbone

• Vector backbone: pET51(b)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5218
• Total vector size (bp): 6586
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ADP hexokinase
• Alt name: ADP-HK
• Species: Thermococcus litoralis DSM 5473
• Insert Size (bp): 1512
• GenBank ID: WP_171814849.1
• Promoter: T7
• Tags / Fusion Proteins:
  • C-terminal 10 His-tag (C terminal on backbone)
  • N-terminal strep tag (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • pET51b_ADPHexTl.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are discrepancies between the Addgene NGS sequence and the depositor reference: 2 1-bp SNPs in ADPHex T. lit that cause AA changes P21V and F492S. The depositor has confirmed these do not impact plasmid function.