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Addgene

pHS2002
(Plasmid #239832)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239832 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NovaIscB omegaRNA scaffold
  • Species
    Human gut metagenome assembly
  • Mutation
    Replacement of A12-A57 with GAAA in omegaRNA scaffold and 17 nt truncation at the 3' end of omegaRNA scaffold
  • Promoter human U6

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS2002 was a gift from Feng Zhang (Addgene plasmid # 239832 ; http://n2t.net/addgene:239832 ; RRID:Addgene_239832)

  • For your References section:

    Evolution-guided protein design of IscB for persistent epigenome editing in vivo. Kannan S, Altae-Tran H, Zhu S, Xu P, Strebinger D, Oshiro R, Faure G, Moeller L, Pham J, Mears KS, Ni HM, Macrae RK, Zhang F. Nat Biotechnol. 2025 May 7. doi: 10.1038/s41587-025-02655-3. 10.1038/s41587-025-02655-3 PubMed 40335752
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