peGFP-HDAC1 (pc2447)
(Plasmid #240148)

• Purpose: Expression vector containing a fusion protein of eGFP and human HDAC1.
• Depositing Lab: Cristina Cardoso
• Publication: Rausch et al Nucleic Acids Res. 2020 Dec 16;48(22):12751-12777. doi: 10.1093/nar/gkaa1124.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6204
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HDAC1
• Species: H. sapiens (human)
• Insert Size (bp): 1442
• Entrez Gene: HDAC1 (a.k.a. GON-10, HD1, KDAC1, RPD3, RPD3L1)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Other
• 5′ sequencing primer: AGAAGCGCGATCACATG

Resource Information

• Supplemental Documents:
  • peGFP-HDAC1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS finds L41M in HDAC1. The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.