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Dual-sgRNA CRISPRi Library for PARP Inhibitor Response
(Pooled Library #238134)

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  • Purpose

    This library contains dual-sgRNAs targeting gene pairs encoding factors involved in DNA repair and PARP inhibitor response (two sgRNAs per gene; eight dual-sgRNA constructs per gene pair). The library can be used in experiments for mapping genetic interaction networks in DNA repair and PARP inhibitor response.

    To perform genetic interaction mapping, transduce the dual-sgRNA CRISPRi library into cells expressing dCas9-KRAB to knockdown the target gene pairs.

  • Vector Backbone

    dual-sgRNA CRISPRi PARP_backbone (GB, 19 KB) - does not express Cas9

    pJL051 (Plasmid #242609) - does not express Cas9

    pJL052 (Plasmid #242610) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 238134 Dual-sgRNA CRISPRi Library for PARP Inhibitor Response† 1 $1430 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pHR-SFFV-dCas9-BFP-KRAB (Addgene #46911) or any other plasmid(s) or cell lines expressing dCas9-KRAB expressing plasmids.

Library Details

  • Species
    Human
  • Gene pairs
    147,153
  • gRNAs
    1,301,881 dual-sgRNA constructs in theory; 1,301,379 detected by deep sequencing
  • Controls
    3,025 non-targeting dual-sgRNA constructs (combinations from 55 single non-targeting sgRNAs)
  • Lentiviral generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

A circle labeled “GI library gene targets 543” with smaller circles overlapping its edge labeled “Cell Cycle 91”, “DNA replication 56”, “Immune response 54”, “Cellular senescence 45”, “Transport 38”, “mRNA processing 34”, “Signaling 29”, and “Biorhythms 24”. An additional larger overlapping circle labeled “DNA damage repair 145” has additional spokes radiating out from it, and these are labeled “Homologous recombination 65”, “Telomere maintenance 37”, “Non-homologous end joining 32”, “Base excision repair 26”, “Interstrand cross-link repair 22”, “Nucleotide excision repair 19”, and “Mismatch repair 17”.

Figure 1: Functional annotation of genes included in the dual-sgRNA CRISPRi Library for PARP Inhibitor Response. Genes may appear in multiple categories.

Please visit https://doi.org/10.1101/2023.08.19.553986 (Link opens in a new window)for bioRxiv preprint.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dual-sgRNA CRISPRi Library for PARP Inhibitor Response was a gift from Brittany Adamson (Addgene #238134 ; http://n2t.net/addgene:238134 ; RRID:Addgene_238134)

  • For your References section:

    Mapping the Genetic Interaction Network of PARP inhibitor Response. Simpson D, Ling J, Jing Y, Adamson B. bioRxiv [Preprint]. 2023 Aug 20:2023.08.19.553986. doi: 10.1101/2023.08.19.553986. bioRxiv 2023.08.19.553986
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