pCMV-LAMP1-iLID-Halo
(Plasmid
#247296)
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PurposeMammalian expression of LAMP1-iLID-Halo fusion for light-induced recruitment to late endosomes/lysosomes.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCMV
- Total vector size (bp) 1419
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLAMP1-iLID-Halo
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SpeciesH. sapiens (human)
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Insert Size (bp)1419
- Promoter CMV
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Tag
/ Fusion Protein
- HaloTag
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-LAMP1-iLID-Halo was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 247296 ; http://n2t.net/addgene:247296 ; RRID:Addgene_247296) -
For your References section:
Secretome translation shaped by lysosomes and lunapark-marked ER junctions. Choi H, Liao YC, Yoon YJ, Grimm J, Wang N, Lavis LD, Singer RH, Lippincott-Schwartz J. Nature. 2025 Nov 5. doi: 10.1038/s41586-025-09718-0. 10.1038/s41586-025-09718-0 PubMed 41193816
