Skip to main content
Addgene
Open Menu

SpeckSeq Library
(Pooled Library #248187)

Print
  • Purpose

    The SpeckSeq library is a pooled lentiviral plasmid library containing 228 distinct variants of the human MEFV gene (plus 11 wild-type MEFV controls), which encodes the inflammasome sensor pyrin. Each MEFV variant is linked to a unique barcode. It enables scientists to perform screens to study the functional effects of 228 distinct MEFV variants.

  • Vector Backbone

    pInducer20 (Plasmid #44012)

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 248187 SpeckSeq MEFV variant pooled library 1 $418 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • MEFV variants
    228 variants, 11 wild-type controls
  • Lentiviral generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    15 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

A graphical representation of MEFV variant distribution. Variant number is on the X axis, from 0 to over 200 (to about 250). Variant frequent (percent of total) is on the Y axis, labeled 0.0 to 2.0. Variants are represented as black dots. Most dots lie in a gray box between 0.1 and 0.92 variant frequency. The red horizontal line indicates the expected frequency per variant, approximately 0.4%.
Figure 1: Distribution of each variant in the pInducer20 plasmid as determined by sequencing of MEFV variant barcodes by Next Generation Sequencing. Each dot represents the frequency of an individual variant in the population from one experiment. The red line represents the expected value assuming equal distribution of variants (y = 0.4%). The grey zone is delineated by values Q3 + 1.5 IQR (upper limit), and Q1 - 1.5 x IQR (lower limit).
The MEFV barcoded library (n = 239) represented visually using colored lines for the MEFV CDS, a flag icon for the DNA bar code, and an X for the mutation. Examples of variants are shown from top to bottom, with MEFV WT 1 to 11 (no mutation), followed by MEFV variant 1 (mutation near start of CDS) and MEFV variant 2 (mutation slightly downstream of variant 1). Other examples include a variant carrying M694V, another carrying S242R, as well as MEFV variants 227 (mutation near the middle of the coding region) and 228 (mutation slightly upstream of variant 227).
Figure 2: Visual representation of the MEFV variants.
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpeckSeq library was a gift from Thomas Henry (Addgene #248187 ; http://n2t.net/addgene:248187 ; RRID:Addgene_248187)

  • For your References section:

    SpeckSeq enables high-throughput functional stratification of MEFV variants in autoinflammatory diseases. Bronnec P, Dalmon S, Briand C, Allatif O, Broly M, Marcotte M, Lombardi G, Barthes K, Martel N, Hughes S, Gillet B, Milhavet F, Atilgan A, Bachelez H, Palmeri S, Prigione I, Madrange M, Savey L, Moutschen M, Jeru I, El Moussaoui M, Belot A, Sbidian E, Carbone A, Jamilloux Y, Gattorno M, Smahi A, Georgin-Lavialle S, Boursier G, Magnotti F, Henry T. J Exp Med. 2026 Feb 2;223(2):e20251065. doi: 10.1084/jem.20251065. PubMed 41335224
Related items
Additional resources